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Method for preparing seaweed plant vegetative growth regulating agent by using biological enzymolysis method

A growth regulator, seaweed technology, applied in fertilizer mixtures, fertilization devices, applications, etc., can solve the problems of insufficient nutrition and less active ingredients of natural seaweed, and achieve the improvement of the ability of plants to absorb nutrients, improve the quality of crops, and save labor. Effect

Active Publication Date: 2014-12-10
山东嘉丰海洋生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this patent still uses the waste of seaweed chemical enterprises as the initial raw material, and mixes a large amount of other waste residues. The obtained seaweed fertilizer has less natural seaweed active ingredients and insufficient nutrition.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1, raw material: dry kelp (artificial culture)

[0038] Remove the sand and impurities from the dried kelp, steam the kelp for 20-30 minutes to soften the kelp; then add fresh water to soak the softened kelp for 3 hours. The ratio of adding fresh water is 1:5 of dry kelp to fresh water. After soaking, rinse the rehydrated kelp with fresh water, and then use a pulverizer to crush the rehydrated kelp into kelp pulp with a particle size diameter of less than 3mm.

[0039]Weigh 200kg of kelp pulp into the enzymatic hydrolysis tank, adjust the pH to 6.0-7.0, heat up and keep warm at 50-55°C, add 1600g of cellulase that has been dissolved in advance with an enzyme activity of 1.4 million U / g, stir evenly, and enter the first grade enzymolysis. Stir and enzymatic hydrolysis for 5 hours, stop stirring, and enter the secondary enzymatic hydrolysis. Adjust pH6.0-7.0 during secondary enzymolysis, heat up and keep warm at 50-55°C, add 400 grams of papain with dissolve...

Embodiment 2

[0041] Embodiment 2, raw material: fresh kelp (artificial culture)

[0042] Fresh kelp is rinsed and soaked with fresh water to remove sand and salt, and then 200 kg is weighed and crushed into kelp pulp with a particle size diameter of less than 3 mm. Add the kelp pulp into the enzymatic hydrolysis tank, adjust the pH to 6.0-7.0, raise the temperature and keep it warm at 50-55°C, add 6000g of dissolved cellulase with an enzyme activity of 160,000 U / g, stir well, and enter the first-level enzymatic hydrolysis . Stirring and enzymatic hydrolysis for 6 hours, stop stirring, and enter the secondary enzymatic hydrolysis. During the secondary enzymolysis, adjust the pH to 6.0-7.0, heat up and keep warm at 50-55 ° C, add 600 grams of papain with a dissolved activity of 600,000 U / g, stir evenly, stir and enzymolyze for 4 hours, stop stirring, and enter the third stage. grade enzymatic hydrolysis. Three-stage enzymatic hydrolysis to adjust the pH to 3.5-6.0, heat up and keep warm a...

Embodiment 3

[0043] Embodiment 3, raw material: fresh wakame

[0044] Fresh wakame cultivated artificially is rinsed and soaked with fresh water to remove sand and salt, and then 200 kg is weighed and crushed into wakame pulp with a particle size diameter of less than 3 mm. Add the wakame pulp into the enzymatic hydrolysis tank, adjust the pH to 6.0-7.0, raise the temperature and keep it warm at 50-55°C, add 2000g of cellulase with a dissolved activity of 1.4 million U / g, stir evenly, and enter the first-level enzymatic hydrolysis. Stir and enzymatic hydrolysis for 4 hours, stop stirring, and enter the secondary enzymatic hydrolysis. During secondary enzymolysis, adjust the pH to 6.0-7.0, heat up and keep warm at 50-55°C, add 200 grams of dissolved papain with an activity of 800,000 U / g, stir evenly, stir, enzymolysis for 6 hours, stop stirring, Enter the third stage of enzymatic hydrolysis. Three-stage enzymatic hydrolysis to adjust the pH to 3.5-6.0, heat up and keep warm at 50-55°C, a...

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Abstract

The invention relates to a method for preparing a seaweed plant vegetative growth regulating agent by using a biological enzymolysis method. The method comprises the following steps of: pre-treating fresh seaweeds or dry seaweeds and crushing the seaweeds into seaweed slurry; respectively carrying out first-grade, second-grade and third-grade enzymolysis by utilizing cellulase, papain and pectinase, wherein the enzymolysis temperature in each grade is 50-55 DEG C and the enzymolysis lasts for 4-6 hours; and after the enzymolysis is finished, filtering and concentrating enzymatic hydrolysate until that the density of the enzymatic hydrolysate is 1.2-1.3 grams per cubic centimeter, so as to obtain the seaweed plant vegetative growth regulating agent. The product disclosed by the invention is used as a plant growth regulating agent; and the method disclosed by the invention has no pollution, improves the activity and the stability of cytex and obviously improves the output of applied crops.

Description

technical field [0001] The invention relates to a method for preparing a seaweed plant vegetative growth regulator by a biological enzymatic hydrolysis method, and belongs to the technical field of biotechnology and plant nutrition. Background technique [0002] China is a large agricultural country, and the use of chemical fertilizers ranks first in the world. Especially in the past ten years, China has invested heavily in chemical fertilizers, which has caused soil compaction to worsen and worsened, while crop yields and quality have decreased instead. A large amount of chemical fertilizers has caused waste of resources. The aggravation of pollution and environmental pollution, and the growing gap with international low-carbon and environmentally friendly agriculture, the resulting problem of agricultural product residues has threatened human health. Chinese agricultural products are suffering from international green and organic agriculture. To fundamentally solve the uns...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05G3/00
Inventor 郭德强张西森潘云平李居平
Owner 山东嘉丰海洋生物科技有限公司
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