Biological aerosol water-content determination based on near infrared spectroscopy and classification method thereof

A bioaerosol and near-infrared spectroscopy technology, which is applied in the field of bioaerosol water content determination and classification, can solve the problems of poor real-time performance, inability to distinguish biological particles, and inability to determine whether the threat of biological warfare agents exists, and achieve effective identification. , Overcome the effect of poor real-time performance and difficult implementation

Active Publication Date: 2013-03-13
ELECTRONICS ENG COLLEGE PLA
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Problems solved by technology

However, whether it is fluorescence method, microscope method or scattering method, they all have their own shortcomings.
For example, although the fluorescence method can effectively detect biological aerosols, it cannot judge whether the threat of biological warfare agents exists; the micro

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  • Biological aerosol water-content determination based on near infrared spectroscopy and classification method thereof
  • Biological aerosol water-content determination based on near infrared spectroscopy and classification method thereof
  • Biological aerosol water-content determination based on near infrared spectroscopy and classification method thereof

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[0014] A method for measuring and classifying the water content of biological aerosols based on near-infrared spectroscopy, the method includes the steps in the following sequence: the first step is to collect and calculate the biogas to be detected by using the open FTIR atmospheric transmission spectrometry system The near-infrared transmittance spectrum t(λ) of the sol; the average particle diameter D of the biological particles in the biological aerosol to be detected is measured by using an optical particle counter 32 ; The second step, according to the near-infrared transmittance spectrum t(λ) of bioaerosol and the average particle diameter D of biological particles in bioaerosol 32 , inverse calculation of the real part n of the infrared complex refractive index of biological particles in the bioaerosol; the third step, according to the real part n of the infrared complex refractive index of the biological particles in the bioaerosol, back calculation of the content of b...

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Abstract

The invention relates to a biological aerosol water-content determination based on a near infrared spectroscopy and a classification method thereof. The method comprises using an open FTIR atmospheric transmission spectrum determination system for collecting and calculating near-infrared transmittance spectra of the biological aerosol to be detected; using an optical particle counter for determining an average particle size of biological particles in the biological aerosol to be detected; inversely calculating a real part of an infrared complex refractive index of the biological particles in the biological aerosol according to the near-infrared transmittance spectra of the biological aerosol and the average particle size of the biological particles in the biological aerosol; inversely calculating the water content of the biological particles in the biological aerosol according to the infrared complex refractive index of the biological particles in the biological aerosol; and classifying the to-be-detected biological aerosol according to water-content differences of different types of biological particles. An online identification of the biological aerosol can be realized by employing concise experiment steps, thereby overcoming problems of difficult implement and poor real time existing in present identification methods of the biological aerosol.

Description

technical field [0001] The invention relates to the field of optical detection, in particular to a method for measuring and classifying the water content of biological aerosols based on near-infrared spectroscopy. Background technique [0002] As an important part of atmospheric aerosol, bioaerosol has a huge impact on human production and life. With the increasing development of biotechnology in human society, bioaerosols in the atmosphere are more and more closely related to human activities. The development of effective bioaerosol identification methods is of great significance for accurately evaluating the ambient air quality environment and actively protecting against biological warfare agents. [0003] At present, the detection and identification methods of bioaerosol mainly include fluorescence method, microscope method and scattering method. Ultraviolet luminescent groups such as Tryptophan, Nicotinamide adenine nucleotides phosphate and Riboflavin in bioaerosols wi...

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Application Information

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IPC IPC(8): G01N21/25G01N21/41
Inventor 胡以华雷武虎顾有林孙杜娟赵楠翔石亮王勇郑之明
Owner ELECTRONICS ENG COLLEGE PLA
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