Method for inducing corn haploid

A technology of haploid and haploid induction system, which is applied in the fields of botanical equipment and methods, application, plant gene improvement, etc., can solve the problems of unsatisfactory powder loosening and self-fertilization, unsatisfactory agronomic traits, etc. , to achieve good growth in the field, improve efficiency, and last for a long time

Active Publication Date: 2013-05-08
CHINA AGRI UNIV
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Problems solved by technology

However, although the induction rate of different newly selected induction lines has been greatly improved, the perfor

Method used

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  • Method for inducing corn haploid
  • Method for inducing corn haploid

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1, the performance of agronomic traits of different induced lines selfing and hybrid combinations

[0036] CAUHOI, UH400 and two newly selected induced lines CAU5 and CAU079 were selected. In Hainan in 2009, the above four lines were crossed with each other, and the contemporary grains (namely F1 generation) were harvested. In the summer of 2010, various F1 materials were planted at Changping Experimental Station in Beijing, and the agronomic traits of the parents and F1 generations were compared. Agronomic traits include: plant height, ear position, powder loosening period, silking period, etc.

[0037] Plant height: plant height refers to the distance from the ground to the top of the tassel of the plant;

[0038] Ear position: Ear height refers to the distance from the ground to the base of the ear;

[0039] Days from sowing to silking: the silking period refers to the silking period when the filaments of the pistil of the plant protrude from the bracts ...

Embodiment example 2

[0047] Implementation case 2. Comparison of induction rate differences between hybrids of different induction lines and their parents

[0048] The test was carried out at the Beijing Changping Test Station in the summer of 2010. Take Zhengdan 958 as the test species (as the female parent) to hybridize with the parental induced line and the induced line hybrid in the above-mentioned Table 1 respectively, and each induced line or induced line hybrid is hybridized with at least 10 ears of the female parent material; then harvest the hybrid Contemporary grains, threshing, and haploid identification were performed to compare the induction rate differences between parental induced lines and induced line hybrids.

[0049] The method of haploid identification is as follows:

[0050] (1) Classify and thresh the hybrid ears of Zhengdan 958 according to different paternal sources for haploid identification;

[0051] (2) Observe the color of hybrid grain embryo and endosperm aleurone la...

Embodiment example 3

[0066] Implementation case 3. Induction effect of inducible hybrid UH400 / CAU5 and different female parent materials

[0067] In the winter of 2009, haploid induction was carried out at the Hainan Experimental Station with two inducible lines, UH400 and CAU5.

[0068] In the summer of 2010, UH400 / CAU5 was used as the male parent to cross with different female parents. Three commercial hybrids were initially selected as the female parent for hybridization, and the induction effect of the induced hybrids under different material backgrounds was compared.

[0069] Specific hybridization method: the male parent and the female parent are planted alternately, and the planting area is isolated from the surrounding area (at least 200 meters around without other corn planting), the female parent is emasculated before the male parent is scattered, and the male parent is detasseled by wind power. Pollen reaches the female parent, and pollination is achieved.

[0070] The different female...

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Abstract

The present invention discloses a method suitable for commercially inducing a corn haploid. The method comprises the following steps: carrying out hybridization of a corn haploid induction line I and a corn haploid induction line II to obtain a progeny recorded as an induction line hybrid, adopting the induction line hybrid as a male parent, carrying out hybridization of the induction line hybrid and a female parent material expected to obtain a haploid to harvest F0 hybridization kernel, and taking the haploid kernel to obtain the corn haploid, wherein the corn haploid induction line I and the corn haploid induction line II are different. Compared with the traditional method, the method of the present invention has the following characteristics that: on the basis of maintaining of a high induction rate, agronomy characteristics of the male parent (induction line hybridization combination) in the haploid induction is substantially increased, and especially a plant growth condition, a pollen disseminating amount, a pollen disseminating duration time, adaptability, resistance, and the like are substantially increased so as to improve haploid induction efficiency, provide a guarantee for efficient haploid induction, and provide possibility for large-scale haploid induction.

Description

technical field [0001] The invention relates to a method suitable for commercializing maize haploid induction. Background technique [0002] The selection of inbred lines is an important link in maize breeding, which almost determines the success or failure of hybrid breeding. Since maize is a monoecious and sexually reproductive crop, the sexual reproduction of maize is mainly used to select maize inbred lines, prepare maize combinations, and screen maize hybrids. The traditional breeding process of maize inbred lines must go through many years of self-crossing and selection. It usually takes ten years to breed a variety, which not only takes a long period, but also consumes a lot of manpower, material and financial resources. Therefore how to shorten the breeding process and improve the breeding efficiency is the goal that breeders have been pursuing. [0003] As a high-tech, haploid technology brings broad prospects for breeders to quickly select and select inbred lines...

Claims

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Application Information

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IPC IPC(8): A01H1/02
Inventor 陈绍江黎亮徐小炜李浩川
Owner CHINA AGRI UNIV
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