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Suspension cell dyeing device

A staining device and a technique for suspending cells, which are applied in the fields of immunology and biology, can solve the problems of difficult operation of cell staining methods and obstacles to flow cytometry, and achieve the effects of saving requirements, improving accuracy, and overcoming experimental errors

Inactive Publication Date: 2013-04-24
WUHAN CELL MARKER & MACHINE TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Difficulties in the operation of cell staining methods have seriously hindered the application of cell detection equipment such as flow cytometry

Method used

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  • Suspension cell dyeing device
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  • Suspension cell dyeing device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] figure 1 Shown is the structure of a specific embodiment of the suspension cell staining device of the present invention. The suspension cell staining device is composed of a sleeve 1, a push rod 2, a plunger gasket 3, and a retaining membrane 4. The sleeve 1 is made of plastic or stainless steel. The casing 1 is a hollow circular tubular structure, and the plunger seal 3 is a circular sheet structure, which is located in the middle and upper part of the cavity of the casing 1. Sliding sealing fit, the push rod 2 is a long rod-shaped structure, its front end is connected with the upper part of the plunger gasket 3, and is integrated with the plunger gasket 3, and the rear end of the push rod 2 is provided with a handle that is easy for the operator to grasp. The push rod handle 7, the intercepting membrane 4 is located at the lower part of the casing 1 cavity, and a film opening 5 for injecting and discharging the solution is provided on the side wall of the casing 1 ab...

Embodiment 2

[0049] Combined suspension cell staining device, that is, the combined use of suspension cell staining devices.

[0050] The suspension cell staining device provided by the present invention can be used in combination, and two or more suspension cell staining devices can be used in combination, so that more cells can be treated at the same time, that is, more cells can be stained at the same time.

[0051] figure 2 An embodiment for the combined use of 4 suspension cell staining devices, the specific combination method is: a connecting pipe 10 is arranged below the 4 suspension cell staining devices arranged in parallel, and the solution on the outer tube 1 of the 4 suspension cell staining devices is injected into The lower opening 6 of the film and the discharge channel is connected to the communication pipe 10, and the two ends of the communication pipe 10 are provided with a communication pipe opening 11, and the opening at one end is provided with a flow joint 12, and a ...

Embodiment 3

[0054] Immunofluorescence staining experiment of cells using the suspension cell staining device prepared in Example 1

[0055] Experimental procedure: see figure 1

[0056] 1. Adding cells: open the valve 9, inject the suspension cells to be stained fixed in 70% ethanol into the cavity on the upper part of the retention membrane 4 in the casing 1, and close the valve 9 after injection;

[0057] 2. Wash cells:

[0058] a. Open the valve 8, pressurize and move the pushrod gasket to the direction of the intercepting membrane 4, so that the ethanol in the upper cavity of the intercepting membrane 4 passes through the intercepting membrane 4, and then injects and discharges the solution through the opening 6 under the sleeve membrane to form the valve One of the channels of the three-way valve of 8 is discharged, and the valve channel is closed after discharge. At this time, the cells to be stained are trapped on the retention membrane 4 .

[0059] b. Use a peristaltic pump to...

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PUM

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Abstract

The invention discloses a suspension cell dyeing device, which consists of a casing pipe (1), a push rod (2), a plunger sealing gasket (3), and an interception membrane (4). The plunger sealing gasket (3) is located at the upper-middle part in the casing pipe (1) cavity. The interception membrane (4) is positioned at the lower part in the casing pipe (1) cavity. The pipe wall of the casing pipe (1) is provided with an opening (5) above the membrane and an opening (6) below the membrane. Used for suspension cell dyeing, the device can effectively overcome the disadvantages of high failure rate, poor accuracy and repeatability, long time and the like in existing cell dyeing methods, and has the advantages of high success rate, good accuracy and repeatability, and short time, etc.

Description

technical field [0001] The invention belongs to the fields of immunology technology and biology technology, relates to cell staining technology, in particular to a suspended cell staining device. Background technique [0002] Cytology is the most commonly used technique in cell biology. Immunological staining assays for suspension cells have always used centrifugation to wash the cells. The steps are: obtain single suspension cells; fix cells or not fix cells; add antibodies (or other substances that can bind to cell markers); centrifuge to wash away unbound antibodies; add chromogenic substances; Color substances; detection of staining. According to various detection methods such as antibodies or anti-antibodies added, the above operation steps are basically repeated to complete cell immunostaining and finally detect cell markers. [0003] The detection of cell membrane markers by conventional methods is still barely feasible, but it is very difficult to detect intracell...

Claims

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Application Information

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IPC IPC(8): G01N1/31
Inventor 封江南
Owner WUHAN CELL MARKER & MACHINE TECH