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System for airborne bacterial sample collection and analysis

一种样品、收集器的技术，应用在颗粒的系统领域，能够解决没有满足提供等问题

Inactive Publication Date: 2013-05-22
DETON
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, a key challenge in providing a detection system with sufficient sensitivity is the collection and rapid amplification of low concentrations of bacterial aerosols by delivering highly concentrated analytes such as DNA to the sensor.
Conventional sensors simply do not meet the requirements needed to provide a direct diagnosis of possible infection or contamination risk from aerosol sources (such as a patient's exhalation, cough or sneeze) or from the environment

Method used

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  • System for airborne bacterial sample collection and analysis
  • System for airborne bacterial sample collection and analysis
  • System for airborne bacterial sample collection and analysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] Example 1: Comparison of Performance with Traditional Systems - Sampling of the Environment

[0079] Turning to a comparison of the operation of the present invention with that of conventional aerosol collection and analysis systems, it should first be understood that access to the interior components (IC) of cells, primarily DNA, is essential for most detection methods. (See eg N. Bao and C. Lu., Principles of Bacterial Detection: Biosensors, Recognition Receptors and Microsystems, pages 817-831. Springer, (2008), the disclosure of which is hereby incorporated by reference). In traditional biosensor systems, such as Figure 2a As shown in the block diagram, the bacterial aerosol is first collected in the first part (46), and then cracked in the second part (48) by a mechanical system, a heating system, a chemical system, an electrical system or a laser system. The cleavage product is then purified in the next section (not shown) to deliver the target molecule to a s...

Embodiment 2

[0097] Example 2: Study of cell rupture

[0098] Studies were carried out using the experimental apparatus described in Example 1 to show that airborne bacteria subjected to aerodynamic shock rupture by undergoing relative deceleration due to sudden changes in gas velocity. When χ=P 1 / P 0 1 is the pressure downstream of the nozzle, P 0 is the pressure upstream of the nozzle), an aerodynamic impact is generated by operating the impactor nozzle at sonic speed. Uncontrolled instability in the form of waves on the surface of the bacteria perpendicular to the direction of acceleration leads to the rupture of the bacterial cells. The critical acceleration ac is given by the following equation:

[0099] a c = 4 π 2 σ ρ p d p 2 ...

Embodiment 3

[0104] Example 3: Method for Measuring DNA Collection Efficiency

[0105] The experimental equipment provided by embodiment 1 can also be used to measure the collection efficiency (η d ). In such an experiment, a suspension of bacteria may be aerosolized using a capillary nebulizer (TR-30-A1, product of Meinhard Glass) at a nitrogen flow rate of 0.2 mL / min. Suspension concentration and flow rate will be controlled to produce single bacterial cells in the aerosol.

[0106] E. coli will be used as the test aerosol because (1) E. coli does not require a biosafety room and (2) E. coli is easy to grow and clean. Furthermore, S. pneumoniae is a plant-growing bacterium and is expected to have similar ac and thus f-values ​​as E. coli vs. B. atrophaeus spores. ds-DNA (double stranded) in our samples will be stained with PicoGreen fluorescent dye (P11495, Life Technologies) using procedures provided by the manufacturer and in other work. (See W. Martens-Habbena and H. Sass cited ...

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Abstract

An aerosol biological collector / analyzer, and method of collecting and analyzing an aerosol sample for diagnosis is provided. In particular, the current invention is directed to an airborne aerosol collection and bacterial analysis system and method, capable of collecting an airborne aerosol sample and preparing it for analysis via aerodynamic shock in a single-step.

Description

technical field [0001] The present invention relates to systems for the collection and analysis of airborne bacterial or biological particles; more particularly, to systems comprising an aerosol collection system for the detection of airborne bacterial or biological particles that Can be used to prepare samples for diagnosis or to directly diagnose bacterial infection samples from the environment or from patients such as, for example, tuberculosis. Background technique [0002] Tuberculosis (TB) is a contagious disease that kills 2 million people every year. Approximately 9.3 million people worldwide develop TB each year, of whom an estimated 4.4 million are undiagnosed. Improved diagnosis of TB could lead to saving approximately 625,000 annual adjusted lives saved worldwide, and eradicate TB from industrialized countries. (See, e.g., Center of Disease Control and Prevention, TB elimination: Trends in tuberculosis 2008, (2009); and WorldHealth Organization, Diagnostics for...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/22G01N31/00C12Q1/70C12N1/06
CPCG01N1/2208A61B5/097A61B5/082G01N2001/2217G01N33/497G01N2001/2244C12Q1/689
Inventor 帕特里克·西斯利安拉姆齐·纳斯尔马森·纳斯尔
Owner DETON
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