Method for directly inducing cluster buds of milletia speciosa champ seeds and quickly breeding seedlings

A technology of cattle vigorously and seeds, applied in the field of agricultural biology, can solve the problems of long tissue culture cycle, high pollution rate, easy to cause mutation, etc., and achieve the effect of low production cost, high multiplication multiple, and maintaining excellent traits

Inactive Publication Date: 2013-04-17
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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Problems solved by technology

By comparison, the pollution rate of explants using stem segments is higher than that of explants using seeds, and th

Method used

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Experimental program
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Embodiment approach

[0024] 1. Explant Selection and Sterilization Process

[0025] Take mature black seeds of cattle, soak them in 0.05% detergent solution by volume for 10 minutes, and then wash them with running water for 15 minutes. Use 0.1% HgCl on the ultra-clean bench 2 The solution was sterilized for 16 minutes, then shaken and washed with sterile water for 4 times, then blotted dry with sterile absorbent paper, and then inoculated into the primary induction medium. The contamination rate of this sterilization method is 13.33%, and the survival rate is 100.00%.

[0026] If the fruit pods are not cracked, take the mature black fruit pods and soak them in 0.05% detergent solution for 10 minutes. During the soaking process, use a brush to scrub the surface to remove surface pubes and dirt, and then rinse them with running water for 15 minutes. Use 0.1% HgCl on the ultra-clean bench 2 The solution was sterilized for 20 minutes, then shaken and washed with sterile water for 3 times, and the ...

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Abstract

The invention discloses a method for directly inducing cluster buds of milletia speciosa champ seeds and quickly breeding seedlings. According to the method, cluster buds of milletia speciosa champ seeds are directly induced by an explant; and the method for breeding seedlings comprises explant sterilization, primary induction, secondary multiplication, rooting and transplanting to obtain milletia speciosa champ seedlings. The method disclosed by the invention has the advantages of simplicity and convenience in operation, low production cost, fast multiplication and high survival rate of rooting and transplanting, facilitates large-scale industrial production and application, can quickly and continuously obtain a great quantity of high-quality milletia speciosa champ test-tube seedlings, and has high practicability.

Description

technical field [0001] The invention belongs to the technical field of agricultural biology, and in particular relates to a method for directly inducing clustered buds and rapidly breeding seedlings from Niu Dali seeds. Background technique [0002] Niu Dali is a plant of the genus Millettia speciosa Champ. in the family Fabaceae, also known as Dali potato and mountain lotus root. ) local medicinal materials. The root of Niu Dali is used as medicine, which is sweet and flat. Return lung, kidney channel. It has the effect of tonifying deficiency and nourishing the lungs, strengthening tendons and activating collaterals. It has been clinically proven to have good curative effect on chronic diseases such as lumbar muscle strain, rheumatoid arthritis, lung deficiency cough, tuberculosis, chronic bronchitis, and chronic hepatitis. The folks of the Zhuang people are often used for the treatment of lumbago and leg pain, fever (rheumatic bone pain), tuberculosis, chronic hepatiti...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 姚绍嫦白隆华马小军
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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