Staurosporine preparation method

A technology for staurosporine and Streptomyces scabies, which is applied in the field of preparation of staurosporine by using a fermentation method

Active Publication Date: 2013-02-13
安阳市喜满地肥业有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is no relevant report on the research of Streptomyces as a biocontrol strain in China. Therefore, it is of great significance to study the optimization of fermentation conditions and the separation and purification of metabolites using Streptomyces as a biocontrol strain.

Method used

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Examples

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Embodiment Construction

[0036] The experimental methods used in the following examples are conventional methods unless otherwise specified.

[0037] Main Reagents and Instruments

[0038] Strain DNA extraction kit was purchased from Tiangen Biochemical Technology (Beijing) Co., Ltd.; gel recovery kit, plasmid extraction kit and Maker were purchased from Shanghai Laifeng Biotechnology Co., Ltd.; agarose and beef extract from Oxoid, UK ; 3K30 high-speed centrifuge from Sigma Company in the United States; T-gradient PCR instrument from Biometra Company in Germany; GelDoc-IT gel imaging system from UVP Company in the United States; DYY-8B electrophoresis instrument and DYY- Type III electrophoresis tank; Japan OLYMPUS company B202 multifunctional optical microscope; Japan Hitachi company S-3400NII scanning electron microscope; KP-48 three-purpose quartz ultraviolet detector; SPD10101 centrifugal concentrator; RE-5000 rotary evaporator ; Hitachi UV-VIS3010 UV-Vis spectrophotometer; Shimadzu Analytical HP...

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Abstract

The present invention relates to a staurosporine preparation method, wherein a molecular formula of the staurosporine is C28H26N4O3, and the staurosporine is prepared through streptomyces scabiei var.xuchangensis CGMCC No.5213 fermentation. According to the present invention, streptomyces scabies fermentation is adopted to produce the staurosporine so as to provide a new way for staurosporine production; and the staurosporine produced from the streptomyces scabiei var.xuchangensis provides strong antagonism for a plurality of plant pathogenic fungi, provides a strong in vitro deactivation effect for TMV, and has development potential as an agricultural antibiotic.

Description

technical field [0001] The invention belongs to the technical field of compound synthesis, and mainly relates to a method for preparing staurosporine by using a fermentation method. Background technique [0002] Pesticide is an important and indispensable material to solve the world's food problem and ensure stable and high yield of crops. It is also an industry that can obtain huge economic and social benefits. However, problems such as environmental pollution, human and animal poisoning, and increased resistance of diseases and insect pests caused by chemical pesticide residues are becoming more and more serious. Vigorous research, development, production and application of biological pesticides have become a new trend in the development of the global agricultural industry. At present, countries around the world are taking practical steps to reduce the use of chemical pesticides. For example, the US Environmental Protection Agency has announced the cancellation of the regi...

Claims

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Application Information

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IPC IPC(8): C12P17/18C07D498/22C12R1/465
Inventor 文才艺
Owner 安阳市喜满地肥业有限责任公司
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