Saccharomyces ergosterol high-producing strain, and breeding method and application for same

A technology of ergosterol and high-yield strains, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of many chemical synthesis steps, difficult industrial production, high cost, etc., and achieve mild cultivation conditions and simple operation , the effect of less investment

Inactive Publication Date: 2013-02-06
SOUTH CHINA UNIV OF TECH
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Problems solved by technology

Due to the presence of asymmetric centers in the steroid molecule, many chemical synthesis steps, low yield and high cost, it is difficult to produce industrially. At present, it is mainly produced by microbial fermentation at home and abroad.

Method used

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  • Saccharomyces ergosterol high-producing strain, and breeding method and application for same
  • Saccharomyces ergosterol high-producing strain, and breeding method and application for same
  • Saccharomyces ergosterol high-producing strain, and breeding method and application for same

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Embodiment 1

[0031] (1) Preliminary screening of yeast strains tolerant to highly polar organic solvents

[0032] Insert the starting strain Saccharomyces cerevisiae (Saccharomyces cerevisiae, AS2.599, purchased from the Institute of Microbiology, Chinese Academy of Sciences) into the seed medium (add 100mL of water per 13g of wort medium, the pH is natural, the same below), and cultivate at 28°C for 18-24h Then transfer 10% by volume to the fermentation medium (add 100mL of water for every 13g of wort medium, the pH is natural, the same below), culture at 28°C for 24 hours, then draw 1.0mL of bacterial suspension into a 1.5mL sterile small centrifuge tube Add dimethyl sulfoxide, the final concentration of dimethyl sulfoxide is 12% by volume, force stress treatment for 6 hours, shake the culture on the shaker and spread the solid medium (100mL of water per 13g wort medium , agar 2g, pH natural, the same below) plate. After the colonies grow out, select colonies with good growth and large ...

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Abstract

The invention discloses a saccharomyces ergosterol high-producing strain, and a breeding method and application for the same. The saccharomyces ergosterol high-producing strain is named as saccharomyces cerevisiae scut-B6305 and is preserved in the China Center for Type Culture Collection in Wuhan, China, the preservation date is April 10, 2012, and the preservation number is CCTCC (China Center for Type Culture Collection) NO:M2012105. A saccharomyces lysate producing strain is continuously subjected to stress oriented domestication by dimethyl sulfoxide for a long time so as to breed the saccharomyces ergosterol high-producing strain. The content of ergosterol in unit cell of the saccharomyces ergosterol high-producing strain reaches 5.63mg/g and is increased by 30.32% as compared with the original strain, the production capacity of unit volume of fermentation liquor of the saccharomyces ergosterol high-producing strain is 90.8mg/100mL and is 1.91 times of that of unit volume of fermentation liquor of the original strain, and the saccharomyces ergosterol high-producing strain can be used for obtaining the ergosterol during production.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering and microbial strain breeding, and in particular relates to a yeast ergosterol high-yielding strain and a breeding method and application thereof. Background technique [0002] Ergosterol is an important pharmaceutical and chemical raw material, which can be used to manufacture VD 2 , hormone progesterone, and cortisone can effectively prevent and treat vitamin D deficiency rickets in infants and young children clinically, and they also have good preventive and therapeutic effects on fractures, bone tuberculosis, dental caries and other diseases. Due to the presence of an asymmetric center in the steroid molecule, many chemical synthesis steps, low yield and high cost, it is difficult to produce industrially. At present, microbial fermentation is mainly used for production at home and abroad. Ergosterol is found mainly in the cell membranes of plants and some fungi. Among them, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/18C12N15/01C12P33/00C12R1/865
Inventor 张毅林晓珊何小妮陈子健
Owner SOUTH CHINA UNIV OF TECH
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