Application of salvianolic acid A freeze-dried powder to preparation of drug for inhibiting neuronal damage or death of brain tissue

A technology of neuron damage and freeze-dried powder injection, which is applied in the direction of carboxylate preparation, freeze-dried delivery, drug combination, etc., can solve the problems of instability of injections and the inability to guarantee the pharmacological effects of salvianolic acid A, so as to improve the transformation rate effect

Active Publication Date: 2013-06-12
JIANGZI QINGFENG PHARMACEUTICALS INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are not many literatures on salvianolic acid A preparations, especially injections, and the instability of injections is common, so the pharmacological effects of salvianolic acid A cannot be guaranteed.

Method used

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  • Application of salvianolic acid A freeze-dried powder to preparation of drug for inhibiting neuronal damage or death of brain tissue
  • Application of salvianolic acid A freeze-dried powder to preparation of drug for inhibiting neuronal damage or death of brain tissue
  • Application of salvianolic acid A freeze-dried powder to preparation of drug for inhibiting neuronal damage or death of brain tissue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0099] Take Danshen medicinal material, crush it into 6-mesh granules, add 7 times the amount of 92°C water each time, warm soak and extract 3 times, and stir at a speed of 25 rpm, and warm soak and extract for 3 hours each time; the extract is concentrated under reduced pressure to relative Density 1.20 (60°C), add ethanol to make the alcohol content at 70%, let it stand, filter, the filtrate is decompressed to recover ethanol and concentrate until it has no alcohol smell; add water to dilute to contain salvianolic acid B20mg per 1ml, use 10% for aqueous solution Adjust the pH to 4.0 with sodium hydroxide, add 0.5% ZnCl 2 As a catalyst, heat and transform at 120°C for 4 hours, adjust the pH value of the transformation liquid to 2.5 with 20% phosphoric acid, centrifuge, concentrate the supernatant under reduced pressure to contain 3 mg of salvianolic acid A per 1 ml, and perform HPD-100 macroporous resin column chromatography For separation, the ratio of the loading amount of ...

Embodiment 2

[0102] Take Salvia miltiorrhiza, crush it into particles with a diameter of about 2mm, add 7 times the amount of 90°C water each time, warm soak and extract 3 times, and stir at a speed of 25 rpm, and warm soak and extract for 3 hours each time; the extract is concentrated under reduced pressure to Relative density 1.16 (60°C), add ethanol to make the alcohol content 70%, let stand, filter, the filtrate recovers ethanol under reduced pressure and concentrates until there is no alcohol smell; add water to dilute to contain salvianolic acid B20mg per 1ml, and use 10 mg of salvianolic acid B in the aqueous solution % NaOH to adjust the pH to 3.5, add 0.5% ZnCl 2 As a catalyst, heat and transform at 120°C for 4 hours, adjust the pH value of the transformation liquid to 2.5 with 20% phosphoric acid, centrifuge, concentrate the supernatant under reduced pressure to contain 3 mg of salvianolic acid A per 1 ml, and perform HPD-100 macroporous resin column chromatography For separation...

Embodiment 3

[0105] Take Salvia miltiorrhiza, cut into decoction pieces, add 8 times the amount each time, soak in water at 85°C for 3 times, stir at a speed of 20 rpm, and extract with warm soaking for 2.5 hours each time; the extract is concentrated under reduced pressure to a relative density of 1.20 (60 ℃), add ethanol to make the alcohol content at 75%, let it stand, filter, and the filtrate reclaims ethanol under reduced pressure and concentrates to no alcohol smell; add water to dilute to contain salvianolic acid B15mg per 1ml, adjust the aqueous solution with 10% potassium hydroxide pH to 5.0, add 0.6% ZnCl 2 As a catalyst, heat conversion at 120°C for 3.5 hours, adjust the pH value of the conversion liquid to 2.5 with 15% hydrochloric acid, centrifuge, concentrate the supernatant under reduced pressure to contain 5 mg of salvianolic acid A per 1 ml, pass through HPD-100 macroporous resin column layer Analysis and separation, the ratio of the loading amount of salvianolic acid A to...

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Abstract

The invention relates to application of a salvianolic acid A freeze-dried powder to preparation of a drug for inhibiting neuronal damage or death of brain tissue. The salvianolic acid A freeze-dried powder contains a salvianolic acid A main ingredient, a filler and an antioxidant in the following weight proportion: 20g-60g of the salvianolic acid A main ingredient, 20g-60g of the filler and the antioxidant accounting for 0.02%-0.1% of the total amount. The salvianolic acid A main ingredient comprises 94%-97% of salvianolic acid A, 0.2%-1.5% of lithospermic acid, 0.2%-1.5% of rosmarinic acid, 0.2%-1.5% of salvianolic acid B and 0.4%-2% of salvianolic acid C.

Description

technical field [0001] The invention relates to the use of salvianolic acid A freeze-dried powder injection for preparing a medicine for inhibiting brain tissue neuron damage or death. technical background [0002] Cerebrovascular disease, also known as stroke, is a neurological disease caused by various causes of blood vessels supplying the brain. The main cause is cerebral arterial lumen stenosis, vasospasm, occlusion or rupture, reduced or complete blockage of blood flow, brain blood circulation and dysfunction caused by cerebral arterial system damage (such as cerebral arteriosclerosis), and brain tissue damage. A series of symptoms caused by damage. Mainly including ischemic and hemorrhagic cerebrovascular diseases. Among them (ICVD, also known as ischemic stroke) accounted for about 80%. [0003] Ischemic cerebrovascular disease refers to the degeneration, necrosis or transient loss of function of local brain tissue including nerve cells, glial cells and connecting ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/343A61K31/216A61K9/19A61P25/00A61P9/10A61P7/02A61P25/28A61P7/10A61P39/06C07C69/732C07C67/32
Inventor 文萍张功俊吕武清刘艳红欧阳婷崔刚蒋春红
Owner JIANGZI QINGFENG PHARMACEUTICALS INC
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