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NMR (nuclear magnetic resonance) quick detection method for food-borne pathogens based on Fe3O4@Au composite nano particles

A technology of composite nanoparticles and food-borne pathogenic bacteria, which is applied in the direction of measuring devices, instruments, and analysis using nuclear magnetic resonance, etc., can solve the problems of not using nuclear magnetic resonance, etc.

Inactive Publication Date: 2013-05-22
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no literature reporting this method at home and abroad, but there are still many reports on the enrichment of pathogenic bacteria and target objects using immunomagnetic beads, but none of them have used nuclear magnetic resonance for further detection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0020] Testing food samples to determine whether they contain the harmful pathogenic bacterium Listeria.

[0021] 1. Preparation of immunomagnetic beads:

[0022] Synthesis of Fe with core-shell structure by "two-step method" 3 o 4 / Au nanoparticles. First the iron salt [n(Fe 3＋ ) / n(Fe 2＋ )=2 / 1.2] mixed evenly, placed in a constant temperature bath at 50°C, added 2 mol / L NaOH under stirring, and controlled the pH of the solution to be about 11, until the pH remained unchanged, then raised the temperature to 80°C and matured for 1 hour, the whole reaction Carried out under nitrogen protection. The precipitate was separated by magnetic force and washed several times with double distilled water to neutrality to obtain Fe 3 o 4 Suspension, constant volume and determination of its solid content. Take the right amount of Fe 3 o 4 Seed suspension with HAuCl 4 The solution was mixed for 1h, then an excess of hydroxylamine hydrochloride (80mmol / L) was added, and the reaction...

example 2

[0031] Determination of food samples for the presence of the harmful pathogen Escherichia coli O157:H7.

[0032] 1. Using a "two-step method" to synthesize Fe with a core-shell structure 3 o 4 Au composite nanoparticles. First the iron salt [n(Fe 3＋ ) / n(Fe 2＋ )=2 / 1.2] mix evenly, place in a constant temperature tank at 50°C, add 2 mol / L NaOH under stirring, control the pH of the solution to about 11, until the pH remains unchanged, then raise the temperature to 80°C and mature for 1 hour, the whole reaction Carried out under nitrogen protection. The precipitate was separated by magnetic force and washed several times with double distilled water to neutrality to obtain Fe 3 o 4 Suspension, constant volume and determination of its solid content. Take the right amount of Fe 3 o 4 Mix the seed suspension with the HAuCl4 solution for 1 hour, then add excess hydroxylamine hydrochloride (80 mmol / L), and react for 1 hour under stirring to obtain Fe with core-shell structure. ...

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PUM

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Abstract

The invention relates to an NMR (nuclear magnetic resonance) quick detection method for food-borne pathogens based on Fe3O4@Au composite nano particles, belonging to the technical field of quick detection of food safety pathogens. Depending on an established NMR detection method which can be used for pathogens in a food liquid sample, the invention prepares immunomagnetic beads from Fe3O4@Au composite nano materials, enriches target strains in a targeted manner, and detects whether the target pathogens are contained in the sample through the influence of paramagnetic and superparamagnetic characteristics of the Fe3O4@Au composite nano particles on the NMR attenuation signal relaxation time. Different specific corresponding relationships are as follows: the paramagnetic immunomagnetic beads show a linear relationship under certain conditions, i.e. the higher the immunomagnetic bead content is, the smaller the T2 / T1 value of the sample is; and the target bacteria can be quantitatively detected in a certain range. The method can be used for the quick detection of harmful pathogens in a food sample, and thus can be used for quickly screening a large number of samples to be detected.

Description

technical field [0001] The invention relates to a rapid detection method for pathogenic bacteria, in particular to a method based on Fe 3 o 4 Rapid NMR detection of foodborne pathogens using Au composite nanoparticles. Background technique [0002] Basic principle: Monoclonal antibodies or antigen molecules are combined with enzyme molecules through covalent bonds. This combination will not change the immunological characteristics and biological activities of monoclonal antibodies, antigens and enzymes. Specific monoclonal antibodies will only interact with specificity. antigen binding. The main principle steps: 1. The magnetic Fe 3 o 4 Au core-shell nanoparticles are surface functionalized by modifying antibodies, and monoclonal antibodies are coupled to the surface of magnetic beads to form specific immunomagnetic beads and block redundant active sites. 2. Immobilize the specific monoclonal antibody of another part of the target bacteria on the surface of the solid ph...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N24/08G01N33/577
Inventor 张锦胜唐群赖卫华
Owner NANCHANG UNIV