Biological aerosol water-content determination based on near infrared spectroscopy and classification method thereof

Abstract

The invention relates to a biological aerosol water-content determination based on a near infrared spectroscopy and a classification method thereof. The method comprises using an open FTIR atmospheric transmission spectrum determination system for collecting and calculating near-infrared transmittance spectra of the biological aerosol to be detected; using an optical particle counter for determining an average particle size of biological particles in the biological aerosol to be detected; inversely calculating a real part of an infrared complex refractive index of the biological particles in the biological aerosol according to the near-infrared transmittance spectra of the biological aerosol and the average particle size of the biological particles in the biological aerosol; inversely calculating the water content of the biological particles in the biological aerosol according to the infrared complex refractive index of the biological particles in the biological aerosol; and classifying the to-be-detected biological aerosol according to water-content differences of different types of biological particles. An online identification of the biological aerosol can be realized by employing concise experiment steps, thereby overcoming problems of difficult implement and poor real time existing in present identification methods of the biological aerosol.

Description

technical field [0001] The invention relates to the field of optical detection, in particular to a method for measuring and classifying the water content of biological aerosols based on near-infrared spectroscopy. Background technique [0002] As an important part of atmospheric aerosol, bioaerosol has a huge impact on human production and life. With the increasing development of biotechnology in human society, bioaerosols in the atmosphere are more and more closely related to human activities. The development of effective bioaerosol identification methods is of great significance for accurately evaluating the ambient air quality environment and actively protecting against biological warfare agents. [0003] At present, the detection and identification methods of bioaerosol mainly include fluorescence method, microscope method and scattering method. Ultraviolet luminescent groups such as Tryptophan, Nicotinamide adenine nucleotides phosphate and Riboflavin in bioaerosols wi...

AI Technical Summary

Problems solved by technology

However, whether it is fluorescence method, microscope method or scattering method, they all have their own shortcomings.

For example, although the fluorescence method can effectively detect biological aerosols, it cannot judge whether the threat of biological warfare agents exists; the microscope method can effectively identify the types of biological aerosols, but the real-time performance is poor and the experimental process is cumbersome; the scattering method cannot distinguish the same shape and size but the type Biological particles with different and similar composition

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