Unlock instant, AI-driven research and patent intelligence for your innovation.

Chemical method for detecting 5-methylcystein in DNA

A technology of methylcytosine, chemical method, applied in the direction of biochemical equipment and methods, determination/inspection of microorganisms, etc.

Inactive Publication Date: 2014-03-12
WUHAN UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, the accuracy of these methods for detecting 5-methylcytosine needs to be further improved

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Chemical method for detecting 5-methylcystein in DNA
  • Chemical method for detecting 5-methylcystein in DNA
  • Chemical method for detecting 5-methylcystein in DNA

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The present invention uses the DNA sequence fragments of the promoter regions in gastric cancer and cervical cancer to analyze, react with p-nitrobenzenesulfonyl chloride sodium salt and m-nitrobenzenesulfonyl bromide sodium salt, and then process it with piperidine , you can clearly see the position of 5-methylcytosine in the DNA sequence.

[0034] 1) Preparation of p-nitrobenzenesulfonyl chloride sodium salt

[0035] Add 0.8 g of solid sodium hydroxide and 2 mmol of p-nitrobenzenesulfonamide to 2 ml of aqueous solution, stir for 5 min, then add 3 ml of sodium hypochlorite solution, stir at room temperature for 12 h, filter, wash the resulting solid with cold water 5 times, and then weigh it with water. Crystallization gave pale yellow crystals. The yield was 83%. 1 H-NMR (300MHz, d 6 -DMSO)δ=8.27(d,J=7.5H z ,2H),7.88(d,J=7.5H z ,2H). 13 C-NMR (75MHz, d 6 -DMSO) δ=156.72, 153.18, 133.55, 128.80.

[0036] 2) Preparation of m-nitrobenzenesulfonyl bromide sodium s...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
volumeaaaaaaaaaa
volumeaaaaaaaaaa
Login to View More

Abstract

The invention relates to a chemical method for detecting 5-methylcystein in DNA (Deoxyribose Nucleic Acid). Two compounds adopted in the method are substituted chloramine-B hydrate and substituted bromoamine-B hydrate. The method comprises the detection steps of allowing the substituted chloramine-B hydrate to react with cytosine in the fluorescently-labeled DNA, then allowing the substituted bromoamine-B hydrate to react with the cytosine and the 5-methylcystein in the fluorescently-labeled DNA, treating with piperidine, and comparing two reacted DNA zones in DNA polyacrylamide denatured gel to clearly observe the positions and the number of the 5-methylcystein.

Description

technical field [0001] The invention relates to a chemical detection method for 5-methylcytosine in DNA for non-disease diagnosis. Background technique [0002] Epigenetics is defined as the study of the inheritance of information on gene expression levels (quantitative changes). DNA methylation is a major form of epigenetics. [0003] DNA methylation has a great relationship with tumors. In 2003, three articles published in the same issue of the American "Science" magazine have affirmed that the gene instability caused by the overall hypomethylation of DNA plays a role in the occurrence of tumors. In mouse models, it has been demonstrated that mutations in DNA methyltransferases (DNMTs) lead to chromosomal demethylation during embryonic to adult development, causing genome instability and lymphoma in mice. Generally speaking. The overall DNA methylation level of tumor cells is lower than that of normal cells, but the CpG islands of some specific genes (such as tumor sup...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 周翔王天露翁小成
Owner WUHAN UNIV