Application of turmeric native

A technology of curcumin and neurotoxin, applied in nervous system diseases, drug combinations, active ingredients of ketones, etc., can solve problems such as unreported effects, achieve great potential value, and reduce the effect of dopaminergic neuron damage

Inactive Publication Date: 2009-02-18
RUIJIN HOSPITAL ATTACHED TO SHANGHAI NO 2 MEDICALUNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that curcumin may have a certain effect on the prevention of PD, but the exact effect has not been reported so far

Method used

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  • Application of turmeric native
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  • Application of turmeric native

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 , grouping and injection of animals

[0030] Take 60 healthy 8-week-old male C57BL mice (Shanghai BK Animal Experiment Co., Ltd.), weighing about 20 g, and divide them into 6 groups randomly, with 10 mice in each group, as follows:

[0031] Normal control group: intraperitoneal injection of normal saline for 12 days;

[0032] MPTP group (model group): after continuous intraperitoneal injection of 30mg / kg MPTP for 5 days, it was continuously injected with normal saline for 7 days;

[0033] Treatment group 1: After continuous intraperitoneal injection of 30 mg / kg MPTP for 5 days, 5 mg / kg curcumin was continuously injected for 7 days;

[0034] Treatment group 2: After continuous intraperitoneal injection of 30 mg / kg MPTP for 5 days, 50 mg / kg curcumin was continuously injected for 7 days;

[0035] Treatment group 3: after continuous intraperitoneal injection of 30 mg / kg MPTP for 5 days, 150 mg / kg curcumin was continuously injected for 7 days;

[0036] Solvent...

Embodiment 2

[0037] Example 2 , immunohistochemical analysis

[0038] 2.1. Immunohistochemical method to detect changes in the expression of TH positive cells in the substantia nigra

[0039] Get the 6 groups of mice of embodiment 1, after intraperitoneal injection of 3% pentobarbital (0.15mL per kilogram of body weight) anesthetized, with 0.9% normal saline 100ml (room temperature) and 4% paraformaldehyde 250ml (4 ℃) via The ascending aorta of the left ventricle was perfused to fix the brain tissue, and the midbrain segment of the rat brain was taken and immersed in fixative solution at 4°C for 24 hours. The fixed midbrain segment of the rat brain was dehydrated with graded ethanol, transparentized with xylene, embedded in paraffin, and then serially coronal paraffin sections (thickness: 6 μm) were performed, and the substantia nigra and striatum of the midbrain were selected for immunohistochemical staining Dry the sheet at 60-65°C for 4 hours, dewax with xylene and graded ethanol to ...

Embodiment 3

[0047] Example 3 , Western blot detection

[0048] 3.1. Effect of curcumin on the positive expression (relative value) of TH protein in substantia nigra

[0049] The substantia nigra tissue isolated from (1)-(6) group mice in Example 1 was added in proportion to the lysate (1mmol / L Tris, 50mmol / L sodium chloride, 0.03μmol / L sodium pyrophosphate, 50mmol / L fluorine NaCl, 1% Triton X-100, 1mmol / L phenylmethylsulfonyl fluoride, 1mmol / L sodium vanadate, 20μg / mL aprotinin), made into 50mg / mL homogenate, centrifuged at 15000r / min 4℃ for 15min , take the supernatant and store it at -80°C, and use the bicinchoninic acid (BCA) method to determine the total protein content in the lysate; add 2×sample buffer to the tissue homogenate to dilute in half, boil for 5min, 6000r / Centrifuge for 3 min, take the supernatant and load the sample, take 30 μg of the sample and separate the protein on the gel by 10% sodium dodecylsulfonate polyacrylamide gel electrophoresis (SDS-PAGE); TH polyclona...

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Abstract

The invention discloses novel application of a medicine - curcumin, namely application of the curcumin in preventing and treating or treating the Parkinson disease. After the curcumin acts on an MPTP-constructed Parkinson disease mouse model, the curcumin can effectively promote expression of tyrosine hydroxylase in substantia nigra, lighten damage of sneurotoxin on the tyrosine hydroxylase in substantia nigra, reduces damage of dopaminergic neurons, promote biosynthesis of damaged dopamine and promote restoration and growth of damaged dopaminergic neurons; the curcumin can also inhibit astrocytes to secrete gelatinous fiber proteins, reduce stimulus of the neurotoxin on the astrocytes and lighten phlegmonosis of a brain tissue; moreover, the curcumin can inhibit expression of induction type nitrous oxide synthase, lighten induction of the neurotoxin on the induction type nitrous oxide synthase and inhibit reinforcement of intracephalic oxidative stress reaction. Therefore, the curcumin can effectively reduce damage of the dopaminergic neurons, thereby having significant potential value in developing medicines for preventing and treating and / or treating the Parkinson disease.

Description

technical field [0001] The invention belongs to the field of Chinese herbal medicine, and in particular relates to the application of curcumin in the preparation, treatment and / or prevention of Parkinson's disease. Background technique [0002] Parkinson's disease (PD) refers to a slowly progressive neurodegenerative disease that mostly occurs in middle-aged and elderly people. It is reported abroad that its prevalence rate reaches 1% in people over the age of 55. There are currently about 1 million PD patients in the United States, while the current number of patients in my country is about 2 million. With the aging of the population, this number will increase significantly in the next decade. This has caused countries all over the world to pay attention to PD and invest in research funding. [0003] The main pathological changes of Parkinson's disease are a large number of dopamine (DA) neurons degeneration and death in the substantia nigra compacta of the midbrain, and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/12A61P25/16
Inventor 陈生弟潘静
Owner RUIJIN HOSPITAL ATTACHED TO SHANGHAI NO 2 MEDICALUNIV
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