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Detection and analysis method for component containing salvianolic acid A

An analysis method, the technology of salvianolic acid, is applied in the field of detection and analysis containing salvianolic acid A component, which can solve the problems of high toxicity, disorder of the human respiratory system and circulatory system, high price of chromatographic acetonitrile, etc., and achieve the goal of ensuring accuracy Effect

Active Publication Date: 2013-05-29
JIANGZI QINGFENG PHARMACEUTICALS INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, acetonitrile itself is highly toxic, and long-term exposure and inhalation can cause disorders in the human respiratory system and circulatory system, and the chromatographic acetonitrile for analysis and detection is expensive

Method used

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  • Detection and analysis method for component containing salvianolic acid A
  • Detection and analysis method for component containing salvianolic acid A
  • Detection and analysis method for component containing salvianolic acid A

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Danshen herb detection

[0056] Chromatographic column; C 18 Reverse-phase chromatographic column, specification: 250×4.6mm, 5μm;

[0057] Chromatographic conditions and system suitability experiment: Octadecylsilane bonded silica gel as filler; flow rate 1.0ml / min; column temperature 30°C;

[0058] From 0 to 10 minutes, the proportion of methanol increased from 30% to 40%, and the proportion of 0.15% phosphoric acid aqueous solution decreased from 70% to 60%; from 10 to 30 minutes, the proportion of methanol increased from 40% to 55%, 0.15% The proportion of phosphoric acid aqueous solution is reduced from 50% to 45%; 30~60 minutes, the proportion of methanol rises from 55% to 80%, and the proportion of 0.15% phosphoric acid aqueous solution decreases from 45% to 20%.

[0059] Preparation of the reference solution: accurately weigh the reference substance of salvianolic acid A into a volumetric flask, add methanol to dissolve, shake well, and dilute to the mark;

[...

Embodiment 2

[0064] Detection and Analysis of Danshen Injection

[0065] Column: C 18 Reverse-phase chromatographic column, specification: 250×4.6mm, 5μm;

[0066] Chromatographic conditions and system suitability experiment: Octadecylsilane bonded silica gel as filler; flow rate 1.0ml / min; column temperature 30°C;

[0067] At 0 to -10 minutes, the ratio of methanol alcohol rose from 30% to 40%, and the ratio of 0.2% phosphoric acid aqueous solution dropped from 70% to 60%; at 10 to 30 minutes, the ratio of methanol rose from 40% to 55%. The proportion of 0.2% phosphoric acid aqueous solution decreased from 50% to 45%; in 30-60 minutes, the proportion of methanol increased from 55% to 80%, and the proportion of 0.2% phosphoric acid aqueous solution decreased from 45% to 20%.

[0068] Preparation of the reference solution: accurately weigh the reference substance of salvianolic acid A into a volumetric flask, add methanol to dissolve, shake well, and dilute to the mark;

[0069] Preparat...

Embodiment 3

[0073] Detection and Analysis of Salvianolic Acid A Sample

[0074] Column: C 18 Reverse-phase chromatographic column, specification: 250×4.6mm, 5μm;

[0075] Chromatographic conditions and system suitability experiment: Octadecylsilane bonded silica gel as filler; flow rate 1.0ml / min; column temperature 30°C;

[0076] From 0 to 10 minutes, the proportion of methanol increased from 30% to 40%, and the proportion of 0.25% phosphoric acid aqueous solution decreased from 70% to 60%; from 10 to 30 minutes, the proportion of methanol increased from 40% to 55%, 0.25% The ratio of phosphoric acid aqueous solution is reduced from 50% to 45%; 30~60 minutes, the ratio of methanol rises from 55% to 80%, the ratio of 0.25% phosphoric acid aqueous solution is reduced from 45% to 20%.

[0077] Preparation of the reference solution: accurately weigh the reference substance of salvianolic acid A into a volumetric flask, add methanol to dissolve, shake well, and dilute to the mark;

[0078]...

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Abstract

The invention relates to a detection and analysis method for a component containing salvianolic acid A. The component containing salvianolic acid A is selected from the medical material of salviae miltiorrhizae, the raw material of salvianolic acid A or pharmaceutic preparations containing the salvianolic acid A. According to the detection and analysis method, high performance liquid chromatography is utilized for detecting and analyzing, so that the accuracy of the content of the salvianolic acid A is ensured and the stability and the no degrading of the salvianolic acid A in the detection can be ensured. Meanwhile, the defects of serious toxicity, harm to human body and high price caused by overuse of acetonitrile are overcome.

Description

technical field [0001] The invention relates to a detection and analysis method containing salvianolic acid A component. Background technique [0002] Salvianolic acid A (Salvianolic acid A), also known as salvianolic acid A, is a water-soluble phenolic acid compound contained in the dry roots and rhizomes of Salviamiltiorrhiza Bunge, a plant of the Labiatae family. Embolism has obvious pharmacological effects, and the effect is better than that of salvianolic acid B (Zhang Hengai. The effect and mechanism of salvianolic acid A on cerebral microvascular embolism in rats [D]. Beijing: Graduate School of Peking Union Medical College, 2011], Salvianolic acid A is formed by the condensation of danshensu and caffeic acid. It contains multiple active groups such as phenolic hydroxyl groups, hydroxyl groups, and ester bonds. It is unstable to light and heat. It is easily oxidized into salvianolic acid C and isodan Due to the above physical and chemical properties of salvianolic ac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/89
Inventor 程帆曾发林刘鹏刘艳红罗恒真王章伟刘荛奇
Owner JIANGZI QINGFENG PHARMACEUTICALS INC
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