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Application of micro RNA (Ribonucleic Acid) gene miR-1291 on predication of prognosis of breast cancer

A breast cancer and gene technology, applied in the field of biomarkers for personalized treatment, can solve problems such as different curative effects and toxic and side effects, prognosis, different curative effect characteristics, and failure to meet clinical requirements, etc., to achieve the effect of improving prognosis

Inactive Publication Date: 2013-03-13
苏州市思玛特电力科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, due to the heterogeneity of malignant tumors, the above-mentioned typing and classification systems still cannot meet the clinical requirements. Patients of the same subtype (or risk group) have different prognosis and curative effect characteristics.
The latest research shows that malignant tumor, as a polygenic genetic disease, is the result of multiple factors and multiple signal transduction pathways, as well as the result of the interaction between the tumor and the immune system in the body, and even tumor cells in different regions of the same tumor tissue Its genomic characteristics may be different, and this high degree of heterogeneity (heterogeneity) leads to different efficacy and side effects of the same drug in different patients

Method used

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  • Application of micro RNA (Ribonucleic Acid) gene miR-1291 on predication of prognosis of breast cancer
  • Application of micro RNA (Ribonucleic Acid) gene miR-1291 on predication of prognosis of breast cancer

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Experimental program
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Embodiment 1

[0029] Embodiment 1, breast cancer prognosis prediction

[0030] Using mirVana RNA Isolation Kit (Applied Biosystem p / n AM1556), according to the operation steps provided in the kit, total RNA was extracted from surgical resection samples of breast cancer patients, and stored in aliquots.

[0031] RNA quality detection. UV absorbance assay was used to measure the absorbance at wavelengths of 260nm and 280nm to determine the concentration of RNA samples, and combined with agarose gel electrophoresis to detect the quality of RNA.

[0032] The chip hybridization experiment was carried out in strict accordance with the operation manual of Agilent Human 8*60K miRNA chip. The basic process includes dephosphorylation, sample denaturation, ligation labeling, chip hybridization, chip washing, chip scanning and other steps.

[0033] The chip results were scanned with an Agilent scanner, and the Feature Extraction software read the data Scanresolution 5 μm, PMT 100%, and processed and a...

Embodiment 2

[0035] Example 2, real-time quantitative PCR detection of mir-1291 gene expression

[0036] The extraction and quality detection of RNA were as described in Example 1. Using Ribo's mir-1291Bulge-Loop TM miRNA qPCR detection kit detection. Wherein, the cDNA chain is synthesized by reverse transcription using the stem convex loop primer, and the real-time quantitative PCR amplification is performed using the synthesized cDNA as a template. Each sample was tested three times, and the content of U6snRNA or 5S rRNA in each sample was detected as an internal reference to correct the loading error, analyze the relative expression level, and compare the expression differences of different patients. The results showed that in a group of 52 breast cancer patients, the difference in the expression of mir-1291 was 2.8 times between breast cancer patients without metastases within 5 years and those with metastases within 5 years (p value < 0.0001).

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Abstract

The invention discloses an application of micro RNA (Ribonucleic Acid) gene miR-1291 in preparation of a product for predicating prognosis of breast cancer. The invention also discloses an application of the miR-1291 in preparation of screening of medicines for treating tumor. The RNA gene miR-1291 and the miR-1291 gene related to transfer of breast cancer can be used as markers for early diagnosing the transfer of the tumor in order to provide assistance to doctors to develop individual-based treatment schemes, and also can be used as pharmacotherapy targets for controlling the transfer of the tumor.

Description

technical field [0001] The field of the present invention belongs to the field of gene diagnosis and individualized treatment of tumors, specifically, the biological markers for predicting the prognosis of breast cancer patients by using the gene expression characteristics of patients to adopt individualized treatment. Background technique [0002] Breast cancer is the most common female tumor. Due to the heterogeneity of breast cancer, there is currently no single clinical molecular marker (such as Bcl-2, p53, MDR-1, etc.) that can accurately and effectively predict the efficacy of drug chemotherapy (including neoadjuvant therapy). Based on the gene expression profile analysis at the whole genome level, according to a widely recognized molecular pathological classification system of breast cancer, breast cancer can be divided into the following subtypes: (1) basal-like breast cancer, mainly Characterized by estrogen receptor (ER) negative and HER2 gene negative; (2) lumina...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 杭兴宜熊江辉于国强汤奕王晨杨正茂魏维
Owner 苏州市思玛特电力科技有限公司