Method for tissue culture and rapid propagation by basal disc-free scales of Lycoris chinensis

A tissue culture rapid propagation and scale technology, which is applied in horticultural methods, botanical equipment and methods, applications, etc., can solve the problems of complex seed genetic background, restrictions on the popularization and application of pure Chinese Lycoris species, separation of progeny traits, and the like. Consistent genetic basis, significant competitive advantage, and uniform growth potential

Inactive Publication Date: 2013-03-13
ZHEJIANG FORESTRY UNIVERSITY
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Problems solved by technology

[0004] CN102217540A discloses Zhou Jian's patented technology of "a method for rapid propagation of Chinese Lycoris", which uses the seed embryo of Chinese Lycoris as explants, first induces the explants to produce callus, and then induces adventitious buds and small...

Method used

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  • Method for tissue culture and rapid propagation by basal disc-free scales of Lycoris chinensis
  • Method for tissue culture and rapid propagation by basal disc-free scales of Lycoris chinensis
  • Method for tissue culture and rapid propagation by basal disc-free scales of Lycoris chinensis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: Select Chinese Lycoris bulbs with sufficient nutrition, remove external debris, soak in detergent for 10 minutes, rinse with running water for 2 hours, absorb the surface moisture and move to an aseptic operating table for disinfection; use a scalpel to cut the scales into The fragments of 1.0-1.5cm are inoculated on the culture medium; the inoculated materials are placed on the culture rack for cultivation, and after one and a half months, the differentiation of callus and small bulbs on the scales can be observed.

Embodiment 2

[0035] Embodiment 2:: select the upper half of the aseptic seedling bulblet without the base plate, remove the leaves, inoculate in the induction medium, place and cultivate under the same culture conditions as in Example 1, and it can be observed after 1 month The culture medium differentiates into calli.

Embodiment 3-5

[0036] Example 3-5: The induced callus is divided into several small pieces according to the size of 0.5×0.5cm, and transferred to the proliferation medium; the upper part of the induced bulblet is cut off, leaving the base The bottom of the dish was then transferred to proliferation medium. After a certain amount of propagation is reached, each small bulb is separated, and after the leaves are removed, it is inoculated in the strong seedling medium for 1 month to promote the growth of the bulb to a diameter of about 0.5 cm, and rooting culture can be carried out. The bulbs grow three strong fibrous roots of more than 1cm, and they can be domesticated and transplanted.

[0037] In the five examples in the above table, the success rate is above 76%. For the cultures that do not meet the requirements of the next growth stage, the culture time can be extended until a satisfactory effect is obtained.

[0038] In short, the key of this method is to grasp the selection and treatmen...

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Abstract

The invention relates to a method for tissue culture and rapid propagation by basal disc-free scales of Lycoris chinensis. The method comprises the steps of: (1) based on an MS culture medium, adding 6-BA, NAA, 2, 4-D and other plant growth regulators; (2) selecting basal disc-free Lycoris chinensis scales or aseptically cultured bulblets; (3) controlling the culture room temperature at 23-27DEG C, and conducting illumination for 1000-1200lx; (4) inducing calluses and bulblets, then conducting cutting and transferring them into a proliferation medium to carry out subculture; (5) performing strong seedling culture; (6) conducting rooting culture; and (7) disinfecting seedlings then performing transplanting, keeping a moist environment, and when seedling leaves expand, carrying out normal management. The invention makes use of basal disc-free scales of Lycoris chinensis to establish a rapid propagation system, and has the advantages of fewer materials, high propagation coefficient and the like. The obtained seedlings have consistent genetic basis, stable traits, and tidy growth potential, and have a significant competitive edge in cultivation of new ornamental and medicinal varieties.

Description

1. Technical field [0001] The invention relates to a method for rapidly propagating bulbs by using Chinese lycoris without basal scales to establish an aseptic culture system. 2. Background technology [0002] Lycoris chinensis belongs to Lycoris plant of Amaryllidaceae Amaryllidaceae. It is mainly distributed in the Yangtze River Basin and Southwest provinces in China. It is a kind of excellent bulbous flower plants and medicinal plants. At present, it has been applied in different fields such as urban greening, sales of fresh cut flowers, and medicinal use. Under natural conditions, Chinese Lycoris mainly relies on bulb division and seed self-sowing for reproduction, with a very low reproduction coefficient, and the limited number of wild resources makes it difficult to meet large-scale production applications. Therefore, the development of Chinese Lycoris tissue culture and rapid propagation technology is of great significance to the protection of wild populations and th...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 姜小凤童再康时剑高燕会
Owner ZHEJIANG FORESTRY UNIVERSITY
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