Extraction method of glycyrrhiza polysaccharide and application

A technology of licorice polysaccharides and extraction methods, which is applied in the fields of application, tobacco, and tobacco preparation, can solve the problems of underutilized licorice residue, reduce cigarettes, and environmental pollution, and achieve high-value utilization, easy industrialization, and process The effect of simple process

Inactive Publication Date: 2013-05-01
乌鲁木齐天格尔农业科技开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For a long time, industrial production has only extracted glycyrrhizin (also known as glycyrrhizic acid) in licorice as the main active ingredient, and the residue of licorice after extracting glycyrrhizic acid has been discarded as waste, causing environmental pollution and waste of resources.
Due to the limitation of processing technology, licorice residue has not been fully utilized in my country at present, either used for breeding feed, or directly buried back to the land as fertilizer
At present, there is no report on the preparation of functional licorice polysaccharides that can reduce the cytotoxicity of tobacco condensate (CSC) in cigarettes by using licorice residue or licorice as raw materials

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) The licorice dregs are desanded and washed, dried and crushed, and the licorice dregs are obtained through a 40-mesh sieve;

[0030] (2) Add water 35 times the weight of the raw licorice residue, extract at 110°C for 5 hours, filter and separate the licorice residue to obtain the extract, and adjust the pH of the extract to 7.0 with potassium hydroxide;

[0031] (3) The extract obtained in step (2) is subjected to ultrafiltration with a membrane with a molecular weight cut-off of 5000 Dalton, and the intercepted liquid is collected;

[0032] (4) Concentrate the extract in vacuum to 1 / 5 of the original volume to obtain a concentrated solution;

[0033] (5) Add ethanol to the concentrated solution. After mixing, the final concentration of ethanol will be 70% (v / v). Let it stand at 2°C for 3 hours, and then filter to remove the supernatant and polysaccharide precipitation;

[0034] (5) Collect the precipitate and freeze-dry to obtain light yellow licorice residue polysaccharide ...

Embodiment 2

[0037] (1) The licorice dregs are desanded and washed, dried and crushed, and the licorice dregs are obtained through a 40-mesh sieve;

[0038] (2) Add citric acid solution with pH 2 at 35 times the weight of the licorice residue raw material, extract at 110°C for 5 hours, filter and separate the licorice residue to obtain the extract, and adjust the pH of the extract to 7.0 with potassium hydroxide ;

[0039] (3) The extract obtained in step (2) is subjected to ultrafiltration with a membrane with a molecular weight cut-off of 5000 Dalton, and the intercepted liquid is collected;

[0040] (4) Concentrate the intercepted liquid in vacuum to 1 / 5 of the original volume to obtain a concentrated liquid;

[0041] (5) Add ethanol to the concentrated solution. After mixing, the final concentration of ethanol will be 70% (v / v). Let it stand at 2°C for 3 hours, and then filter to remove the supernatant and polysaccharide precipitation;

[0042] (5) Collect the precipitate and freeze-dry to obta...

Embodiment 3

[0045] (1) The licorice dregs are desanded and washed, dried and crushed, and the licorice dregs raw materials can be obtained through 80 mesh sieve;

[0046] (2) Add citric acid solution with pH 1 to 20 times the weight of dry licorice residue, extract at 120°C for 2 hours, filter, separate the licorice residue to obtain the extract, adjust the pH of the extract to 6.5 with potassium hydroxide ;

[0047] (3) The extract obtained in step (2) is subjected to ultrafiltration with a membrane with a molecular weight cut-off of 5000 Dalton, and the intercepted liquid is collected;

[0048] (4) Concentrate the ultrafiltration permeate in vacuum to 1 / 5 of the original volume to obtain a concentrated solution;

[0049] (5) Add ethanol to the concentrated solution. After mixing, the final concentration of ethanol will be 85% (v / v). Let stand at 4°C for 12 hours, filter to remove the supernatant, and polysaccharide precipitation, and use ethanol and acetone. Dip 2-3 times;

[0050] (6) Collect ...

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PUM

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Abstract

The invention discloses an extraction method of glycyrrhiza polysaccharide and application. The glycyrrhiza polysaccharide is prepared by combining a high temperature organic acid method and ultrafiltration membrane treatment. The method comprises the steps of: smashing liquorice, and extracting with high temperature organic acid, ultrafiltration intercepting, concentrating and precipitating and separating by ethanol to obtain glycyrrhiza polysaccharide. The glycyrrhiza polysaccharide prepared by the invention is added to cigarettes by 0.05-0.2% of weight of cut tobacco, and the human bronchus epithelium BEAS-2B cell viability of a tobacco agglutinator of cigarettes obtained is remarkably higher than that of a blank sample, so that the glycyrrhiza polysaccharide has a positive effect.

Description

technical field [0001] The invention relates to the deep processing technology of licorice residue, in particular to the extraction method and application of licorice polysaccharide. Background technique [0002] With the continuous improvement of people's living standards and quality of life, cigarette consumption has shifted to brand consumption, especially in the high-end cigarette market. Consumers have higher and higher requirements for the health and flavor of cigarettes. The production of low-tar and low-harm cigarettes has become one of the important directions for the development of the cigarette industry in my country and even in the world. At present, there are two main technical routes to reduce the harm of cigarettes: one is "tar reduction", that is, the harmful components are reduced at the same time by reducing the release of cigarette tar, that is, the synergistic reduction of harmful components in cigarette smoke; the other is "Harm reduction" refers to the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A24B3/12A24B3/14
Inventor 田英姿赵谋明崔春英犁王希军
Owner 乌鲁木齐天格尔农业科技开发有限公司
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