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Method for tissue culture and rapid propagation by using Lycoris chinensis leaves

A leaf group and technology of Lycoris, applied in horticultural methods, botanical equipment and methods, applications, etc., can solve the problems of restricting the popularization and application of pure varieties of Lycoris in China, complex genetic background of seeds, and segregation of offspring traits, etc. Achieve significant competitive advantages, rapid breeding, and stable traits

Inactive Publication Date: 2013-03-20
ZHEJIANG FORESTRY UNIVERSITY
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Problems solved by technology

[0004] CN102217540A discloses Zhou Jian's "a method for rapid propagation of Chinese Lycoris" invention patent technology, which uses the seed embryo of Chinese Lycoris as explants to induce regenerated plants. Although this method has a high reproductive coefficient, the genetic The background is complex, and the traits of the offspring bred are segregated, which limits the popularization and application of pure varieties of Lycoris in China

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  • Method for tissue culture and rapid propagation by using Lycoris chinensis leaves
  • Method for tissue culture and rapid propagation by using Lycoris chinensis leaves
  • Method for tissue culture and rapid propagation by using Lycoris chinensis leaves

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Embodiment 1

[0039] Example 1: The leaves of Lycoris chinensis are collected in spring from young leaves that are creamy yellow and have not yet turned green, and the sampling bag is sealed and brought back to the laboratory, rinsed carefully with clean water to remove the dirt on the surface of the leaves, and then sent to the inoculation room for sterilization and inoculation; Sterilize in the following order: sterilize in 75% ethanol for 30s-1min→wash in sterile water for 2 times→sterilize in 01% HgCl2 solution for 8-10min→wash in sterile water for 4 times→absorb the surface moisture with sterile filter paper. Ensure the tissue activity of the leaves; then use a scalpel to cut the leaf segments into 0.8-1.0cm segments, cut out the wounds around them, and inoculate them on the culture medium. The above operation process is carried out within 30 cm of the flame of the alcohol lamp; The culture bottle is placed under dark culture conditions, and the temperature is kept at 25±2°C. After 2-3 ...

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Abstract

The present invention relates to a method for tissue culture and rapid propagation by using Lycoris chinensis leaves. The method comprises the following steps: (1) adopting a MS culture medium as a base culture medium, and adding 6-BA, NAA, 2,4-D and other plant growth regulators; (2) acquiring Lycoris chinensis young leaves, and carrying out sterilization; (3) inoculating fragments of the leaves on the appropriate culture medium; (4) performing dark culture at an induction phase, and providing illumination at a secondary culture stage and a rooting culture stage; (5) carrying out secondary culture when a height of small bulb is 0.5 cm; (6) carrying out rooting culture; and (7) carrying out acclimation seedling exercising, maintaining a moist environment, and performing normal management until leaves of the seedling stretch. According to the present invention, the Lycoris chinensis leaves are utilized to establish a rapid propagation system, advantages of no damage on mother bulb and rare germplasm protection are provided, and the obtained seedlings have characteristics of uniform heredity base, stable character, tidy growth potential and the like, and have significant competitive advantages in breeding and utilization of new ornamental and medicinal varieties.

Description

1. Technical field [0001] The invention relates to a method for quickly cultivating regenerated plants by using the leaf tissue of Lycoris chinensis to establish an aseptic culture system. 2. Background technology [0002] Chinese lycoris Lycoris chinensis belongs to Amaryllidaceae Amaryllidaceae Lycoris plant, widely distributed in East Asia, mainly distributed in the Yangtze River Basin and Southwest provinces in China, known for its unique dragon-claw-shaped petals, colorful flowers, flower-shaped Elegant, is an excellent class of bulbous flower plants and medicinal plants. At present, it has been applied in urban afforestation in East China. However, under natural conditions, Chinese Lycoris mainly relies on bulb division for reproduction, and the reproduction coefficient is low, which is difficult to meet the needs of large-scale production. Due to the lag of rapid propagation technology measures, garden application and medicinal excavation have seriously affected the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 姜小凤童再康时剑高燕会
Owner ZHEJIANG FORESTRY UNIVERSITY
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