Contaminated soil phenanthrene and application thereof in contaminated soil restoration

A technology of polluting soil and degrading bacteria, applied in the restoration of polluted soil, bacteria, and microbial-based methods, etc., can solve problems such as the impact of competition, soil ecological risks, etc.

Inactive Publication Date: 2013-04-03
XINJIANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Microbial remediation often requires the introduction of exogenous microorganisms into the soil. The degradation effect of these introduced obligate degrading bacteria or genetically engineered bacteria is easily affected by the competition of indigenous microorganisms, and the introduction of exogenous bacteria also has potential soil ecological problems. risk

Method used

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  • Contaminated soil phenanthrene and application thereof in contaminated soil restoration
  • Contaminated soil phenanthrene and application thereof in contaminated soil restoration
  • Contaminated soil phenanthrene and application thereof in contaminated soil restoration

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Pseudomonas geniculata CGMCC No. Isolation, screening and identification of 6763

[0026]Samples were taken from oil-contaminated soil in Karamay Oilfield, 5g soil samples were placed in 50mL phenanthrene inorganic salt medium, 30°C, 150r / min shaking culture, when OD600=0.6-0.8 (bacteria in logarithmic growth phase), transfer Take 1 mL of the culture solution and add it to 60 mL of water-silicone oil dual-phase system culture medium with a p (phenanthrene) of 100 mg / L. Water-silicone oil forms a dual-phase system at a volume ratio of 4:1. Shake at 30°C and 150 r / min in the dark nourish. When the OD600 value of the culture solution is 0.6-0.8, pipette 1 mL of the culture solution and add it to the newly prepared medium containing phenanthrene inorganic salts. The above steps were repeated for a total of 8 generations of enrichment culture.

[0027] Take 1 mL of the enriched culture solution, make a phenanthrene plate, incubate in a 30°C incubator for 7 days...

Embodiment 2

[0038] Example 2: Pseudomonas geniculata CGMCC No. 6763 growth factor

[0039] See the following method, but it should be based on the Pseudomonasgeniculata CGMCC No. provided by the present invention. 6763 is characterized by its specific growth factor.

[0040] The strain y-8 was cultured, and the results are shown in Table 2, and the strain y-8 is the most suitable growth factor.

[0041] Table 2: Effects of temperature, pH, salt, antibiotics on the growth of strain y-8

[0042] temperature(℃) 4 10 15 20 25 30 growing situation + + + ++ +++ ++++ temperature(℃) 35 40 45 50 55 60 growing situation ++ + + - - -

[0043] pH 1 2 3 4 5 6 growing situation - - - - + + pH 7 8 9 10 growing situation ++ + + + NaCl concentration 0.5％ 1％ 2％ 3％ 4％ 5％ growing situation + ++ ++ ++ + - NaCl concentration 6％ 7％ 8％ 9％ 10％ growing situation ...

Embodiment 3

[0044] Example 3: Determination of Pseudomonas geniculata CGMCC No.6763 on the degradation performance of phenanthrene

[0045] Inoculate Pseudomonas geniculata (Pseudomonas geniculata) CGMCC No.6763 into inorganic salt medium containing 100 mg / L phenanthrene, culture at 150 rpm / min, shaker at 30°C in the dark, at the 0th, 8th, 16th, Samples were taken at 24, 32, 40, 48, 56, 64, 72, and 80 hours respectively to determine the bacterial density and phenanthrene residue rate in the culture solution. For the experimental results, see the attached Figure 4 . attached by Figure 4 It can be seen from the degradation curve of phenanthrene that at the initial concentration of 100mg / L, the degradation of phenanthrene is slow in the initial stage of 0-16h, and the degradation of phenanthrene is very fast in the next 16-64h. By 72h, the degradation amount of phenanthrene has reached 91.22mg. Also, see attached Figure 4 The cell growth curve shows that the cell growth is a little sl...

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Abstract

The invention discloses a contaminated soil phenanthrene and an application thereof in contained soil restoration. A bacterial strain with a contaminated soil restoration function is obtained by screening the strain in kelamayi oilfield oil-contaminated soil sample; the bacteria y-8 is determined as pseudomonasgeniculata CGMCC (China General Microbiological Culture Collection Center) No.6763 by further screening, domesticating and breeding, and carrying out morphological characteristic, 16SrRNA sequence determination and phylogenetic analysis to the obtained bacterial strain; according to the measured degradation property of the phenanthrene and the intensive study of the action of the phenanthrene in contaminated soil restoration, the contaminated soil phenanthrene has an outstanding technical effect in the contaminated soil restoration.

Description

technical field [0001] The invention relates to the technical field of application of soil remediation microorganisms, in particular to the technical field of a phenanthrene-contaminated soil-degrading bacterium and its application in the remediation of phenanthrene-contaminated soil. Background technique [0002] Polycyclic aromatic hydrocarbons (PAHs) refer to a class of compounds in which two or more aromatic rings are fused together, and are ubiquitous in the environment. It has strong carcinogenicity, mutagenicity and teratogenicity, and has a strong inhibitory effect on microbial growth, and polycyclic aromatic hydrocarbons are more toxic after being irradiated by ultraviolet light. As early as the early 1980s, the U.S. Environmental Protection Agency identified 16 unbranched polycyclic aromatic hydrocarbons as priority pollutants in the environment. my country also included polycyclic aromatic hydrocarbons in the blacklist of pollutants for environmental priority moni...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/38
Inventor 晁群芳陈志丹杨槟银李磊王旭辉尹丹丹方新湘
Owner XINJIANG UNIVERSITY
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