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Method, system and kit for detecting urinary microalbumin

A urine microalbumin and kit technology, applied in chemical instruments and methods, fluorescence/phosphorescence, organic dyes, etc., can solve problems such as difficulty in promotion, pollution of the environment, and impact on the health of operators

Active Publication Date: 2016-12-28
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, radioimmunity uses radioactive isotopes as tracers, which affects the health of operators and seriously pollutes the environment
Other detection methods also have the characteristics of being difficult to popularize due to the limitations of instrument and reagent conditions.

Method used

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  • Method, system and kit for detecting urinary microalbumin
  • Method, system and kit for detecting urinary microalbumin
  • Method, system and kit for detecting urinary microalbumin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] In this example, the urine microalbumin concentration of three urine samples is verified. The actual urine microalbumin concentration of each urine sample is as follows: urine sample 1 is 5.84 mg / L, urine sample 2 is 22.25 mg / L, urine sample 2 is 22.25 mg / L, and urine sample 2 is 22.25 mg / L. Sample 3 was 68.17mg / L. The cyanine dye used in this example is a compound of the following formula

[0066]

[0067] 1) Prepare standard solution samples

[0068] A certain amount of urinary microalbumin was dissolved in Tris buffer (pH 8.0, buffer 1 mmol / L) containing 150 mmol / L NaCl to prepare a urine albumin mother solution with a concentration of 1 g / L for later use.

[0069] Take 200 μL of cyanine dye methanol solution with a concentration of 200 μmol / L, and add 9.8 ml of Tris buffer (pH 8.0, buffer 1 mmol / L) containing 20 mmol / L NaCl. Divide the above sample into 10 parts on average, each sample solution is 1mL.

[0070] Take 7 samples among them, add a certain amount o...

Embodiment 2

[0080] In this example, the urine microalbumin concentration of three urine samples is verified. The actual urine microalbumin concentration of each urine sample is as follows: urine sample 1 is 9.18mg / L, urine sample 2 is 32.43mg / L, urine sample 2 is 32.43mg / L, urine Sample 3 was 149.57mg / L. The cyanine dye used in this example is a compound of the following formula

[0081]

[0082] 1) Prepare standard solution samples

[0083]A certain amount of urinary microalbumin was dissolved in sodium phosphate buffer solution (pH 5.0, buffer concentration 45 mmol) containing 150 mmol / L NaCl to prepare a urine albumin mother solution with a concentration of 1 g / L for later use.

[0084] Take 200 μL of cyanine dye methanol solution with a concentration of 200 μmol / L, and add 9.6 ml of sodium phosphate buffer (pH5.0, 45 mmol) containing 10 mmol / L NaCl. Divide the above sample into 10 parts on average, each sample solution is 0.98mL.

[0085] Take 7 samples, add a certain amount of ...

Embodiment 3

[0095] In this example, the urine microalbumin concentration of three urine samples is verified. The actual urine microalbumin concentration of each urine sample is as follows: urine sample 1 is 9.82 mg / L, urine sample 2 is 20.35 mg / L, urine sample 2 is 20.35 mg / L, urine Sample 3 was 78.26mg / L. The cyanine dye used in this example is a compound of the following formula

[0096]

[0097] 1) Prepare standard solution samples

[0098] Dissolve a certain amount of urinary microalbumin in triethanolamine buffer (pH 7.0, buffer concentration 30mmol / L) containing 150mmol / L NaCl and 30mmol / L KCl to prepare a urine albumin stock solution with a concentration of 1g / L ,spare.

[0099] Take 2 mL of cyanine dye methanol solution with a concentration of 200 μmol / L, and add 6 mL of triethanolamine buffer solution (pH 7.0, buffer concentration 30 mmol). Divide the above sample into 10 parts on average, each sample solution is 0.8mL.

[0100] Take 7 of the samples, add a certain amount ...

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Abstract

The invention relates to a detection method, a system and a kit of microalbuminuria. The method for detecting the concentration of microalbuminuria in a solution comprises the following steps of: (1) preparing a plurality of solution samples with different concentrations of microalbuminuria, wherein each solution sample contains cyanine dyes with same concentration; (2) detecting fluorescence intensity values of an acquisition wavelength part; (3) obtaining a standard curve of the microalbuminuria concentration; (4) adding the cyanine dyes and a buffer solution containing potassium ions or sodium ions in a liquid sample to be detected, so that the concentration and the pH value of the cyanine dyes in the liquid solution to be detected is consistent with those of the solution sample in the step (1), so as to obtain a test solution and record a dilution ratio of the liquid sample to be detected; (5) detecting the fluorescence intensity values of the acquisition wavelength part; and (6) finding the microalbuminuria concentration value of the corresponding test solution in the standard curve, and calculating the microalbuminuria concentration of the sample to be detected, wherein the acquisition wavelength is in the range of 550 to 650 nanometers.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a urine microalbumin detection method, system and kit. Background technique [0002] Microalbuminuria is the presence of tiny amounts of albumin in the urine. Albumin is a normal protein in the blood, but only very small amounts are present in the urine under physiological conditions. Microalbuminuria reflects abnormal protein leakage from the kidneys. Increased urinary microalbumin is more common in diabetic nephropathy, hypertension, and preeclampsia, and is an early sensitive indicator of kidney damage. Urinary microalbumin indicators are usually used to monitor the occurrence of nephropathy. The detection of urinary microalbumin is the most sensitive and reliable diagnostic index for early detection of nephropathy. [0003] Urinary microalbumin is used to evaluate the degree of kidney damage: when the urine microalbumin is found to be in the range of 20mg / L-200mg / L,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCC09B23/06C09B23/0008
Inventor 孙红霞唐亚林杨千帆管爱娇
Owner INST OF CHEM CHINESE ACAD OF SCI
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