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Rapid propagation method for cactus

A cactus and rapid propagation technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of high cost and high survival rate, and achieve the effects of shortening the growth cycle, increasing the value-added rate and the rate of strong seedlings

Inactive Publication Date: 2013-06-12
王茜
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has high survival rate and high cost

Method used

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Experimental program
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Embodiment 1

[0021] The formula of the medium A is: 1 / 4MS, ZT 1mg / L, NAA 0.5mg / L, sugar 30g / L, agar 5g / L, pH 5.4.

[0022] The formula of the medium B is: 1 / 4MS, ZT 0.5mg / L, NAA 0.5mg / L, sugar 30g / L, agar 5g / L, pH is 5.6.

[0023] 1. Cut the healthy cactus that has grown for 1 year as explants; treat the explants with 75% alcohol for 5 seconds; then soak in 0.1% mercury chloride for 30 seconds, shake the bottle constantly; then rinse with sterile water 4 times , such a processing method has obtained a larger number of sterile explants and surviving individuals;

[0024] 2. Inoculate the sterilized explants on culture medium A in a sterile working environment, the culture conditions are 12 hours of light time per day, 1500 lx of light intensity, and 24° C. of culture temperature; two weeks after the inoculation of the explants, Stem tip buds begin to grow, side buds swell and germinate, and continue to cultivate until 45 days;

[0025] 3. Cut off the above-mentioned adventitious buds, ino...

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Abstract

The invention discloses a rapid propagation method for a cactus, which relates to a rapid propagation method for a plant and belongs to the technical field of agriculture. The method is characterized by comprising the following steps: cutting a healthy cactus at an age of 1 to 3 years as an explant; treating the explant with 75% alcohol for 5 s; soaking the explant with 0.1% mercuric chloride for 30 s, with a bottle being shook continuously; and carrying out flushing with sterile water four to five times. With the method, a great number of germ-free explants and survival individuals are obtained. The invention has the following beneficial effects: through packaged technologies of cottage rapid propagation close planting, artificial cultivation and trimming and the like, a growth cycle of the cactus is shortened; through usage of ABT rooting powder for acceleration of induced differentiation, a good effect of 100% of bud inductivity is obtained; through appropriate usage of the ABT rooting powder, an appreciation rate and a strong seedling rate of the cactus are improved, and an optimal rooting agent is screened.

Description

[0001] technical field [0002] The invention relates to a method for rapid propagation of plants, which belongs to the technical field of agriculture, in particular to a method for rapid propagation of cactus. Background technique [0003] The cactus (Cactaceae) is a plant and the national flower of Mexico. Belongs to a family of desert plants of the order Caryophyllaceae. In order to adapt to the water-scarce climate of the desert, the leaves evolved into short thorns to reduce water evaporation and also serve as weapons to prevent animals from swallowing; the stem evolved into a plump and water-containing shape; at the same time, it grew to cover a very large area The roots are used to absorb the most rainwater when it rains heavily. At present, there are nearly 2,000 species of plants in the cactus family. [0004] People often think that cacti are not useful as ornamental plants. Actually otherwise, the fruit of many cactus plants can not only be eaten raw, but also...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G1/00
Inventor 王茜
Owner 王茜
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