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Process to prepare liquid microbe organic fertilizer by means of animal blood enzymolysis and fermentation synchronous method

A technology of microbial organic fertilizer and synchronous method, applied in the field of bioengineering, can solve problems such as loss, organic wastewater pollution, environmental economy, and hidden dangers of feed safety, and achieve the effects of increasing soluble protein content, inhibiting reproduction, and high enzyme activity

Active Publication Date: 2013-06-05
李季芬
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  • Summary
  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0003] In order to solve the problems of hidden dangers of feed safety, environmental pollution and economic loss caused by organic waste water pollution and economic loss caused by corruption once the blood is used as the raw material for the production of feed additives in slaughterhouses in the process of smearing red, the present invention provides a A process for preparing liquid microbial organic fertilizer by synchronous method of enzymatic hydrolysis and fermentation of animal blood, which can improve the comprehensive utilization rate of slaughtering leftovers and help slaughtering enterprises maximize economic benefits

Method used

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Examples

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Embodiment Construction

[0014] The technical solution of the present invention will be described in detail below in conjunction with the examples, but it does not limit the protection scope of the present invention. Any changes or replacements that can be easily imagined by those skilled in the art within the technical scope disclosed in the present invention should be covered. within the protection scope of the present invention.

[0015] The present invention prepares liquid microbial organic fertilizer according to the following steps:

[0016] 1. Bacterial solution preparation:

[0017] 1.1 Lactobacillus rhamnosus liquid:

[0018] Medium: glucose 20.0g, casein peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, anhydrous sodium acetate 5.0g, dipotassium hydrogen phosphate 2.0g, diammonium hydrogen citrate 2.0g, MgSO4 7H 2 O 0.58g, MnSO 4 ·H 2 O 0.33g; add distilled water to 1000mL, pH 6.4, sterilize at 121°C for 20min.

[0019] Inoculate the activated Lactobacillus rhamnosus bacteria liqu...

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Abstract

The invention provides process to prepare liquid microbe organic fertilizer by means of an animal blood enzymolysis and fermentation synchronous method, and belongs to the technical field of biological engineering. In order to solve the problems that blood poured in a red-spreading working procedure in a slaughter house is used as raw materials for producing feed additives, once the blood goes bad due to untimely clearing and transportation, feed safety hidden risks are caused, organic waste water pollutes the environment and economic losses are caused, the process is raised. According to the process, animal blood (pig blood, cattle blood, goat blood, and donkey blood and the like) serves as main raw materials to ferment substrates, brown sugar, white sugar, protease, and soil beneficial microbe bacteria perform auxiliary functions, the leftover animal blood and the organic waste water in the slaughter house are degraded in the enzymolysis and fermentation synchronous technique, and the organic fertilizer is produced. Animal proteolytic enzyme is added for enzymolysis, Pichia jadinii yeast, bacillus licheniformis and lactobacillus rhamnosus composite viable bacteria are added for fermentation, and protein in the blood is completely degraded. According to the process, the comprehensive utilization rate of leftovers in slaughtering can be improved, and economic benefit maximization of a slaughter enterprise is aided.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and relates to a process for preparing liquid microbial organic fertilizer through a synchronous method of enzymolysis and fermentation of animal blood. Background technique [0002] China is a big meat production and consumption country in the world. In recent years, with the improvement of people's living standards, the demand for livestock and poultry food has increased year by year, which has led to the unprecedented development of the breeding industry. my country has a large amount of livestock and poultry slaughtered every year, and blood resources are very rich. In the past, the blood poured out of the slaughterhouse during the red smearing process was used as the raw material for the production of feed additives. Once it was not cleared and transported in time, corruption would cause hidden dangers in feed safety, environmental pollution by organic wastewater, and economic losses...

Claims

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Application Information

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IPC IPC(8): C05F11/00
CPCY02W30/40
Inventor 牟钰德赵思雨赵伟洁
Owner 李季芬
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