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Preparation method of granular paecilomyces lilacinus biological nematocide

A bionematicide, Paecilomyces lilacinus technology, applied in the separation of microorganisms, etc., can solve the problems of large-scale production limitations and achieve low production costs

Active Publication Date: 2014-08-20
北京市西山试验林场管理处
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods are limited to a small amount of production preparation, and large-scale production is limited

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  • Preparation method of granular paecilomyces lilacinus biological nematocide

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preparation example Construction

[0016] According to the preparation method of the present invention, the strain is a Paecilomyces lilacinus strain, which can be purchased and can also be cultivated by the following method:

[0017] Potato agar medium (PDA) is used to obtain by a method known to those skilled in the art, and the specific parameters in the culture are selected as follows: The temperature range of strain growth is 8-38°C, and the best is 25-30°C The pH of strain growth is 2-11, preferably pH 5-9. The preparation method of the culture medium is as follows: peel the potato and cut into pieces, 200g boil for 20min, filter with gauze, add 20g glucose and 15g agar powder to the filtrate, dilute the volume to 1000ml with distilled water, subpackage, and autoclave sterilization Standby after 20 minutes.

[0018] The strain can be preserved by the following method: the above-obtained strain adopts the ordinary test tube PDA medium block method, that is, cut out the well-grown bacterial block grown on the P...

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Abstract

The invention provides a preparation method of granular paecilomyces lilacinus biological nematocide, and the method comprises the following steps of: preparing strain, producing strain, carrying out primary fermentation, carrying out secondary fermentation and pelleting. In the method, a culture medium comprises cane sugar, yeast, MgSO4, KCl, ZnSO4.7H2O and distilled water. By selecting various technological conditions in the preparation method of the paecilomyces lilacinus biological nematocide, the invention provides a method for producing the paecilomyces lilacinus biological nematocide on a large scale; and the prepared paecilomyces lilacinus biological nematocide is high in robe yield and low in production cost.

Description

Technical field [0001] The invention relates to a preparation method of a biological nematicide, in particular to a preparation method of a Paecilomyces lilacinus biological nematicide, belonging to the field of preparation of Paecilomyces lilacinus biological nematicide. Background technique [0002] Among the four major pathogens that cause plant invasive diseases, plant parasitic nematodes are more harmful than bacteria and viruses, and second only to fungal diseases. Plant parasitic nematodes cause about 100 billion U.S. dollars in losses in world agricultural production each year. Meloidogyne spp. is the most harmful type of plant parasitic nematodes. The economic losses caused by only important economic crops in the world are as high as several. Ten billion dollars. In Meloidogyne, the southern root knot nematode (M.incognita), peanut root knot nematode (M.arenaria), Java root knot nematode (M.javanica) and northern root knot nematode (M.hapla) The root knot nematode dise...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/02
Inventor 梁洪柱田会鹏陈倩李学燕
Owner 北京市西山试验林场管理处