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Material sterilization method integrating continuous sterilization and filled can sterilization in lysine fermentation process

A technology of lysine fermentation and sterilization method, which is applied in the field of medium sterilization technology, can solve the problems of large medium damage, high energy consumption, heat exchanger fouling, etc., and achieve the goal of overcoming the destruction of medium and energy High consumption, the effect of solving scaling

Active Publication Date: 2014-05-28
LINGHUA GROUP INCORPORATED COMPANY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The advantage of continuous sterilization is that the consumption of steam and cooling water can be saved through the heat exchanger. At the same time, since heating and cooling are completed in a very short time, it can reduce the destruction of the medium and improve the quality of the medium. Therefore, for large-volume fermentation Tanks generally use the continuous elimination method; however, there is also a problem that is difficult to solve in the continuous elimination process, that is, some Pellanite components are easy to cause fouling of the heat exchanger, and the workload of cleaning the heat exchanger is huge, which affects the batch of tanks. At the same time, it affects the heat exchange effect and even leads to incomplete sterilization.
The advantage of solid tank sterilization is that the equipment is simple, there is no fouling problem in the heat exchanger, and the sterilization effect is more reliable than continuous sterilization. The damage is large, and at the same time, because there is no heat exchange device, the energy consumption is high

Method used

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  • Material sterilization method integrating continuous sterilization and filled can sterilization in lysine fermentation process
  • Material sterilization method integrating continuous sterilization and filled can sterilization in lysine fermentation process

Examples

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Embodiment 1

[0022] Example 1 The material sterilization method of combining Lianshixiao in lysine fermentation

[0023] (1) Fermentation medium composition: corn steep liquor, glucose, phosphoric acid, magnesium sulfate, potassium chloride and soybean meal hydrolyzate (specifically, the weight ratio of each component of the fermentation medium to the total fermentation material is: corn steep liquor 2%, glucose 10-12%, potassium dihydrogen phosphate 0.3-0.4%, magnesium sulfate 0.3-0.4%, soybean meal hydrolyzate 2%, ammonium sulfate 3%.

[0024] (2) Real consumption: mix corn steep liquor, dipotassium hydrogen phosphate, soybean meal hydrolyzate, and ammonium sulfate in the fermentation medium, add ingredient water, control the volume to 15% of the total fermentation material volume, and put it into the fermentation tank. Raise the temperature to 121°C with steam from the bottom and keep it for 50 minutes;

[0025] (3) Continuous elimination: mix glucose and magnesium sulfate, add ingredi...

Embodiment 2

[0027] Example 2 The material sterilization method of combining Lianshi Xiaoxiao in lysine fermentation

[0028] 1. Composition of fermentation medium:

[0029] Corn steep liquor 2%, glucose 10-12%, potassium dihydrogen phosphate 0.3-0.4%, magnesium sulfate 0.3-0.4%, soybean meal hydrolyzate 2%, ammonium sulfate 3%.

[0030] 2. Mix corn steep liquor, potassium dihydrogen phosphate, soybean meal hydrolyzate, and ammonium sulfate, and put them directly into the tank to be sterilized, and add ingredient water to make the volume reach 15% of the total volume.

[0031] 3. The fermenter (including the mixture of 2 steps) is heated from the bottom by steam, so that the temperature reaches 120°C--123°C, and keeps for 40-60 minutes.

[0032] 4. Mix other components except the medium material in step 2 together, and add batching water to make up the volume, raise the temperature to 125°C-128°C through the continuous elimination device, maintain the temperature in the tank for 8-10 minu...

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Abstract

The invention discloses a material sterilization method integrating continuous sterilization and filled can sterilization in a lysine fermentation process. The material sterilization method is characterized in that the material sterilization method comprises the steps that components of a fermentation culture medium are ascertained; the filled can sterilization, the continuous sterilization are conducted on the culture medium according to actual conditions; and then the inoculation fermentation is conducted. With the adoption of the material sterilization method, a continuous-sterilization heat exchanger is not required to be cleaned frequently; the fermentation does not cause pollution due to the fact that the continuous-sterilization heat exchanger is not cleaned frequently; the disinfection effect is better; and the steam consumption and the cooling water consumption are reduced remarkably.

Description

technical field [0001] The invention belongs to the technical field of lysine fermentation production, and in particular relates to the technology of culture medium sterilization. Background technique [0002] The lysine fermentation process requires high-temperature sterilization of the medium. There are generally two ways to sterilize: one is solid tank sterilization, that is, the prepared medium is put into the fermenter (or seed tank) in advance, The temperature is raised in the tank, and after reaching the required temperature, it is maintained for a certain period of time, and the temperature is lowered to the temperature required for fermentation to inoculate and ferment. The second is continuous sterilization, that is, the fermenter (or seed tank) is pre-empty and sterilized, and then the prepared medium is heated through a continuous sterilizer, the temperature of the tank is maintained for a certain period of time, and the temperature of the heat exchanger is coole...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L2/07
Inventor 杨玉岭岳希金李高卫满德恩郭脉海程美科
Owner LINGHUA GROUP INCORPORATED COMPANY
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