Compensation method for transient current in nanopore and compensation circuit
A technology of transient current and compensation method, applied in the biological field, can solve problems such as being unable to be used by equipment, the amplitude of transient current is large, and the change of driving voltage cannot be triggered.
Inactive Publication Date: 2015-02-04
INST OF PHYSICS - CHINESE ACAD OF SCI
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[0006] However, this idea encountered a serious problem in the experimental implementation: due to the capacitance of the nanopore itself, there will inevitably be a transient capacitance charging and discharging process at the moment of the driving voltage change.
At the same time, the magnitude of this transient current is large and will generally saturate the data acquisition equipment
If the signal returned by the DNA molecule falls into this saturation region, it will not be recorded by the device, thus failing to trigger the next change in the driving voltage
Therefore, although there are thousands of continuous DNA table tennis experiment results reported, no one can shorten the delay time to below milliseconds and realize continuous table tennis motion control.
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[0017] In general, the present invention provides a method and circuit design for compensating transient currents in nanopores to precisely manipulate the motion of individual biomolecules. The invention is especially suitable for the motion control and determination of single chain molecules such as DNA and protein molecules.
[0018] figure 1 A schematic diagram of the structure of a commonly used solid-state nanopore is shown. From figure 1 It can be seen that the general nanopore structure is a hole of a few nanometers on the thin film material with suspended SiN or a small number of atomic layers on the Si sheet. In order to reduce the noise of nanopore capacitive coupling, a macromolecule insulating layer such as polydimethylsiloxane (PDMS) is applied near the hole to reduce the overall capacitance of the chip. Such a chip with nanopores is added in a microfluidic chamber with a reservoir. Since the KCl plasma solution is placed in the reservoirs on both sides of the...
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The invention discloses a compensation method for transient current in a nanopore. The method can completely compensate the transient current in the nanopore and realize precise control of biological single-molecule motion, thus contributing to repeated measurement of single basic group in DNA sequencing. According to the compensation method for the transient current in the nanopore, a driving voltage interface and a ground point are disposed at both ends of the nanopore to generate driving voltage, which is used for driving the motion of a biological single molecule passing through the nanopore to generate ionic current change, and capacitance exists in the nanopore itself, thereby generating transient current. The method successively changes the waveform of driving voltage change to compensate part of the transient current till the all transient current is compensated. The invention also provides a compensation circuit.
Description
technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a compensation method for transient current in a nanopore and a compensation circuit for realizing the method. Background technique [0002] The concept of solid-state nanopores has attracted widespread interest since it was proposed. It is widely used in biological detection because of its easy processing, simple structure, size matching with various biomolecules, and long-term stability in complex solution environments. [0003] A general nanopore is a tough silicon nitride (SiN) film with a thickness of about 40 nm prepared by chemical vapor deposition (LPCVD) on a Si sheet with a thickness of about 500 μm. A pyramidal window is etched using chemical anisotropy on one side, leaving a free SiN film at the bottom of the window. Or on a silicon substrate with a pyramid window, transfer a small amount of thin-film materials with atomic layers, such as graphene, boron nit...
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Login to View More IPC IPC(8): C12M1/34
CPCG01N33/48721
Inventor 胡颖周智单欣岩陆兴华
Owner INST OF PHYSICS - CHINESE ACAD OF SCI