Lactobacillus bulgaricus freeze-drying protecting agent and preparation method thereof

A freeze-drying protective agent, the technology of Lactobacillus, applied in the field of preservation of Lactobacillus bulgaricus, can solve problems such as unfavorable production, cell death or damage, and achieve the effects of low cost, improved anti-freezing ability, and low survival rate

Active Publication Date: 2013-03-06
SHAANXI YATAI DAIRY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the preparation of Lactobacillus bulgaricus powder mainly adopts vacuum freeze-drying technology, but if Lactobacillus bulgaricus is directly freeze-dried, it will inevit...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The Lactobacillus bulgaricus lyoprotectant consists of: 22g of lactose, 26g of skim milk powder, 4.6g of sodium ascorbate, 40000.3g of polyethylene glycol, 0.4g of polyvinylpyrrolidone PVP, 0.7g of bovine serum albumin BSA, and 100ml of distilled water.

[0031] Its preparation method:

[0032] 1) Heat and dissolve lactose and skim milk powder according to the amount, mix well, and adjust the pH to 6.9 with NaOH solution, then sterilize at 110°C for 15 minutes, and cool to room temperature;

[0033] 2) Weigh polyvinylpyrrolidone PVP according to the amount, dissolve it in distilled water, sterilize at 115°C for 30min, and cool to room temperature;

[0034] 3) Dissolve sodium ascorbate, polyethylene glycol 4000 and bovine serum albumin BSA in 0.85-0.9% sterile saline according to the amount, and filter and sterilize with a 0.22 μm filter membrane;

[0035] 4) Mix the two moist heat sterilized solutions of step 1) and 2) according to the amount, and add sodium ascorbate,...

Embodiment 2

[0038] The Lactobacillus bulgaricus freeze-drying protectant consists of: lactose 23g, skim milk powder 24g, sodium ascorbate 4.7g, polyethylene glycol 40000.3g, polyvinylpyrrolidone PVP 0.3g, bovine serum albumin BSA 0.6g, distilled water 100ml.

[0039] Its preparation method:

[0040] 1) Heat and dissolve lactose and skim milk powder according to the amount, mix well, and adjust the pH to 7.0 with NaOH solution, then sterilize at 110°C for 15 minutes, and cool to room temperature;

[0041] 2) Weigh polyvinylpyrrolidone PVP according to the amount, dissolve it in distilled water, sterilize at 115°C for 30min, and cool to room temperature;

[0042] 3) Dissolve sodium ascorbate, polyethylene glycol 4000 and bovine serum albumin BSA in 0.85-0.9% sterile saline according to the amount, and filter and sterilize with a 0.22 μm filter membrane;

[0043] 4) Mix the two moist heat sterilized solutions of step 1) and 2) according to the amount, and add sodium ascorbate, polyethylene ...

Embodiment 3

[0046] The Lactobacillus bulgaricus freeze-drying protectant consists of: lactose 26g, skim milk powder 24g, sodium ascorbate 4.8g, polyethylene glycol 40000.2g, polyvinylpyrrolidone PVP 0.5g, bovine serum albumin BSA 0.8g, distilled water 100ml.

[0047] Its preparation method:

[0048] 1) Heat and dissolve lactose and skim milk powder according to the amount, mix well, and adjust the pH to 7.1 with NaOH solution, then sterilize at 110°C for 15 minutes, and cool to room temperature;

[0049] 2) Weigh polyvinylpyrrolidone PVP according to the amount, dissolve it in distilled water, sterilize at 115°C for 30min, and cool to room temperature;

[0050] 3) Dissolve sodium ascorbate, polyethylene glycol 4000 and bovine serum albumin BSA in 0.85-0.9% sterile saline according to the amount, and filter and sterilize with a 0.22 μm filter membrane;

[0051] 4) Mix the two moist heat sterilized solutions of step 1) and 2) according to the amount, and add sodium ascorbate, polyethylene ...

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PUM

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Abstract

The invention discloses a lactobacillus bulgaricus freeze-drying protecting agent and a preparation method thereof. The lactobacillus bulgaricus freeze-drying protecting agent comprises the following components in parts by mass: 20-30 parts of lactose, 20-30 parts of dried skim milk, 3-5 parts of sodium ascorbate, 0.2-0.6 part of polyethylene glycol 4000, 0.2-0.8 part of polyvinylpyrrolidone, 0.5-2.0 parts of bovine serum albumin and 100 parts of distilled water. The freeze-drying protecting agent is added in a powder preparation process, so that the anti-freeze capacity of the thalli can be improved, thereby improving the freeze-dry survival rate of the powder and guaranteeing long storage period and low probability for reduction of the survival rate.

Description

technical field [0001] The invention belongs to the technical field of Lactobacillus bulgaricus preservation, and relates to a freeze-drying protectant for Lactobacillus bulgaricus and a preparation method thereof. Background technique [0002] Lactobacillus bulgaricus (Lactobacillus bulgaricus) is one of the most important physiological flora in the normal human intestinal tract. It has the functions of maintaining the micro-ecological balance of the human body, promoting digestion, preventing and fighting cancer, enhancing immunity, and anti-aging. It is closely related to the health of the body. [0003] At present, the preparation of Lactobacillus bulgaricus powder mainly adopts vacuum freeze-drying technology, but if Lactobacillus bulgaricus is directly freeze-dried, it will inevitably cause death or damage of some cells, which is very unfavorable to production. It has been reported that if the culture medium is directly freeze-dried, the survival rate is only about 10%...

Claims

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Application Information

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IPC IPC(8): C12N1/04C12R1/225
Inventor 陈合李串娜舒国伟王勇
Owner SHAANXI YATAI DAIRY CO LTD
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