Culture method for same-embryo proliferation of new castle disease and infectious bronchitis viruses
A technology for bronchitis and Newcastle disease, applied in the direction of virus/bacteriophage, biochemical equipment and methods, microorganisms, etc., can solve the problems of time-consuming and laborious, high production cost, waste of materials, etc., achieve quick operation, simplify production steps, and reduce production cost effect
Active Publication Date: 2014-07-23
山东滨州沃华生物工程有限公司
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Problems solved by technology
At present, the usual method of preparing chicken Newcastle disease and infectious bronchitis vaccines is to prepare the two viruses separately, and then mix them in a certain proportion to make vaccines, which is time-consuming and laborious, and the production cost is also high.
Therefore, in view of the current situation that the existing chicken Newcastle disease and infectious bronchitis are cultured separately, time-consuming, laborious, and material-consuming, we urgently need to improve the culture methods of Newcastle disease and infectious bronchitis to achieve the purpose of cultivating two antigens at one time
Method used
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Embodiment
[0016] Embodiment: The cultivation method of homeomorphic chicken Newcastle disease and infectious bronchitis virus
[0017] In this example, the LaSota strain of the Newcastle disease virus and the M41 strain of the infectious bronchitis virus were purchased from the National Institute for the Control of Pharmaceutical and Biological Products.
[0018] 1. Culture method and steps
[0019] (1) Dilute the Newcastle disease virus seed LaSota strain to 10 5 EID 50 、10 6 EID 50 、10 7 EID 50 Three groups, the infectious bronchitis strain M41 was diluted into 10 3 EID 50 、10 4 EID 50 、10 5 EID 50 , after dilution, mix the two virus solutions in a ratio of 1:1;
[0020] (2) Inoculate the mixed virus seeds into the allantois of 9, 10, and 11-day-old susceptible chicken embryos, inoculate 0.15ml of the allantoic cavity in each embryo, and seal the inoculation holes and stomata with wax after inoculation;
[0021] (3) Put the inoculated chicken embryos into the incubator, a...
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The invention discloses a method for same-embryo proliferation cultivation of new castle disease and infectious bronchitis viruses. The culture method comprises the followings steps: inoculating the new castle disease and infectious bronchitis viruses at the same embryo; symmetrically adjusting the hatching temperature, inoculation age in days, embryo collecting time, and the concentration and proportion of the new castle disease and infectious bronchitis viruses to reach the optimal combination; and collecting allantoic fluid antigen from the chick embryo through the processes such as hatching, cooling, disinfecting and the like. The culture method provided by the invention increases the yield of the antigen, simplifies the production steps, saves the time and materials, reduces the production cost, is quicker to operate, has better economical benefits and social benefits, and is convenient to carry out, popularize and apply.
Description
technical field [0001] The invention relates to the technical field of cultivation of veterinary biological products, in particular to a cultivation method for homeomorphic proliferation of chicken Newcastle disease and infectious bronchitis virus. Background technique [0002] Newcastle disease (Newcastle disease) is an acute, highly contagious infectious disease of chickens caused by Newcastle disease virus (NDV). as the main feature. The disease has an acute onset, high infection rate and mortality rate, sometimes even as high as 100%, causing immeasurable economic losses to the poultry industry, especially the chicken industry. It is a class A infectious disease. [0003] NDV has hemagglutination (HA), and the hemagglutinin on the fibrils on the surface of the NDV capsule can bind to receptors on the surface of red blood cells, causing red blood cell agglutination. NDV can agglutinate red blood cells of all amphibians, reptiles, birds, mice, and guinea pigs, but the a...
Claims
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IPC IPC(8): C12N7/00C12N7/02
Inventor 郭显坡陈申秒牛成明杨灵芝董新荣
Owner 山东滨州沃华生物工程有限公司