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Method for subculturing provenance of enoki mushrooms

A white enoki mushroom and provenance technology, which is applied in the field of white enoki mushroom provenance, can solve the problems of rapid degradation of introduced or isolated strains, and achieve the effects of solving strain degradation, prolonging the number of passages, and uniform growth speed of strains

Active Publication Date: 2013-05-22
辽宁天赢生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved in the present invention is to provide a method for the provenance of Flammulina velutipes, which can solve the problem of rapid degradation of introduced or isolated strains, and ensure that good strains can be kept for a long time under the means of normal temperature passaging genetic and production stability

Method used

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  • Method for subculturing provenance of enoki mushrooms
  • Method for subculturing provenance of enoki mushrooms

Examples

Experimental program
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Effect test

Embodiment 1

[0015] 1) make slant medium: the provenance subculture of white Flammulina velutipes takes test tube subculture as main means, adopts potato medium (PDA) as culture substrate, culture medium: mix in the weight ratio of water=1: 2.5, heat up and dissolve, fully After dissolving, it is divided into ￠18mm test tubes, each test tube is filled with 12ml of medium, sterilized at 121°C for 30 minutes, cooled to 45°C after sterilization, and placed in a slant to cool to make a slant medium.

[0016] 2) Inoculation and cultivation: Take 2mm*2mm*2mm parent bacterial block under the ultra-clean bench, put it at the bottom 3cm of the test tube, plug it with a silica gel plug, and cultivate it at 17-18°C, on the 9th and 14th days Select once every day, and record the growth rate of mycelium, eliminate bad strains, and the subculture cycle is 14 days;

[0017] 3) Passage comparison and selection: the inoculated test tubes were divided into 4 lines for parallel passage, and each generation w...

Embodiment 2

[0020] 1) make slant medium: the provenance subculture of white Flammulina velutipes takes test tube subculture as main means, adopts potato medium (PDA) as culture substrate, culture medium: mix in the weight ratio of water=1: 2.5, heat up and dissolve, fully After dissolving, divide into ￠18mm test tubes, each test tube contains 16ml of culture medium, sterilize at 121°C for 15 minutes, cool down to 45°C after sterilization, take it out of the pot, place it on a slope to cool, and make a slope medium.

[0021] 2) Inoculation and cultivation: take a 5mm*5mm*3mm parent bacterial block under the ultra-clean bench, put it at the bottom 4cm of the test tube, plug it with a silica gel plug, and carry out cultivation at 19-20°C. They were selected every 6 days and 12 days respectively, and the subculture cycle was 12 days.

[0022] 3) Passage comparison and selection: Test tube passage adopts a 6-way system in parallel, and abnormal strains are eliminated at each selection, includi...

Embodiment 3

[0025] 1) make slant medium: the provenance subculture of white Flammulina velutipes takes test tube subculture as main means, adopts potato medium (PDA) as culture substrate, culture medium: mix in the weight ratio of water=1: 2.5, heat up and dissolve, fully After dissolving, it is divided into ￠18mm test tubes, each test tube is filled with 14ml of culture medium, sterilized at 121°C for 20 minutes, cooled to 45°C after sterilization, taken out of the pot, placed on a slant to cool, and made into a slant medium.

[0026] 2) Subculture inoculation: Take a 2mm*2mm*3mm parent bacterial block under the ultra-clean bench, put it at the bottom 2cm of the test tube, plug it with a silica gel plug, and culture at 18-19°C. They were selected every 8 days and 13 days respectively, and subcultured every 13 days, that is, the subculture cycle was 13 days.

[0027] 3) Selection: Test tube subculture adopts a 6-way system in parallel, and eliminates abnormal strains at each selection, in...

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Abstract

The invention discloses a method for subculturing provenance of enoki mushrooms. The method includes subculturing the provenance of the enoki mushrooms by the aid of test tubes and PDA (potato dextrose agar) slant culture media, culturing the provenance at the temperature of 16-20 DEG C, respectively selecting mycelia and recording the growth rate of the mycelia on the 6-9th day and the 11-14th day and eliminating unhealthy strains; grouping the test tubes into 4-10 channels after subculturing, performing parallel subculturing for the 4-10 channels of test tubes, transversely comparing each generation, eliminating a certain channel when strains in the certain channel are continuously at abnormal growth conditions twice, and selecting the strains at the most stable growth conditions for subculturing; and eliminating a system of a certain channel when the strains of the system of the certain channel degenerate twice and account for 40% of the total strains of the system of the certain channel, selecting the channel with the highest quality rate in the 4-10 channels for supplement, and continuing performing parallel subculturing for systems of the 4-10 channels. The subculturing cycle ranges from 11 to 14 days. The method has the advantages that the problem of strain degeneration due to subculturing can be solved, the subculturing frequency of the strains is effectively increased, and excellent characteristics of the strains can be kept for a plurality of years.

Description

technical field [0001] The invention relates to the technical field of production methods of parent species of edible fungi, in particular to a method for the generation of white Flammulina velutipes. Background technique [0002] White Flammulina velutipes is a white variant (F.var.velutipes) of Flammulina velutipes, scientific name is Enoki Mushroom, and Latin literary name is Flammulina velutiper (Fr.) Sing. Bacteriaceae, Tricholomaceae, Flammulina or Collybia. Now Japan, South Korea, China, and China Taiwan all have relatively large-scale cultivation, and domestically, it is mainly cultivated and produced in factories after domestication from Japan. [0003] The source of the mother species of Flammulina velutipes is mostly obtained by introduction and tissue separation, and the mother species is mostly subcultured on potato agar medium, which is subcultured every 7-10 days, and the culture temperature is 20-25°C. Due to the fluctuation of medium nutrition, nutrient im...

Claims

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Application Information

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IPC IPC(8): A01G1/04
Inventor 刘汉席
Owner 辽宁天赢生物科技股份有限公司
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