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Serology biomarker miR-22 for testing ovarian tumor and application thereof

A technology for biomarkers and ovarian tumors, applied in the determination/testing of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc.

Inactive Publication Date: 2013-01-09
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no reports on the application of serum / plasma microRNA in auxiliary judgment and risk assessment of ovarian diseases. If specific or abnormally expressed serum / plasma microRNA related to Markers, and the development of corresponding detection kits, will greatly improve the detection level of ovarian tumor diseases

Method used

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  • Serology biomarker miR-22 for testing ovarian tumor and application thereof
  • Serology biomarker miR-22 for testing ovarian tumor and application thereof
  • Serology biomarker miR-22 for testing ovarian tumor and application thereof

Examples

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Effect test

example 1

[0166] Example 1. Select 100 patients who have been diagnosed with malignant ovarian tumors (including 41 cases of serous adenocarcinoma, 32 cases of mucinous adenocarcinoma, 15 cases of endometrioid adenocarcinoma, and 12 cases of clear cell carcinoma), breast cancer patients, lung cancer patients 30 patients with stomach cancer, liver cancer, colon cancer, and prostate cancer, and 30 normal people. Peripheral blood was collected on an empty stomach in the early morning, and the entire detection method was the same as above. The results are shown in Table 8 below:

[0167] Table 8 Proportion of RQ ≥ 2 / < 2 in different cancer cases

[0168]

[0169] From the data in the above table, it can be seen that the proportion of RQ ≥ 2 in ovarian cancer is significantly higher than that of other cancers, that is, miR-22 is specifically expressed in ovarian cancer and can be used as an auxiliary individual ovarian tumor screening , Early detection of ovarian tumors and the basis for...

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PUM

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Abstract

The invention discloses a serology biomarker miR-22 for testing ovarian tumor. The miRNA sequence of miR-22: aagcugccaguugaagaacugu. The invention further discloses an application of the miR-22: a kit for screening ovarian tumor or assisting ovarian tumor pathology identification and clinical diagnosis is manufactured. The kit comprises a reverse transcription system and a primer system or a primer probe system, wherein the reverse transcription system comprises reverse transcriptase, a reverse transcription system buffer and ribonucleic acid (RNA) enzyme inhibitors; and the primer system is composed of miR-22 stem-loop reverse transcription primers, cDNA amplification primers and miR-16 stem-loop reverse transcription primers and amplification primers; and the primer probe system is composed of miR-22 stem-loop reverse transcription primers, cDNA amplification primers, a probe and miR-16 stem-loop reverse transcription primers, amplification primers and a probe.

Description

technical field [0001] The invention belongs to the fields of biotechnology and medicine, and in particular relates to a biomarker for ovarian cancer tumors—serum microRNA-22 (miR-22), its screening method and its ability to assist individual ovarian tumor screening and clinical diagnosis and Application in disease progression. Background technique [0002] Ovarian cancer (ovarian carcinoma) is one of the common tumors of female reproductive organs, most of which are caused by gene mutations in ovarian epithelial cells and inclusion cyst tissue epithelial cells under the action of various carcinogenic factors, resulting in uncontrolled cell proliferation. Ovarian cancer is a gynecological malignancy with a very high mortality rate. Its histological forms are diverse, and it is a highly heterogeneous tumor with high incidence, slow disease progression, and difficult to detect early symptoms; Strong, long natural lifespan and so on. In clinical examination, CA-125 level dete...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/113
Inventor 丁先锋纪婷唐文杲蒋理想李彩霞
Owner ZHEJIANG SCI-TECH UNIV
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