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Phencyclidine detection kit and preparation method thereof

A detection kit, the technology of phencyclidine, which is applied in the field of biological immunoassay determination, can solve the problems of high requirements on expensive instruments and equipment and detection materials, and achieve the effects of rapid response, simple operation and convenient use.

Active Publication Date: 2015-11-25
宝瑞源生物技术(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There are many detection methods related to phencyclidine, such as methods such as high performance liquid chromatography, gas chromatography, gas chromatography-mass spectrometry, although these methods have high detection sensitivity and accurate detection results, but they require expensive Instruments and equipment have high requirements for testing materials, and limitations such as purification are required

Method used

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  • Phencyclidine detection kit and preparation method thereof
  • Phencyclidine detection kit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1: the preparation of phencyclidine detection kit

[0036] 1. Preparation of phencyclidine-conjugated antigen

[0037] The phencyclidine is coupled with BSA to synthesize the phencyclidine-BSA coupled antigen as the phencyclidine coupled antigen.

[0038] 2. Preparation of phencyclidine monoclonal antibody

[0039] Using phencyclidine-BSA conjugated antigen as immunogen to immunize BALB / C mice, through hybridoma technology, a hybridoma cell line secreting anti-phencyclidine monoclonal antibody was obtained; a large amount of antibody was prepared by in vivo induced ascites method, Use ProteinG column to carry out purification, obtain phencyclidine monoclonal antibody.

[0040] 3. Preparation of colloidal gold

[0041] Take 90ml of purified water in a clean round bottom flask, stir and heat in the electromagnetic heating mantle, after boiling, add 4ml of 1% gold chloride solution, continue stirring for 1min, quickly add 6ml of 1% trisodium citrate, the solu...

Embodiment 2

[0051] Embodiment 2: the detection of phencyclidine detection kit

[0052] 1. Detection method:

[0053] Take out the phencyclidine detection kit and place it horizontally; drop 3 drops of sample on the sample pad, observe and record the color development of C and T lines after 10 minutes, and judge the test result.

[0054] 2. Result judgment

[0055] Positive: T line does not show color, only C line shows color, it is judged as a positive result;

[0056] Negative: Both the T line and the C line are colored, and it is judged as a negative result;

[0057] Invalid: The C line does not develop color, indicating incorrect operation or the kit has deteriorated and damaged.

[0058] When detecting the sample, the sample is chromatographed toward one end of the suction pad due to capillary action. If the tested sample contains phencyclidine, they will compete with the phencyclidine-BSA conjugated antigen coated on the detection line (T line) for limited antibody binding on the...

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Abstract

The invention relates to a phencyclidine detection kit, which comprises a sample pad (1), a colloidal gold pad (2), a nitrocellulose membrane (3), a sample suction pad (4) and a PVC support plate (5), wherein the PVC support plate is sequentially and successively adhered with the sample pad, the colloidal gold pad, the nitrocellulose membrane and the sample suction pad, the colloidal gold pad is a colloidal gold-labeled phencyclidine monoclonal antibody polyester film, and the nitrocellulose membrane is sequentially coated with phencyclidine-BSA conjugated antigen as a detection line (T line) and goat anti-mouse IgG polyclonal antibody as a quality control line (C line). According to the present invention, a colloidal gold immunochromatography assay technology is adopted to prepare the phencyclidine detection kit for detecting the phencyclidine possibly existing in a sample, and the kit has characteristics of simple preparation method, easy use, quick reaction, economy, practicality and the like.

Description

technical field [0001] The invention relates to the technical field of determination of biological immunological methods, in particular to a phencyclidine detection kit made by colloidal gold immunochromatography technology and a preparation method thereof. Background technique [0002] Phencyclidine is a psychoactive drug with depressant, excitatory, analgesic and hallucinogenic effects on the central nervous system. Often results in an acute syndrome of disorientation, agitation, and delirium. Agents that produce similar effects are dextrobenzoxidine and ketamine. Illegal use of PCP is generally smoke inhalation (orally, intravenously). It starts to work within 5 minutes after taking the medicine, and reaches the peak in about 30 minutes, feeling euphoria, warmth, tingling, floating sensation and tranquility isolated from the outside world. Visual and auditory hallucinations may also occur, as well as body image changes, distortions in space-time perception, delusions, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577
Inventor 陈立柱杨利
Owner 宝瑞源生物技术(北京)有限公司