Enzyme-linked immunoassay kit and method for furaltadone metabolite detection

A technology of furaltadone and its metabolites, applied in the field of enzyme-linked immunoassay, can solve cancer and other problems
CN103018449AActive Publication Date: 2013-04-03BEIJING KWINBON BIOTECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
BEIJING KWINBON BIOTECH
Publication Date
2013-04-03

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Abstract

The present invention provides an enzyme-linked immunoassay kit and a method for furaltadone metabolite detection. The enzyme-linked immunoassay kit comprises an enzyme label plate coated with coating antigen, specific furaltadone metabolite antibody, an enzyme marker, furaltadone metabolite standard solutions, a substrate coloration solution, a termination solution, a concentration washing solution, a concentration reconstituted solution, and 2-nitrobenzaldehyde. The present invention further provides a furaltadone metabolite enzyme-linked immunoassay kit detection method, which mainly comprises: carrying out a sample pretreatment, adopting the kit to detect, and finally analyzing a detection result. With the detection kit, furaltadone metabolite drugs in animal tissues, aquatic products and honey can be rapidly detected, characteristics of simple and rapid operation, high accuracy, high sensitivity, low cost and the like are provided, and the kit and the method are applicable for screening and on-site monitoring of a large number of samples.
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Description

technical field

[0001] The invention relates to an ELISA detection technology, in particular to an ELISA kit and a detection method for detecting furaltadone metabolites in animal tissues (muscle, liver), aquatic products and honey. Background technique

[0002] Nitrofuran drugs have been widely used as growth-promoting additives for poultry, aquatic products and pigs because of their excellent antibacterial and pharmacokinetic properties. However, in the course of long-term experimental research, it was found that both nitrofuran drugs and metabolites can cause cancer and gene mutations in experimental animals, which is why these drugs are prohibited from being used in treatment and feed.

[0003] Since the prototype drug of nitrofuran is rapidly metabolized in the body, it cannot be detected, but its metabolites are quite stable due to the combination with proteins, so when analyzing the residues of such drugs, it is often necessary to analyze the metabolized products, and...

Claims

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