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Preparation method and application of epigallocatechin gallate

A technology of epigallocatechin and gallate, which is applied in the field of medicine, can solve the problems that the use of gallate needs to be further studied, and achieve the effect of improving efficiency

Active Publication Date: 2013-06-12
WUHAN HUADA PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the preparation method and application of epigallocatechin gallate still need to be further studied

Method used

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  • Preparation method and application of epigallocatechin gallate
  • Preparation method and application of epigallocatechin gallate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1: Preparation of Epigallocatechin Gallate

[0038] Add 50g of green tea medicinal material, add 6 times the amount of 80% ethanol, heat and reflux extract 4 times, each time for 0.5 hours, and combine the extracts. The extract was recovered under reduced pressure at 35 degrees Celsius to remove ethanol, dissolved in water and extracted once with an equal volume of ethyl acetate. After extraction, the ethyl acetate layer was subjected to reduced pressure treatment at 40 degrees Celsius in order to obtain green tea extract. The green tea extract was extracted with pure After the water is dissolved, filter and make a solution of 0.5g medicinal material / g resin, put it on a macroporous resin column, elute with 6 column volumes of water, and then use 4 column volumes of 15% ethanol to elute, collect the ethanol for elution part, dried under reduced pressure to obtain the crude extract of epigallocatechin gallate; add methanol / isopropanol mixed solution (methanol and...

Embodiment 2

[0039] Example 2: Preparation of Epigallocatechin Gallate

[0040] Add 50g of green tea medicinal material, add 15 times the amount of 30% ethanol, heat and reflux for extraction once, each time for 3 hours, and combine the extracts. The extract was recovered under reduced pressure at 70 degrees Celsius to remove ethanol, dissolved in water and extracted 4 times with an equal volume of ethyl acetate. After extraction, the ethyl acetate layer was subjected to reduced pressure treatment at 90 degrees Celsius in order to obtain green tea extract. The green tea extract was extracted with pure After the water is dissolved, filter and make a solution of 3g medicinal materials / g resin, put it on a macroporous resin column, elute with 2 column volumes of water, and then elute with 5% ethanol for 8 column volumes, collect the ethanol eluted part , dried under reduced pressure to obtain the crude extract of epigallocatechin gallate; add methanol / isopropanol mixed solution (the volume of...

Embodiment 3

[0041] Example 3: Preparation of Epigallocatechin Gallate

[0042] Add 50g of green tea medicinal material, add 8 times the amount of 70% ethanol, heat and reflux for extraction 3 times, each time for 1 hour, and combine the extracts. The extract was recovered under reduced pressure at 60 degrees Celsius to remove ethanol, dissolved in water and extracted three times with an equal volume of ethyl acetate. After extraction, the ethyl acetate layer was subjected to reduced pressure treatment at 60 degrees Celsius to obtain green tea extract. After the water is dissolved, filter and make a solution of 2g medicinal material / g resin, put it on a macroporous resin column, elute with 6 column volumes of water, and then elute with 8 column volumes of 10% ethanol, collect the ethanol eluted part , dried under reduced pressure to obtain crude epigallocatechin gallate extract; add methanol / isopropanol mixed solution (the volume of methanol and isopropanol The ratio is 2:1, the weight of...

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Abstract

The invention relates to a preparation method and an application of epigallocatechin gallate. The method comprises the following steps of: adding alcohol to green tea, and extracting to obtain green tea extraction liquid; purifying the green tea extraction liquid to obtain a green tea extract; purifying the green tea extract to obtain a crude extract of epigallocatechin gallate; and recrystallizing the crude extract of epigallocatechin gallate to obtain epigallocatechin gallate. The method provided by the invention can be used for effectively preparing epigallocatechin gallate, and the product can be used for preparing a medicine, a healthcare food or food for preventing and treating anemia.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a preparation method and application of epigallocatechin gallate. Background technique [0002] The main active ingredient of green tea is tea polyphenol (tea polyphenol, TP), also known as tea tannin and catechin, which is the general term for catechins, flavonoids, phenolic acids and anthocyanins in tea, accounting for about The dry weight of tea leaves is 18-36%. A large number of studies have shown that tea polyphenols have strong antioxidant capacity and have been used as natural food antioxidant additives. The research on the chemical composition of tea polyphenols shows that the main components of tea polyphenols are catechin compounds, accounting for about 70% to 80% of the total amount of tea polyphenols, mainly containing catechin (C), epicatechin Epigallocatechin (EC), epicatechin gallate (ECG), epigallocatechin gallate (EGC) and epigallocatechin gallate ...

Claims

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Application Information

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IPC IPC(8): C07D311/62A61K31/353A61P7/06
CPCC07D311/62A61K31/353A61P7/06
Inventor 黄璐杨爽张思佳牟峰汪建
Owner WUHAN HUADA PHARMA
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