Method for constructing full-biological tissue engineering blood vessel

Abstract

The invention discloses a method for constructing a full-biological tissue engineering blood vessel. The method is characterized in that an acellular tissue matrix is prepared from a sheep carotid artery; endothelial progenitor cells, smooth muscle progenitor cells and adipose-derived stem cells are respectively used as seed cells for constructing tunica intima vasorum, tunica media vasorum and tunica externa; and dynamic processes of wall shear stress, annular tension and axial tensile stress during construction of the blood vessel are realized by a bioreactor. The optimized acellular tissue matrix and optimized seed cell sources are adopted, and standard 3-D (three-dimensional) stress and dynamic regulation are realized in a construction process, so that the structure, mechanical characteristics and biological activities of the constructed tissue engineering blood vessel are close to those of a natural blood vessel. Accordingly, the method has industrial production and clinical application prospects, and a foundation is laid for the tissue engineering blood vessel to access markets.

Description

technical field [0001] The invention belongs to the technical field of biological tissue engineering, and provides a tissue engineering blood vessel construction scheme using biological and autologous cells as raw materials. Arterial bypass, radial artery-venous short circuit, deep vein replacement, etc. require vascular grafting as a substitute for natural blood vessels. Background technique [0002] There are more than 5 million cases of coronary artery bypass grafting and vascular bypass surgery carried out in the world every year, requiring a large amount of vascular graft materials. At present, autologous blood vessel transplantation is mainly used. However, in some patients, the blood vessels available for transplantation have already been used or have undergone pathological changes, so artificial materials can only be used to replace blood vessels. However, artificial blood vessels have disadvantages such as poor elasticity and low patency rate. Solving the source of ...

AI Technical Summary

Problems solved by technology

However, another kind of vascular stress—the axial tensile force on arterial blood vessels produced by the beating of the heart has been ignored, which may be an important reason why tissue engineering blood vessels are difficult to be applied clinically at present.

Moreover, the mechanical environment during vascular construction is a dynamic process for which no standardized studies have been performed

[0009] To sum up, under the existing vascular construction technology, there is no tissue-engineered blood vessel with mass production and clinical application potential. The constructed engineered blood vessels are not comparable to natural blood vessels in terms of structure and strength; second, the collection of seed cells for constructing blood vessels is subject to many technical and ethical restrictions

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