System and method for controlling movement of single biomolecule in nanopore

Abstract

The invention discloses a system for controlling the movement of a single biomolecule in a nanopore. The system comprises a monitor, a signal screening module, a delay module and a voltage feedback module, wherein the monitor is used for monitoring the electric characteristic values at two sides of the nanopore and sending the electric characteristic values to the signal screening module; the signal screening module is used for receiving the electric characteristic values, judging whether the single biomolecule is subjected to event of entering into or getting out of the nanopore according to the electric characteristic values and then sending a judging result to the delay module; the delay module is used for receiving the judging result and actuating corresponding movement delay according to the judging result, wherein a trigger signal can be sent to the voltage feedback module when each delay is actuated; and the voltage feedback module is used for receiving the trigger signal and outputting feedback voltage to the two sides of the nanopore according to the trigger signal so as to control the movement of the single biomolecule. By utilizing the system disclosed by the invention, the state of the single biomolecule entering into the nanopore can be correctly identified, the measurement and the control for the movement of the single biomolecule can be completed, the repeated measurement of a single basic group in DNA (Deoxyribose Nucleic Acid) sequencing is facilitated, and thus an SNR (Signal to Noise Ratio) can be increased.

Description

technical field [0001] The invention relates to the field of biology, in particular to a system and method for controlling the movement of a single biomolecule in a nanopore. Background technique [0002] Biomacromolecules, such as DNA molecules and protein molecules, are of great significance in human medicine and biology. Their structure determination and property characterization have always been international research hotspots, and they have a huge potential market in the medical and health industry. For example, under the impetus of the Human Genome Project, great progress has been made in the determination of DNA sequences. There are already many methods to characterize its structure and properties, and industrialization has gradually begun. At present, many countries in the world have invested a lot of resources in the research and development of third-generation gene sequencers, in order to lead the development of this field. [0003] Using nanopores to determine th...

AI Technical Summary

Problems solved by technology

However, the current research methods have not specifically realized the system and method for controlling the movement of individual biomolecules in nanopores, so as to control specific behaviors such as driving, pausing, continuing to move, reverse moving, and stopping.

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