Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Bacterial strain for generating anisic acid by converting anethole, and screening method and application of bacterial strain

A screening method, the technology of anisic acid, applied in the field of transforming anethole to produce anisic acid strains and fermenting the field of producing anisic acid, can solve the problems of many by-products, pollute the environment, waste resources, etc., and achieve the effect of optimizing fermentation conditions

Inactive Publication Date: 2013-01-23
GUANGXI UNIV
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the production of anisaldehyde and anisic acid is mainly chemical synthesis method (mainly oxidation method), which has low productivity and many by-products, which not only wastes resources but also pollutes the environment.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bacterial strain for generating anisic acid by converting anethole, and screening method and application of bacterial strain
  • Bacterial strain for generating anisic acid by converting anethole, and screening method and application of bacterial strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Screening of strains

[0029] 5 g of each collected soil sample was weighed and added to the sterilized enrichment medium, and cultured on a shaker at 30° C. at 200 rpm for 72 hours. Transfer the bacterial solution to fresh enrichment culture according to 10% inoculum amount, after three times of concentration gradient enrichment culture of anethole, take 1mL enrichment culture, carry out gradient dilution with sterile water, take 10 -5 、10 -6 and 10 -7 Three concentrations, spread on the minimal medium plate, and incubate upside down at 30°C for 3-5 days. Pick a single colony on the plate, inoculate it into a 10ml basic medium finger flask, add 4g / L anethole, culture on a shaker at 200rpm at 30°C for 48h, and streak on the basic medium plate; repeat several times for purification; Inoculate the purified strain into a finger flask containing 10mL of fermentation medium, add 4g / L anethole, transform at 30°C, shaker at 200rpm for 48h, and perform qualitative analysis o...

Embodiment 2

[0043] Determination of the optimal temperature and transformation time of bacterial strains

[0044] The strain with the preservation number CCTCC NO.M 2012060 was streaked on the seed slant medium, cultured in an incubator at 28°C, and then transferred to a liquid seed medium and cultured on a shaker at 28°C to the logarithmic growth phase. Inoculate 10% of the inoculum into the fermentation medium, carry out fermentation and culture in shaking tables at different temperatures, and extract the fermentation liquid after 24 hours and analyze it by gas chromatography.

[0045] Seed medium (g / L): Tryptone 10g, yeast extract 5g, NaCl 10g, distilled water 1000mL, 121°C, sterilized for 20min; pH 7.0, 30°C, shaker speed 200 rpm, culture time about 12h.

[0046] Fermentation medium (g / L): glucose 20g, peptone 10g, beef extract 2.5g, KH 2 PO 4 1.0g, NaCl 0.5g, MgSO 4 0.5g, FeSO 4 0.01g, anethole 4g / L, distilled water 1000mL; 115°C, sterilize for 20min; pH 7.0, fermentation tempera...

Embodiment 3

[0061] Optimization of fermentation conditions

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a bacterial strain for generating anisic acid by converting anethole, and a screening method and application of the bacterial strain. The bacterial strain BurkholderiaspWGB31 which is high in yield of anisic acid can be obtained by the following steps of: enriching by using enrichment mediums with different anethole concentration gradients in soil of anise forest, inoculating to a 5g / l anethole screening culture medium, performing plate screening, transferring to a seed culture medium, performing shake-flask culture at the temperature of 28 DEG C for 12 hours, transferring to a fermentation culture medium, performing shake-flask culture at the temperature of 28 DEG C for 48 hours, and determining the content of anisic acid by a gas chromatographic method. The production rate of anisic acid obtained by optimizing the fermentation condition is 28.75 percent.

Description

technical field [0001] The present invention relates to the field of microorganism application. Specifically, it is a method for transforming anethole to produce anisic acid strains and fermenting them to produce anisic acid, Background technique [0002] Anethole is the main component of fennel oil. High value-added anisaldehyde, anisic acid, anisyl alcohol and their derivatives can be produced through deep processing. Anethole and other related compounds are widely used in food, cosmetics, spices, medicine and chemical industry and other fields. Anisaldehyde and anisic acid are widely used in the synthesis of flavors and fragrances, brighteners for electroplating metals, and are also intermediates in the synthesis of various western medicines, with high value. At present, the production of anisaldehyde and anisic acid is mainly chemical synthesis method (mainly oxidation method), which has low productivity and many by-products, which not only wastes resources but also po...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C12P7/40C12R1/01
Inventor 武波申佩弘曾华贺宋张杨蒋承建唐咸来
Owner GUANGXI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com