Preparation method of lactic acid bacteria liquid for animal cultivation
A technology of lactic acid bacteria liquid and animal breeding, applied in animal feed, animal feed, application, etc., can solve the problems of difficult promotion and application of lactic acid bacteria preparations, high requirements for aseptic conditions, and large investment in equipment, so as to improve feed conversion efficiency and increase The effect of average daily gain and low production cost
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[0029] Example 1. Preparation of Enterococcus faecalis liquid for animal breeding
[0030] Scheme I
[0031] Take 35kg of dehulled soybean meal, add 350kg of water, soak at room temperature (15-35℃) for 20 hours, then grind it with a refiner (to powder), centrifuge to remove the precipitate, take all the water extract (ie supernatant) and pump Transfer the water pump to a 1000 liter water tank; add 50 kg of water, 20 kg of glucose and 20 kg of beet molasses, and stir at low speed at 30 to 36 rpm; heat to 95°C for 20 minutes with steam; pass cooling water to the jacket to cool down, At the same time, low-speed stirring at 12-15 rpm keeps the temperature of the medium uniform; when the temperature of the solution in the tank drops below 38°C, 8 liters of Enterococcus faecalis seed solution (that is, the volume ratio is 1:50) ), let it stand for 5 minutes, and then divide it into plastic buckets with a volume of 10 liters. Each bucket is filled with no gaps. The upper loading port i...
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[0050] Example 2. Preparation of Streptococcus thermophilus liquid for animal breeding
[0051] Scheme I
[0052] Take 35kg of dehulled soybean meal, add 350kg of water, soak at room temperature (15-35℃) for 25 hours, grind it with a refiner (to powder), centrifuge to remove precipitation, take all the water extract (ie supernatant) and pump Transfer the water pump to a 1000 liter water tank; add 50 kg of water, 20 kg of brown sugar and 20 kg of beet molasses, stir at low speed at 30 to 36 rpm; steam and heat to 100 ℃ for sterilization for 5 minutes; pass cooling water in the jacket to cool down, At the same time, low-speed stirring at 12-15 rpm keeps the temperature of the culture medium uniform; when the temperature of the solution in the tank drops below 38°C, 200 liters of Streptococcus thermophilus seed solution (that is, the volume ratio is 1:2) Inoculation), let it stand for 5 minutes, and then divide it into plastic buckets with a volume of 10 liters. Each bucket is filled...
Example Embodiment
[0056] Example 3. Preparation of Enterococcus faecom liquid for animal breeding
[0057] Scheme I
[0058] Take 35kg of dehulled soybean meal, add 350kg of water, soak at room temperature (15-35℃) for 30 hours, then grind it with a refiner (to powder), centrifuge to remove the precipitate, take all the water extract (ie supernatant) and pump Transfer the water pump to a 1000 liter water tank; add 50kg water, 20kg glucose and 20kg beet molasses, stir at 30~36rpm at low speed; steam and heat to 95~100℃ for sterilization for 10 minutes; pass cooling water through the jacket Cool down while stirring at a low speed at 12-15 rpm to keep the temperature of the medium uniform; when the temperature of the solution in the tank drops below 38°C, insert 20 liters of Enterococcus faecom seed solution (that is, the volume ratio is 1: 20 inoculation), let it stand for 5 minutes, and then divide it into plastic buckets with a volume of 10 liters. Each bucket is filled with no gaps. Close the upp...
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