Bacterial strain capable of degrading high molecular polycyclic aromatic hydrocarbons and application of bacterial strain in contaminated soil bioremediation
A polycyclic aromatic hydrocarbon and polymer technology, which is applied in the fields of environmental engineering and microbial engineering, can solve the problems of repairing polycyclic aromatic hydrocarbons without Rhizobia, and achieve the effects of wide range, rapid degradation ability and high degradation rate.
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Embodiment 1
[0035] The separation and screening method of tzym provided by the invention.
[0036] Collect surface soil samples 5-20cm below the surface from the solid waste dismantling site, and use them as bacteria sources for natural enrichment culture. Glucose 2g, tryptone 4g, beef extract 4g, MgSO 4 0.8g was added to 400mL of distilled water, the prepared culture solution was sterilized at 121°C for 20 minutes, cultivated on this medium for three days, and prepared inorganic salt basic medium, adding pyrene, benzo[a ] anthracene, benzo [b] fluoranthene, benzo [a] pyrene, dibenzo [a, b] anthracene polycyclic aromatic hydrocarbon compound, add distilled water to settle to 500mL after being prepared into a solution, add pyrene, benzene The amount of a[a]anthracene, benzo[b]fluoranthene, benzo[a]pyrene, and dibenzo[a,b]anthracene should make pyrene, benzo[a]anthracene, and benzo[a]anthracene in the prepared culture solution The final concentrations of a[b]fluoranthene, benzo[a]pyrene, ...
Embodiment 2
[0039] Degradation of high-molecular polycyclic aromatic hydrocarbons by A. tumefaciens.
[0040] Select pyrene, benzo [a] anthracene, benzo [b] fluoranthene, and benzo [a] pyrene with concentrations of 200 mg / Kg and 100 g of contaminated soil polluted by polycyclic aromatic hydrocarbons, inoculate tzym, and the addition amount is 3.5% of the dry weight of the soil. %, add a small amount of water to keep the moisture content of the soil at about 15%, put it into a shaker, control the culture temperature at a constant temperature of 20°C, shake the culture and restore it for 120 hours, and perform liquid chromatography analysis after extraction and purification. The degradation rates of benzo[a]anthracene, benzo[b]fluoranthene and benzo[a]pyrene are all above 90%.
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