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Method for preparing autologous skin cell agent for alleviating or treating skin defects

A cell therapy and skin defect technology, applied in skin diseases, medical raw materials derived from mammals, medical preparations containing active ingredients, etc., can solve problems such as inapplicable skin and long time required for cell culture

Inactive Publication Date: 2013-04-10
松家咨询集团
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, conventional cell therapy agents have the problem that cell culture takes a long time, and they are not suitable for severe skin defects such as burns and patients requiring emergency treatment.

Method used

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  • Method for preparing autologous skin cell agent for alleviating or treating skin defects
  • Method for preparing autologous skin cell agent for alleviating or treating skin defects
  • Method for preparing autologous skin cell agent for alleviating or treating skin defects

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0034] figure 1 It is a flowchart of the preparation method of the cell therapy agent of the present invention.

[0035] Such as figure 1 As shown, the preparation method of the cell therapy agent of the present invention includes: the step of obtaining normal skin tissue from a donor site derived from autologous skin tissue (step S11); the step of crushing the above-mentioned obtained normal skin tissue into cell units (step S12); and a step of obtaining a skin cell group comprising at least one of keratinocytes, melanocytes and fibroblasts from the pulverized tissue fragments (step S13).

[0036] The preparation method of the cell therapy agent of the present invention is used to prepare the cell therapy agent for alleviating or treating skin defects in mammals. Preferably, it is used to prepare a cell therapy agent for alleviating or treating human skin defects.

[0037] In the step of obtaining the above normal skin tissue, the normal skin tissue can be obtained from th...

experiment example 1

[0053] Experimental example 1. Cell test contained in the cell therapy agent prepared according to an embodiment of the present invention and an example of application to the affected part

[0054] For 47-year-old women and 36-year-old women, 20 normal skin tissues were cut out with a size of 4×5mm by using a skin harvester, washed with normal saline and then sterilized. Add 2 cc of sterilized water to the above-mentioned sterilized tissue, and grind it for about 30 seconds using a tissue grinder.

[0055] After the above pulverization was completed, a cell strainer was used to obtain skin cell groups including at least one of keratinocytes, melanocytes, and fibroblasts from the tissue fragments, thereby preparing a 47-year-old female autologous A cell therapy agent for skin cells and a cell therapy agent using autologous skin cells of a 36-year-old woman.

[0056] 1-1. Cell concentration measurement

[0057] The cell concentrations of the cell therapy agent using autologous...

Embodiment 2

[0084] Example 2. Application example 2 of the cell therapy agent prepared according to an embodiment of the present invention

[0085] For a 49-year-old male, 20 normal skin tissues with a size of 3×4mm were cut out using a skin harvester, washed with normal saline and then sterilized. Add 2 cc of sterilized water to the above-mentioned sterilized tissue, and grind it for about 30 seconds using a tissue grinder.

[0086] After the above pulverization was completed, a cell strainer was used to obtain skin cell groups containing at least one of keratinocytes, melanocytes, and fibroblasts from the tissue fragments, thereby preparing a 49-year-old male autologous Cell therapy for skin cells.

[0087] First, a biological tissue adhesive (fibrin preparation) was sprayed on the burnt part (cheek and neck) of the above-mentioned 49-year-old man, a cell therapy agent containing the above-mentioned autologous skin cells was sprayed, and a bandage was applied. After using the above-me...

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Abstract

The present invention relates to a method for preparing an autologous skin cell agent for alleviating or treating skin defects. The method for preparing a therapeutic agent for alleviating or treating a skin defect using autologous skin cells according to the present invention, comprises the following steps: obtaining normal skin tissue from the patient's body; mincing the obtained tissue into cell units; and obtaining from the minced tissue matters, a skin cell line including keratinocytes, melanocytes and fibroblasts. The present invention excludes an enzyme-treating and / or an incubation step unlike conventional methods for preparing a therapeutic agent, therefore enabling a considerably easier and faster preparation and quicker application to an affected area.

Description

technical field [0001] The present invention relates to a method for preparing an autologous skin cell therapy agent for relieving or treating skin defects, and more particularly relates to a method for preparing an autologous skin cell therapy agent that is different from the existing cell therapy agent because it can eliminate enzymes or culturing steps in a short period of time. A method of preparing a cellular therapeutic. Background technique [0002] In the case of burns, wounds, depigmentation and other defects in skin tissue, if proper treatment is not carried out or wrong emergency measures are taken, it will leave scars on the skin or prolong the treatment time, and may also be accompanied by other skin diseases. [0003] Generally, in the case of burns or wounds, the skin has been treated by wrapping the skin with a bandage soaked in an ointment mainly composed of petroleum jelly, and additional antibiotics such as penicillin and sulfonamide are administered to pr...

Claims

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Application Information

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IPC IPC(8): A61K35/12A61P17/02A61P17/00A61K35/36
CPCA61K35/12A61K35/36A61P17/00A61P17/02
Inventor 李贞淑郑孝先李正姬闰天在孙泰植金庆植
Owner 松家咨询集团
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