Application of Gypensapogenin A to preparing drugs for treating yellow fever virus infection
A yellow fever virus and drug technology, applied in the field of biomedicine, to achieve huge social benefits and research value
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Embodiment 1
[0036] Embodiment 1: the preparation of the compound Gypensapogenin A tablet involved in the present invention:
[0037] Take 20 grams of compound Gypensapogenin A, add 180 grams of conventional excipients for tablet preparation, mix well, and make 1000 tablets with a conventional tabletting machine.
Embodiment 2
[0038] Embodiment 2: The preparation of the compound Gypensapogenin A capsules involved in the present invention:
[0039] Get 20 grams of compound Gypensapogenin A, add conventional auxiliary materials such as starch 180 grams for the preparation of capsules, mix well, and pack into capsules to make 1000 tablets.
[0040] The following pharmacodynamic experiments will further illustrate its drug activity.
experiment example 1
[0042] A kind of application of Gypensapogenin A in the preparation treatment or prevention yellow fever virus infection medicine, its steps are:
[0043] A. Toxicity test of Gypensapogenin A on Vero cells
[0044] Vero cells (African green monkey kidney cells) are susceptible cells to YFV.
[0045] The experimental steps are as follows:
[0046] 1: Inoculate Vero cells: Use DMEM medium containing 10% (v / v) fetal bovine serum to make a single cell suspension, inoculate 1000-10000 cells per well into a 96-well cell culture plate, and inoculate a volume of 100ul per well ;
[0047] 2: Culture Vero cells: at 37°C, 5% (v / v) CO 2 Under culture conditions, cultivate for 2 days;
[0048] 3: Add Gypensapogenin A: Discard the DMEM medium in each well, add 100ul to each well and dilute to the corresponding concentration (0uM, 0.4uM, 1.2 uM, 3.7uM, 11uM, 33uM, 100uM, 300uM) Gypensapogenin A, add 100ul of DMEM medium without 10% (v / v) fetal bovine serum to the control well;
[0049]...
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