Preparation technology of high-transparency squid skin collagen

A technology with high transparency and preparation process, applied in the direction of fermentation, etc., can solve the problems of complicated operation of the preparation method of squid skin collagen, dark color of squid skin collagen, light color of squid skin collagen, etc., and achieves easy industrial production, low collagen loss rate, good stability

Active Publication Date: 2013-04-24
ZHEJIANG MARINE DEV RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the disadvantages of complex operation of the squid skin collagen preparation method in the prior art, and the dark color of the obtained squid skin collagen, and provide a high-transparency squid skin collagen preparation process with simple operation and low processing cost. Squid skin collagen has light color, wide application range and high economic value

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A preparation process for high-transparency squid skin collagen, comprising the following steps:

[0032] a) Degreasing: Clean the squid skin with tap water, cut it into 4mm×4mm pieces, place it in a n-butanol solution with a volume fraction of 10%, and degrease it at 4°C for 24 hours. The amount of the solution is 3ml, and then rinsed 3 times with pure water;

[0033] b) Impurity protein removal: place the squid skin after rinsing in 0.04mol / L NaOH solution, soak at 4°C for a total of 16 hours, and replace it once with fresh NaOH solution once soaked for 8 hours. The dosage is 40ml;

[0034] c) Collagen extraction: Drain the squid skin after removing impurities, put it into pure water 10 times the mass of the squid skin, adjust the pH to 7.8, add trypsin with 0.08% squid skin mass, and enzymolyze it at 52°C for 120min. Collagen extraction stock solution was obtained after coarse filtration with gauze, and the collagen extraction stock solution was inactivated in a wa...

Embodiment 2

[0041] A preparation process for high-transparency squid skin collagen, comprising the following steps:

[0042] a) Degreasing: Clean the squid skin with tap water, cut it into 4mm×4mm pieces, place it in a n-butanol solution with a volume fraction of 10%, and degrease it at 6°C for 20 hours. The amount of the solution is 5ml, and then rinsed twice with pure water;

[0043]b) Impurity protein removal: put the rinsed squid skin in 0.06mol / L NaOH solution, soak at 6°C for a total of 12 hours, and replace it with fresh NaOH solution once when soaking for 6 hours, every 1g of squid skin NaOH solution The dosage is 30ml;

[0044] c) Collagen extraction: Drain the squid skin after removing impurities, put it into pure water with 12 times the mass of squid skin, adjust the pH to 8.2, add trypsin with 0.12% mass of squid skin, and enzymolyze it at 56°C for 90 minutes, using bag type The collagen extraction stock solution was obtained after filtering through a filter, and the collage...

Embodiment 3

[0051] A preparation process for high-transparency squid skin collagen, comprising the following steps:

[0052] a) Degreasing: Clean the squid skin with tap water, cut it into 4mm×4mm pieces, place it in a n-butanol solution with a volume fraction of 10%, and degrease it at 4.5°C for 22 hours. The amount of alcohol solution is 4ml, then rinse with pure water 3 times;

[0053] b) Impurity protein removal: put the rinsed squid skin in 0.05mol / L NaOH solution, soak at 5°C for a total of 14h, and replace it with fresh NaOH solution once when soaking for 6 hours, every 1g of squid skin NaOH solution The dosage is 35ml;

[0054] c) Collagen extraction: Drain the squid skin after removing impurities, put it into pure water 10 times the mass of squid skin, adjust the pH to 7.8, add trypsin with 0.1% squid skin mass, and enzymatically hydrolyze at 54°C for 100min. Collagen extraction stock solution was obtained after coarse filtration with gauze, and the collagen extraction stock so...

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Abstract

The invention relates to a preparation technology of high-transparency squid skin collagen, and aims at solving the defects of the quid skin collagen preparation method in the prior art that the operation is complicated and the prepared squid skin collagen is of dark color. The preparation technology comprises the following steps of: a) degreasing; b) eliminating impure protein; c) extracting the collagen: adding the squid skin without impure protein into the pure water of which the mass is 10-12 times that of the squid skin, regulating pH to 7.8-8.2, adding trypsase of which the mass is 0.08-0.12% of that of the squid skin at 52-56 DEG C, hydrolyzing for 90-120min, and executing enzyme deactivation for the collagen extract stock solution, obtained by coarse filtration, in water bath at 95-100 DEG C for 8-12min; d) decoloring: regulating pH of the collagen extract stock solution to 8.0-8.2, adding chelating agent and stirring evenly, further adding hydrogen peroxide of which the mass is 0.6-0.8% of that of the collagen extract stock solution, at 50-55 DEG C, decoloring 8-10h, continuously stirring during the decoloring process, and finally adding the manganese dioxide to remove the residual hydrogen peroxide; e) drying. The preparation technology is simple in operation and low in processing technology, and the prepared collagen is high in transparency, light in color and high in economic value.

Description

technical field [0001] The invention relates to a collagen preparation process, in particular to a high-transparency squid skin collagen preparation process. Background technique [0002] Collagen is a kind of fibrous protein, which accounts for about 30% of all proteins in animals. Its molecular composition is characterized by several Gly-X-Y sequence repeats and a unique triple helical structure composed of three polypeptide chains. Collagen and its degradation The product has a wide range of applications in chemical industry, food, medicine, biomaterials and agriculture due to its good physical properties and biological characteristics. my country is rich in squid resources, squid skin accounts for about 10% of the by-products in the squid deep processing process, and it contains about 80% collagen. The current extraction technology of squid skin collagen includes alkaline extraction, acid extraction and enzyme extraction, but due Squid skin collagen contains a certain am...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 付万冬廖妙飞章耀周枫季建杨会成
Owner ZHEJIANG MARINE DEV RES INST
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