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Culture medium special for preventing brown stain of butterfly orchids

A medium and a technology for preventing browning, applied in the field of plant tissue culture, can solve problems such as browning, and achieve the effects of preventing browning, reducing production and expansion, and enhancing activity

Inactive Publication Date: 2013-03-27
SUZHOU HEMEI BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the disadvantage of this method is that it can only passively solve the phenolic compounds diffused into the culture substrate, and there will still be a high browning phenomenon in the actual production process

Method used

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  • Culture medium special for preventing brown stain of butterfly orchids

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Experimental program
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Embodiment

[0017] Take the flower stalks with dormant buds that have passed the full flowering stage as explant materials, cut off the stems with buds, about 2-3cm long, clean them with tap water, soak them in saturated bleach supernatant for 15 minutes, and keep stirring when soaking , the soaked stems were rinsed with running water, placed on the ultra-clean workbench, first sterilized with 75% alcohol for 30 s, washed once with sterile water, and then soaked in 0.1% mercury chloride for 10 min, after Rinse several times with sterile water, inoculate the budding stems on the designed induction medium with different hormone combinations, and repeat 3 times.

[0018] The induction medium is 1 / 2 MS + BA 2.5 mg / L + NAA 0.2 mg / L as the basal medium, and ascorbic acid (VC) at a concentration of 5-15 mg / L and brassinosteroids at a concentration of 0.001-0.5 mg / L are added lactone (BR), where the pH is adjusted to 5.4-5.6.

[0019] Phalaenopsis anti-browning special medium with different conc...

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PUM

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Abstract

The invention discloses a culture medium special for preventing brown stain of butterfly orchids, which is prepared by taking a 1 / 2 MS culture medium containing 2.5 mg / L of benzyl amino adenine (BA) and 0.2 mg / L of naphthylacetic acid (NAA) as a basic culture medium and adding vitamin C (VC) with the concentration of 5-15 mg / L and brassinolide (BR) with the concentration of 0.001-0.5 mg / L. The culture medium disclosed by the invention is capable of greatly reducing the brown stain rate in the butterfly orchid tissue culture process.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a culture medium for preventing browning in the tissue culture process of Phalaenopsis. technical background [0002] Phalaenopsis [phalaenopsis] is a plant of the genus Phalaenopsis in the family Orchidaceae. It is a tropical aerial orchid, commonly known as "ocean orchid". Phalaenopsis is butterfly-like in shape, beautiful in shape, rich in color and long in flowering period. It is known as the "Queen of Orchids" among tropical orchids. Phalaenopsis is rich in species and widely distributed. It starts from the Philippines and New Guinea in the east, reaches northern Australia in the south, and West Sumatra in the south. There are original species (wild Phalaenopsis) in Taiwan, Yunnan, and western Sichuan in the north. There are about 50 kinds of them. There are 7 species in my country, all of which are epiphytic orchids. The large-scale commercial cultivation of P...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 毕德
Owner SUZHOU HEMEI BIOTECH
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