Characteristics of bacteria for degrading glyphosate

A technology of glyphosate, degradation rate, applied in the field of environmental biology

Inactive Publication Date: 2020-06-19
UNIVERSITY OF CHINESE ACADEMY OF SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved in the present invention is to find a kind of bacteria that can tolerate glyphosate and red...

Method used

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  • Characteristics of bacteria for degrading glyphosate
  • Characteristics of bacteria for degrading glyphosate
  • Characteristics of bacteria for degrading glyphosate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Weigh 0.1g of aerobic bacteria and dissolve in 100ml of normal saline, shake well to make 1g / L bacterial solution. Take six 250ml Erlenmeyer flasks, add 4.6g BPDA nutrition powder and 250ml deionized water, mix well and seal. Put the prepared nutrient solution into an autoclave for sterilization. After the sterilization, when the temperature of the system is cooled to 70°C, quickly take out the Erlenmeyer flask and move it to a sterile ultra-clean workbench. Add a series of glyphosate in mass gradient to it, make the BPDA culture solution containing a series of concentration gradient glyphosate after shaking up. Pour the culture solution into a petri dish to cool down to make a BPDA solid medium containing a series of concentration gradients. Use a pipette gun to pipette 0.1ml of the bacterial solution and spread it evenly on a solid petri dish, seal the edge with a parafilm and place it in a 37°C incubator for 24 hours. bacterial growth as figure 1 Shown: In the BP...

Embodiment 2

[0019] Prepare 6 parts of BPDA liquid medium with a volume of 100ml at room temperature, and the concentration of glyphosate added to each part is 2g / L. After adjusting the pH values ​​to 5.0, 5.5, 6.0, 6.5, 7.0, and 7.5, add bacteria The concentration is 1g / L. After cultivating in a shaker at 120 rpm at 37°C for 24 hours, the bacterial liquid was centrifuged and dried, and the dry weight of the bacteria was weighed. The results obtained are shown in Table 1 and figure 2 Shown: within a certain range, with the increase of pH value, the bacterial growth first increases and then decreases. When the pH value is 5.5, the bacterial growth reaches the maximum value.

[0020] The influence of table 1 pH value on bacterial growth

[0021]

Embodiment 3

[0023] Prepare 10 parts of BPDA liquid medium with a volume of 100ml at room temperature, divide each group into two groups of A and B with 5 parts, the concentration of glyphosate added in A is 2g / L, and group B does not add glyphosate as Blank control group. After adjusting its pH value to 5.0, 5.5, 6.0, 6.5, 7.0 respectively, the concentration of bacteria added was 1g / L. After culturing in a shaker at 120 rpm at 37°C for 24 hours, the remaining amount of glyphosate was measured by the national standard method, and then the degradation rate of glyphosate by bacteria was obtained. The results obtained are shown in Table 2 and image 3 Shown: within a certain range, as the pH value increases, the degradation of glyphosate first increases and then decreases, and the degradation rate reaches the maximum value of 77% when the pH is 5.5.

[0024] The influence of table 2pH value on bacterial degradation rate

[0025] 5 5.5 6 6.5 7 Degradation rate 46％ 77％ ...

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Abstract

The invention discloses characteristics of bacteria for degrading glyphosate degrading. According to the invention, the tolerance of the bacteria to the glyphosate in a certain concentration range isshown; the conditions that the growth amount of the bacteria is increased along with the increase of a pH value and the degradation rate of the bacteria to the glyphosate is changed within a certain range are described; meanwhile, the type of the bacteria is identified to be a Bacillus cereus strain through a 16S rDNA sequencing method. The Bacillus cereus strain can effectively degrade the glyphosate in the environment under mild conditions, so the Bacillus cereus strain has high application value for repairing soil polluted by the glyphosate.

Description

technical field [0001] The invention belongs to the technical field of environmental biology and specifically studies the characteristics of a glyphosate-degrading bacterium. Background technique [0002] my country is one of the countries that use the largest amount of chemical fertilizers and pesticides in the world. Long-term use of chemical fertilizers and pesticides in large quantities will cause soil compaction, a large number of microorganisms will die, and pesticide residues in grain will also lead to a decline in its quality. Therefore, we should find ways to reduce the use of chemical fertilizers and pesticides when we have to use them, and improve and restore the polluted soil to increase the organic matter and microbial content in the soil. However, the existing pesticides in the soil make some beneficial microorganisms unable to survive, so we must find ways to degrade the pesticides in the soil. One of the most widely used herbicides in pesticides is glyphosa...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/085
CPCB09C1/10B09C2101/00C12N1/20C12N1/205C12R2001/085
Inventor 何裕建闫玉吴丽
Owner UNIVERSITY OF CHINESE ACADEMY OF SCIENCES
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