Facultative anaerobic denitrifying bacteria having complete denitrification enzyme systems and use thereof

A technology of denitrifying bacteria and nitrifying enzymes is applied in the field of facultative anaerobic denitrifying bacteria, which can solve the problems of inability to remove nitrogen, and achieve the effects of saving operating costs, thorough denitrification, and high denitrification activity.

Active Publication Date: 2013-01-30
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The nitrification process is mainly to remove ammonia nitrogen. Although it can convert ammonia nitrogen into nitrate, it cannot completely remove nitrogen from the water body; the denitrification process can fundamentally eliminate the pollution of nitrogen in the water body to the environment.

Method used

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  • Facultative anaerobic denitrifying bacteria having complete denitrification enzyme systems and use thereof
  • Facultative anaerobic denitrifying bacteria having complete denitrification enzyme systems and use thereof
  • Facultative anaerobic denitrifying bacteria having complete denitrification enzyme systems and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1 Isolation and identification of bacterial strains of the present invention:

[0037] (1) Medium:

[0038] A. Strain isolation, purification, preservation medium ( / L):

[0039] CH 3 COONa, 2g; Peptone, 15g; Yeast extract, 3g; Glucose, 1g; NaCl, 6g; Agar, 12g; KNO 3 , 1.5g; the pH is controlled at 7.0-7.2.

[0040] B. Strain screening, denitrifying medium (DM: Denitrifying Medium) ( / L):

[0041] CH 3 COONa, 2g; KH2 PO 4 , 0.4g; MgSO 4 ·7H 2 O, 0.6g; CaCl 2 2H 2 O, 0.07g; KNO 3 , 1g; Tris buffer 12mL; trace elements 2mL; pH controlled at 7.0-7.2.

[0042] C. LB medium ( / L):

[0043] Peptone, 10g; yeast extract, 5g; sodium chloride, 10g; pH is controlled at 7.5.

[0044] (2) Isolation and purification of Pseudomonas XP-2 strain:

[0045] Weigh 10g of rhizosphere soil samples of cattail plants and place them in a 250mL Erlenmeyer flask, add 100mL of sterile water and a few glass beads, and vibrate on a shaker for 15min to disperse the soil samples ...

Embodiment 2

[0064] Embodiment 2 The cultivation of bacterial strain of the present invention

[0065] (1) Medium used

[0066] A. Strain preservation medium ( / L): peptone, 5g; yeast extract, 3g; glucose, 1g; NaCl, 6g; agar, 12g; KNO 3 , 1.5g; the pH is controlled at 7.0-7.2.

[0067] B. Strain denitrification medium (DM: Denitrifying Medium) ( / L): CH3COONa, 2g; KH 2 PO 4 , 0.4g; MgSO 4 ·7H 2 O, 0.6g; CaCl 2 2H 2 O, 0.07g; KNO 3 , 1g; Tris buffer 12mL; trace elements 2mL; pH controlled at 7.0-7.2.

[0068] The above medium was sterilized at 121°C for 20 minutes before use.

[0069] (2) Culture conditions

[0070] Use an inoculation loop to scrape 1 ring of bacterial lawn from the Pseudomonas XP-2 preserved on the slant of the preservation medium, inoculate it into a 250mL Erlenmeyer flask containing 100mL of sterilized LB medium, and cultivate it with constant temperature and shaking at 30°C After 12 hours, the seed liquid can be obtained. During the experiment, the seed liquid...

Embodiment 3

[0071] Example 3 The optimal denitrification conditions of the bacterial strain of the present invention

[0072] Put 100mL of sterilized denitrification medium into a 250mL Erlenmeyer flask, insert 10% of the inoculum into the seed bacterial solution, and let it stand for cultivation. In the range of 10-30°C, Pseudomonas XP-2 can remove more than 80% of total nitrogen, and the removal rate of total nitrogen is the highest at 25°C, reaching 95%. The adaptable pH value of the bacterial strain of the invention is between 6 and 9, can reduce the total nitrogen of about 140 mg / L to below 10 mg / L, and has no accumulation of nitrite. The optimum C / N (mol / mol) ratio of the strain of the invention is 3, and when C / N≥3, the removal rate of total nitrogen can reach over 90%. The slightly dissolved oxygen environment of static culture is most suitable for the denitrification activity of the strain of the present invention.

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Abstract

The invention discloses facultative anaerobic denitrifying bacteria having complete denitrification enzyme systems and a use thereof. The facultative anaerobic denitrifying bacteria are named as Pseudomonassp. XP-2, belong to pseudomonas and have a preservation number of CCTCC M 2011431. The optimal denitrification temperature of the facultative anaerobic denitrifying bacteria is in a range of 10 to 30 DEG C. In a pH value of 6-9, the facultative anaerobic denitrifying bacteria have a high-efficiency denitrification activity. When a ratio of C / N (mol / mol) is more than 3, a denitrification rate of the facultative anaerobic denitrifying bacteria is more than 90%. In an oxygen micro-dissolution environment, the facultative anaerobic denitrifying bacteria have the highest denitrification activity. Denitrification end product gas is nitrogen and thus it is proved that the facultative anaerobic denitrifying bacteria have a complete denitrification capability, and can directly reduce inorganic nitrogen substances such as nitrates and nitrites in water into harmless nitrogen, wherein the harmless nitrogen is discharged from the water. The facultative anaerobic denitrifying bacteria have a wide carbon source spectrum and can utilize a plurality of carbon sources. A performance test of the facultative anaerobic denitrifying bacteria proves that a nitrogen-containing waste water treatment technology adopting the facultative anaerobic denitrifying bacteria has simple processes and good and stable treatment effects, saves an operation cost, does not produce greenhouse gas such as No and N2O, and does not cause air pollution.

Description

technical field [0001] The invention relates to a facultative anaerobic denitrification bacterium with a complete denitrification enzyme system and its application, belonging to the field of environmental microorganisms. Background technique [0002] With the development of industrial and agricultural production and the improvement of people's living standards, the discharge of nitrogen-containing pollutants in my country has increased rapidly, large and small water bodies have become "shelters" for these pollutants, and the quality of water bodies has deteriorated sharply. At present, a large number of wastewater biological treatment projects have been established all over the country, effectively curbing the continuous deterioration of water pollution. However, the nitrogen content of the discharged water after the secondary treatment of sewage is still high, which seems to be a serious source of environmental pollution. Ammonia nitrogen and nitrate nitrogen are the main ...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): C12N1/20C02F3/34C02F3/28C12R1/38C02F101/16
Inventor裴海燕胡文容纪雁
OwnerSHANDONG UNIV