Competitive inhibition enzyme-linked immunosorbent assay (ELISA) method for grass carp tumor necrosis factors alpha
A tumor necrosis factor and enzyme-linked immunosorbent assay technology, which is used in measurement devices, biological tests, and material inspection products, can solve the problem of not specifically detecting the alpha concentration of grass carp tumor necrosis factor, and achieve a fast and efficient detection method with low cost. Effect
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[0016] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.
[0017] An enzyme-linked immunoassay method for competitive inhibition of grass carp tumor necrosis factor alpha, using recombinant grass carp tumor necrosis factor alpha protein as an immunogen to immunize healthy New Zealand white rabbits to obtain a polyclonal antibody (grass carp tumor necrosis factor alpha polyclonal antibody), and then use The polyclonal antibody was labeled with horseradish peroxidase (HRP) to obtain the HRP-labeled grass carp tumor necrosis factor alpha polyclonal antibody (hereinafter referred to as enzyme-labeled antibody). Finally, the recombinant grass carp tumor necrosis factor alpha protein was used again as the coating antigen and enzyme-labeled antibody to establish a competitive inhibition enzyme-linked immunosorbent immunoassay (ELISA) method for grass carp tumor necrosis factor alpha (TNFα).
[0018] The above...
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