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Preparation method of lactic acid bacteria liquid for animal cultivation

A technology of lactic acid bacteria liquid and animal breeding, applied in animal feed, animal feed, application, etc., can solve the problems of difficult promotion and application of lactic acid bacteria preparations, high requirements for aseptic conditions, and large investment in equipment, so as to improve feed conversion efficiency and increase The effect of average daily gain and low production cost

Active Publication Date: 2013-03-13
北京同力兴科农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional lactic acid bacteria preparations, whether it is solid dry powder or bottled or barreled bacterial liquid, are all produced in a pure culture environment. The production process requires high aseptic conditions, large equipment investment, high energy consumption, and high-temperature sterilization of raw materials. Bacteria, the production cost per ton of liquid preparations is at least 5,000 yuan, which leads to great difficulties in the promotion and application of lactic acid bacteria preparations

Method used

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  • Preparation method of lactic acid bacteria liquid for animal cultivation
  • Preparation method of lactic acid bacteria liquid for animal cultivation
  • Preparation method of lactic acid bacteria liquid for animal cultivation

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Example 1, Preparation of Enterococcus faecalis Liquid for Animal Breeding

[0030] Option I

[0031] Take 35kg of peeled soybean meal, add 350kg of water, soak at room temperature (15-35°C) for 20 hours, grind it with a refiner (into a powder), centrifuge to remove the precipitate, take all the water extract (that is, the supernatant) with a pump The water pump is transferred into a water tank with a capacity of 1000 liters; add 50kg of water, 20kg of glucose and 20kg of sugar beet molasses, and stir at a low speed of 30 to 36rpm; heat the steam to 95°C for 20 minutes; pass the cooling water in the jacket to cool down, At the same time, stir at a low speed of 12-15rpm to keep the temperature of the medium even; when the temperature of the solution in the tank drops below 38°C, insert 8 liters of Enterococcus faecalis seed solution (that is, the volume ratio is 1:50 for inoculation) ), let it stand for 5 minutes, and then divide it into plastic buckets with a volume of...

Embodiment 2

[0050] Example 2, Preparation of Streptococcus thermophilus Liquid for Animal Breeding

[0051] Option I

[0052] Take 35kg of peeled soybean meal, add 350kg of water, soak at room temperature (15-35°C) for 25 hours, grind it with a refiner (into powder), centrifuge to remove the precipitate, and take all the water extract (that is, the supernatant) with a pump The water pump is transferred into a water tank with a volume of 1000 liters; add 50kg of water, 20kg of brown sugar and 20kg of beet molasses, and stir at a low speed of 30 to 36rpm; heat the steam to 100°C for 5 minutes; pass the cooling water in the jacket to cool down, At the same time, stir at a low speed of 12 to 15 rpm to keep the temperature of the medium even; when the temperature of the solution in the tank drops below 38°C, add 200 liters of Streptococcus thermophilus seed solution (that is, the volume ratio is 1:2 Inoculation), let stand for 5 minutes, and then divide into plastic buckets with a volume of 1...

Embodiment 3

[0056] Example 3, Preparation of Enterococcus faecom Liquid for Animal Breeding

[0057] Option I

[0058] Take 35kg of peeled soybean meal, add 350kg of water, soak at room temperature (15-35°C) for 30 hours, grind it with a refiner (into a powder), centrifuge to remove the precipitate, and take all the water extract (that is, the supernatant) with a pump The water pump is transferred into a water tank with a volume of 1000 liters; add 50kg of water, 20kg of glucose and 20kg of beet molasses, stir at a low speed of 30-36rpm; heat to 95-100°C for 10 minutes with steam; pass cooling water through the jacket Cool down, and at the same time stir at a low speed of 12-15rpm to keep the temperature of the culture medium uniform; when the temperature of the solution in the tank drops below 38°C, add 20 liters of Enterococcus faecom seed solution (that is, the volume ratio is 1: 20 inoculations), let it stand for 5 minutes, and then divide it into plastic buckets with a volume of 10 ...

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Abstract

The invention discloses a preparation method of lactic acid bacteria liquid for animal cultivation. The preparation method comprises the following steps of: 1) heating up a fermentation medium to 95-100 DEG C and sterilizing for 5-20 minutes, and cooling to be less than 38 DEG C; 2) inoculating seed solution of homoplastic lactic acid zymocyte into the fermentation medium cooled by the step 1) according to the volume ratio of 1:(2-50) to carry out liquid anaerobic fermentation; and 3) collecting the fermentation liquor fermented by the liquid anaerobic fermentation of the step 2) to obtain the lactic acid bacteria liquid for the animal cultivation. The preparation method of the lactic acid bacteria liquid for the animal cultivation provided by the invention has the following advantages that the preparation method is low in equipment requirement, and low in production cost, the obtained lactic acid bacteria liquid is stable in quality, the lactic acid bacteria liquid can be preferably popularized and used in the cultivation industry, the animal production performance can be improved, and the safety of the animal derived food can be improved.

Description

technical field [0001] The invention relates to a preparation method of lactic acid bacteria liquid for animal breeding. Background technique [0002] Lactic acid bacteria are beneficial bacteria commonly used in the pig and poultry industry. The production cost of liquid lactic acid bacteria is relatively low, and it is a widely used live bacteria preparation, mainly for animals to drink through the drinking water system. Traditional lactic acid bacteria preparations, whether it is solid dry powder or bottled or barreled bacterial liquid, are all produced in a pure culture environment. The production process requires high aseptic conditions, large equipment investment, high energy consumption, and high-temperature sterilization of raw materials. Bacteria, the production cost per ton of liquid preparations is at least more than 5000 yuan, resulting in great difficulties in the popularization and application of lactic acid bacteria preparations. Contents of the invention ...

Claims

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Application Information

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IPC IPC(8): A23K1/00
Inventor 陆文清王春林王园刘绪同
Owner 北京同力兴科农业科技有限公司
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