Method for extracting total flavone from guava leaves
A technology for guava leaves and total flavonoids, which is applied in the field of extracting total flavonoids, can solve the problems of high cost and low content of total flavonoids, and achieves the effects of low cost, simple method and high efficiency
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specific Embodiment approach 1
[0016] Specific embodiment one: a kind of method extracting total flavonoids from guava leaf in the present embodiment is carried out according to the following steps:
[0017] 1. Take 50-60kg of guava leaves, decoct them with water for at least 2 times, filter, combine the decoction liquid, and concentrate at a temperature of 60-80°C until the relative density is 1.12-1.14g / cm 3 The clear paste; wherein the first decocting water consumption is 14 to 20 times the guava leaf quality, the second decoction water consumption is 10 to 14 times the guava leaf quality, each decoction water consumption Decrease by 20% to 30% in turn, and each decoction takes 1 to 3 hours;
[0018] 2. Add water to the clear paste obtained in step 1 until the volume is 150-200L, then adjust the pH value to 6.0-7.0 with 2-4mol / L sodium hydroxide solution, soak for 12-24 hours, collect the supernatant, and use 4 ~5mol / L hydrochloric acid solution to adjust the pH value of the supernatant to 2.0~3.0, let ...
Embodiment approach
[0022] This embodiment includes the following effects:
[0023] 1. This embodiment adopts the polyamide purification method. The polyamide resin is a kind of polymer material formed by the polymerization of amides. It has strong adsorption properties for flavonoids, mainly through hydrogen bonds and van der Waals forces. In this embodiment, only low-cost eluents such as ethanol and acetone are used, so the cost is low, and the method is simple, high in efficiency, good in stability and easy to regenerate, and the obtained product is safe and free of heavy metal and organic solvent toxicity problems such as residues;
[0024] 2. According to the pharmaceutical method, the total flavonoids of the effective parts of guava leaves in this embodiment can be made into various pharmaceutical preparations suitable for clinical use. Various dosage forms include oral formulations as well as parenteral formulations. The oral preparations can be made into granules, tablets, capsules, pil...
specific Embodiment approach 2
[0026] Embodiment 2: The difference between this embodiment and Embodiment 1 is that each decoction in step 1 takes 1.5 hours. Others are the same as in the first embodiment.
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