Preparation technology of high-transparency squid skin collagen
A technology with high transparency and preparation process, applied in the direction of fermentation, etc., can solve the problems of complicated operation of the preparation method of squid skin collagen, dark color of squid skin collagen, light color of squid skin collagen, etc., and achieves easy industrial production, low collagen loss rate, good stability
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Embodiment 1
[0031] A preparation process for high-transparency squid skin collagen, comprising the following steps:
[0032] a) Degreasing: Clean the squid skin with tap water, cut it into 4mm×4mm pieces, place it in a n-butanol solution with a volume fraction of 10%, and degrease it at 4°C for 24 hours. The amount of the solution is 3ml, and then rinsed 3 times with pure water;
[0033] b) Impurity protein removal: place the squid skin after rinsing in 0.04mol / L NaOH solution, soak at 4°C for a total of 16 hours, and replace it once with fresh NaOH solution once soaked for 8 hours. The dosage is 40ml;
[0034] c) Collagen extraction: Drain the squid skin after removing impurities, put it into pure water 10 times the mass of the squid skin, adjust the pH to 7.8, add trypsin with 0.08% squid skin mass, and enzymolyze it at 52°C for 120min. Collagen extraction stock solution was obtained after coarse filtration with gauze, and the collagen extraction stock solution was inactivated in a wa...
Embodiment 2
[0041] A preparation process for high-transparency squid skin collagen, comprising the following steps:
[0042] a) Degreasing: Clean the squid skin with tap water, cut it into 4mm×4mm pieces, place it in a n-butanol solution with a volume fraction of 10%, and degrease it at 6°C for 20 hours. The amount of the solution is 5ml, and then rinsed twice with pure water;
[0043]b) Impurity protein removal: put the rinsed squid skin in 0.06mol / L NaOH solution, soak at 6°C for a total of 12 hours, and replace it with fresh NaOH solution once when soaking for 6 hours, every 1g of squid skin NaOH solution The dosage is 30ml;
[0044] c) Collagen extraction: Drain the squid skin after removing impurities, put it into pure water with 12 times the mass of squid skin, adjust the pH to 8.2, add trypsin with 0.12% mass of squid skin, and enzymolyze it at 56°C for 90 minutes, using bag type The collagen extraction stock solution was obtained after filtering through a filter, and the collage...
Embodiment 3
[0051] A preparation process for high-transparency squid skin collagen, comprising the following steps:
[0052] a) Degreasing: Clean the squid skin with tap water, cut it into 4mm×4mm pieces, place it in a n-butanol solution with a volume fraction of 10%, and degrease it at 4.5°C for 22 hours. The amount of alcohol solution is 4ml, then rinse with pure water 3 times;
[0053] b) Impurity protein removal: put the rinsed squid skin in 0.05mol / L NaOH solution, soak at 5°C for a total of 14h, and replace it with fresh NaOH solution once when soaking for 6 hours, every 1g of squid skin NaOH solution The dosage is 35ml;
[0054] c) Collagen extraction: Drain the squid skin after removing impurities, put it into pure water 10 times the mass of squid skin, adjust the pH to 7.8, add trypsin with 0.1% squid skin mass, and enzymatically hydrolyze at 54°C for 100min. Collagen extraction stock solution was obtained after coarse filtration with gauze, and the collagen extraction stock so...
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