Modification method and application of cellulosa-imitating film structure of polylactic acid nano-particle surface
A technology imitating the outer membrane of cells and nanoparticles, which is applied in the field of preparation of polylactic acid drug-loaded nanoparticles, can solve problems such as shedding and reducing the modification effect, and achieve the effect of improving the controlled release of drugs and improving the long-term circulation performance in the body
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Embodiment 1
[0043] 5 mL of 6 mg / mL polylactic acid acetone solution and 4 mL of 2 mg / mL PMBT ethanol solution were simultaneously dropped into 40 ml of water under stirring, and the dropwise addition was completed in 20 minutes. Form an opalescent polylactic acid nanodispersion, adjust the pH to 8.5 with 4% sodium hydroxide aqueous solution, and continue to stir to promote the crosslinking reaction between the PMBT polymer chains in the polylactic acid nanoparticle stable coating. Transfer the dispersion to two 50 mL centrifuge tubes, and centrifuge at 12,000 rpm for 20 minutes at 0-4°C. The supernatant was discarded, the precipitate was redispersed with distilled water, the precipitate was centrifuged and washed three times under the same conditions, and finally redispersed with 10 mL of distilled water, and placed in the refrigerator for later use. The average particle size was measured by dynamic light scattering to be 180 nm.
Embodiment 2
[0045] Weigh 0.0224 g of doxorubicin hydrochloride into a 150 mL beaker, add 40 mL of an aqueous solution containing 30% volume of acetone, adjust the pH to 7.54 with 0.4% sodium hydroxide solution, stir for 3 minutes, and mix 4 mL of 15 mg / mL The polylactic acid acetone solution and 4 mL 3.0 mg / mL PMBT methanol solution were dropped into the doxorubicin solution at the same time, and the dropwise addition was completed in 20 minutes. The pH was adjusted to 8.5 with 4% sodium hydroxide aqueous solution, and the stirring was continued for three days. After filtering through a G 3 sand core funnel, pour it into a 50 mL centrifuge tube, and centrifuge at 12000 rpm at 0-4 °C for 20 minutes. Discard the supernatant, redisperse the precipitate with distilled water, centrifuge and wash the precipitate three times under the same conditions, and finally redisperse with 15 mL of distilled water, and put it in the refrigerator for later use. like figure 1 As shown, the average particle ...
Embodiment 3
[0047] Weigh 0.0200 g of doxorubicin hydrochloride into a 150 mL beaker, add 40 mL of an aqueous solution containing 30% volume of acetone, adjust the pH to 7.54 with 0.4% sodium hydroxide solution, stir for 3 minutes, and add 4 mL of 15 mg / mL The polylactic acid acetone solution and 4 mL 3.5 mg / mL PMB methanol solution were dropped into the doxorubicin solution at the same time, and the dropwise addition was completed in 20 minutes, and the stirring was continued for three days. After filtering through a G 3 sand core funnel, pour it into a 50 mL centrifuge tube, and centrifuge at 12000 rpm at 0-4 °C for 20 minutes. The supernatant was discarded, the precipitate was redispersed with distilled water, the precipitate was centrifuged and washed three times under the same conditions, and finally redispersed with 15 mL of distilled water, and stored in the refrigerator for later use. The average particle size was measured by dynamic light scattering to be 224 nm.
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