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Fermentation process of cephalosporin C

A technology of cephalosporin and fermentation method, which is applied in the fields of bioengineering and microbiology, and can solve problems such as the decline of soybean oil utilization capacity, the influence of cephalosporin C extraction, and the poor defoaming effect of soybean oil.

Active Publication Date: 2013-01-16
SHANXI WEIQIDA PHARMA IND +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the later stage of fermentation, the liquid surface is prone to foaming. Since the defoaming effect of soybean oil is poor, it is necessary to add more soybean oil to effectively control the liquid level. Too much soybean oil in the fermentation broth will affect the extraction of cephalosporin C

Method used

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  • Fermentation process of cephalosporin C
  • Fermentation process of cephalosporin C
  • Fermentation process of cephalosporin C

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Embodiment 1, the influence of different kinds of defoamers on the fermentation of cephalosporin C

[0065] On the shake flask culture level, select five antifoaming agents (A, D belong to polyether antifoaming agents, B, E belong to silicone antifoaming agents, and C belong to high carbon alcohol antifoaming agents) according to 0.25% ( The ratio of v / v) was added to the fermentation medium (soybean oil was initially added, and the amount added was the same as that of the control group). The effect of anti-foaming agents on the fermentation of cephalosporin C, see the results figure 1 .

[0066] Among the five defoamers, the potency (u / mL) of A, C, and D was higher than that of the control, and that of B, E was lower than that of the control. Among them, D has the highest titer and E has the lowest potency. It can be seen that polyoxyethylene oxypropylene glycerin has little influence on the fermentation of cephalosporin C (even has a synergistic effect), while sili...

Embodiment 2

[0069] Embodiment 2, the impact of adding defoamer on the fermentation of cephalosporin C in the fermentation process

[0070] In a 50L fermenter, fermentation was carried out according to the aforementioned "cultivation in a 50L fermenter". Select D defoamer to carry out the supplementary experiment of defoamer, start to add defoamer intermittently through peristaltic pump at 100 hours, when the foam of fermentation broth rises to the position where the total amount of foam and fermentation broth is 35L (observed through the fermenter sight glass ) began to add anti-foaming agent, adding 1.5g of anti-foaming agent each time (that is, the total amount of 0.043g / L foamy fermentation broth). Ammonium sulfate stopped adding after 50 hours. Add soybean oil at a rate of 30g / hr at 50 hours, stop adding soybean oil at 100 hours, and start adding defoamer. The amount of soybean oil supplementation was reduced by about 25% compared with the control group. Control the fermentation li...

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Abstract

The invention relates to a fermentation process of cephalosporin C. At a later fermentation stage, the cephalosporin C is defoamed by supplementing a defoaming agent instead of soya-bean oil, so that cost of raw materials can be reduced by 15% under a condition that valence increasing and filtering velocity are not influenced.

Description

technical field [0001] The invention belongs to the fields of bioengineering and microbiology; more specifically, the invention relates to a cephalosporin C fermentation method. Background technique [0002] Cephalosporium acremonuim is produced by Cephalosporium acremonuim. It is the product of the continuation and development of the primary metabolism of Cephalosporium acremonuim under specific conditions. It is another important β-lactam antibiotic after penicillin. [0003] At present, soybean oil is used as the main carbon source in the industrial fermentation process of cephalosporin C, and it also plays the role of defoaming. In the later stage of fermentation, the liquid surface is prone to foaming. Since the defoaming effect of soybean oil is poor, it is necessary to add more soybean oil to effectively control the liquid level. Too much soybean oil in the fermentation broth will affect the extraction of cephalosporin C. [0004] Therefore, there is a need in the a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P35/06C12R1/75
Inventor 黎亮张俊波储炬李树友庄英萍苗瑞春张嗣良陆俊
Owner SHANXI WEIQIDA PHARMA IND
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