Cell culture insert

A cell culture and planar technology, which is applied in the field of cell culture containers and cell culture pools, can solve the problems of unobtainable, limited cell biology research, and inability to study biological behavior, etc., and achieve a simple structure and easy mass production Effect

Inactive Publication Date: 2013-02-13
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when it is necessary to study the interaction between two different types of cells, if the two types of cells to be studied are directly inoculated into a common flat-bottomed cell culture vessel, the two types of cells will be mixed with each other and evenly distributed In the culture container, researchers can only obtain the situation when two different types of cells are mixed with each other and grow, but cannot obtain two k

Method used

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Examples

Experimental program
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Example Embodiment

[0023] Example 1

[0024] in figure 1 In the shown embodiment 1, the cell culture tank is designed as a cylindrical container, and the material is colorless, high-transparency, crystal-grade polystyrene. The inner diameter of the container is 30mm, the height of side wall 1 is 16mm, and the thickness of side wall 1 is 1mm. It can be placed in an ordinary six-well cell culture plate. On the top end of the side wall 1, there are three lateral wings 3 that extend in parallel, and the lateral wings are 2mm long, 2mm wide and 1mm thick. The bottom wall 2 of the container has a thickness of 1 mm. The bottom wall 2 is composed of a platform part 4 located in the middle of the bottom wall 2 and an arc surface 5 and a slope-like flat surface 6 located on both sides of the platform part 4. The platform part 4 is a horizontal plane with a width of 3 mm, and its long axis direction is connected with the side wall 1. An arc surface 5 is provided between the platform portion 4 and the slope-...

Example Embodiment

[0027] Example 2

[0028] in Image 6 In the shown embodiment 2, the cell culture tank is designed as a regular quadrangular prism-shaped container, and the material is colorless and transparent glass. The square prism-shaped side wall 1 has a side length of 20mm, the side wall 1 has a height of 10mm, and the side wall 1 has a thickness of 1mm, which can be placed in an ordinary six-well cell culture plate. At the four corners of the top end of the side wall 1, there are four lateral wings 3 extending parallel to each other. The lateral wings 3 are 2mm long, 2mm wide and 1mm thick. The bottom wall 2 of the container has a thickness of 1 mm. The bottom wall 2 is composed of a platform part 4 located in the middle of the bottom wall 2 and an arc surface 5 and a slope-like flat surface 6 located on both sides of the platform part 4. The platform part 4 is a rectangular horizontal plane with a width of 3mm and a length of 20mm. An arc surface 5 is provided between the platform port...

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Abstract

The invention relates to a cell culture insert, wherein two different types of cells can separately and independently grow into cell populations in the same container, and then the two cell populations grow to contact each other to form a cell junction area through the cell culture insert. The cell culture insert is a transparent container, and comprises a side wall (1) and a bottom wall (2), wherein the top end of the side wall (1) is distributed with a plurality of outwardly extending side wings (3), and the bottom wall (2) comprises a platform portion (4) positioned on the middle of the bottom wall (2), arc surfaces (5) positioned on both sides of the platform portion (4), and slope-like planes (6). Two types of cells are respectively inoculated onto both sides of the platform portion (4) by a researcher, a liquid level of a culture medium is lower than the platform portion (4), the two types of the cells can grow in an isolated manner, after the cells adhere to the wall to form cell populations, the culture medium is added to the liquid level of higher than the platform portion (4), the two types of the cells grow and extend toward the dome plane, when the two types of the cells contact each other, biological behavior conditions of the two types of the cells on the dome plane cell junction area can be observed through a microscope when the two types of the cells interact.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a culture container used for culturing cells in biological science research. In particular, two different cells can be isolated and cultured in the same container to make them adhere to and grow separately to form a cell group, and then make the two cell groups Continue to grow until they touch each other and form a cell culture pool with a clear cell junction area. Background technique [0002] At present, the bottoms of cell culture containers such as culture flasks, culture plates, culture tanks, and culture dishes used to culture cells in biological science research are all flat. When researchers inoculate a certain type of cell in the culture container, the inoculated cells It is injected into the culture container together with the liquid culture medium. The cells are suspended in the culture medium. Therefore, the cells inoculated into the culture container will be evenl...

Claims

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Application Information

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IPC IPC(8): C12M3/04
Inventor 李挥力王国斌陶凯雄王继亮黄坤
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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