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Column type immobilized reactor and process for producing rhamnose gum by fermentation

A technology of rhamnose gum and stirred reactor, which is applied in the direction of bioreactor/fermenter combination, biochemical instrument, bioreactor/fermenter for specific purposes, etc., and can solve the problem of difficult high-viscosity fermentation of rhamnose gum , It is not suitable for living cell fermentation, and it is difficult for cells to maintain oxygen transfer and material exchange, etc., to achieve immobilized fermentation production, continuous multi-batch immobilized fermentation production, and high production efficiency

Inactive Publication Date: 2014-08-27
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional immobilization method mainly uses the gel embedding method, but the cells embedded in the gel embedding method are difficult to maintain oxygen transfer and material exchange, and are not suitable for the fermentation of living cells, and it is difficult to achieve high-viscosity fermentation of rhamnose gum

Method used

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  • Column type immobilized reactor and process for producing rhamnose gum by fermentation
  • Column type immobilized reactor and process for producing rhamnose gum by fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: A 3L stirred reactor uses glucose as a carbon source to ferment and produce rhamnose gum in a single batch of free cells (without an external immobilized column).

[0033] Using glucose as carbon source, rhamnan gum was produced by single-batch fermentation of free cells of Sphingomonas sp.RH-1 (CGMCC NO.6833), which has been patented by our laboratory.

[0034] Seed medium: glucose 10g / L, beef extract 3g / L, peptone 5g / L, MgSO 4 ·7H 2 O0.5g / L, use NaOH solution to adjust pH6.8.

[0035] Fermentation medium: glucose 40g / L, yeast extract 5g / L, (NH 4 ) 2 SO 4 5g / L, K 2 HPO 4 ·3H 2 O2g / L, MgSO 4 ·7H 2 O0.5g / L, use NaOH solution to adjust pH6.8.

[0036] Scrape the strains on the slant of fresh Sphingomonas sp.RH-1, inoculate them in fresh seed medium, and cultivate them under the condition of 30°C and 200r / min shaker for 18h, and the seed solution is 8% (v / v) Inoculate in a 3L stirred reactor 11 pre-installed with 2.1L sterilized fermentation medium fo...

Embodiment 2

[0037] Example 2: Fermentation of rhamnose gum in a single batch of immobilized cells in a 3L stirred reactor using glucose as a carbon source.

[0038] Bacterial strain, seed culture medium and fermentation medium are the same as embodiment 1. using the device of the present invention.

[0039] Scrape the strains on the slant of fresh Sphingomonas sp.RH-1, inoculate them in fresh seed medium, and cultivate them under the condition of 30°C and 200r / min shaker for 18h, and the seed solution is 8% (v / v) Inoculate in a 3L stirred reactor 11 pre-installed with 2.6L sterilized fermentation medium for cultivation, turn on the constant flow pump 2-3 to realize the material exchange between the stirred reactor 11 and the immobilized column 12, turn on The constant temperature water bath 14 realizes that the temperature of the material in the immobilized column 12 is constant at 30°C. The reaction conditions of the stirred reactor are: 30°C, 600r / min, and the ventilation rate is 2.3L / ...

Embodiment 3

[0040] Example 3: Fermentation of rhamnose gum by multi-batch immobilized cells in a 3L stirred reactor using glucose as a carbon source.

[0041] Bacterial strain, seed culture medium and fermentation medium are the same as embodiment 1. using the device of the present invention.

[0042] Scrape the strains on the slant of fresh Sphingomonas sp.RH-1, inoculate them in fresh seed medium, and cultivate them under the conditions of 30°C and 200r / min shaker for 18h, and the seed solution is 10% (v / v) of the seed amount Inoculate in a 3L stirred reactor 11 pre-installed with 2.6L sterilized fermentation medium for cultivation, turn on the constant flow pump 2-3 to realize the material exchange between the stirred reactor 11 and the immobilized column 12, turn on The constant temperature water bath 14 realizes that the temperature of the material in the immobilized column 12 is constant at 32°C. The reaction conditions of the stirred reactor are: 32°C, 1000r / min, and the ventilati...

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Abstract

The invention discloses a column-type fixed reactor for fermentation and production of rhamnose gum. The device is composed of a stirring reactor part and a fixed column part. The two parts are connected through a constant flow pump for material exchange; the stirring-type reaction The reactor part is composed of a stirred reactor, a pH automatic control device and a feeding device, a first ventilation device and a stirring device; the fixed column part is composed of a fixed column, a constant temperature water bath and a second ventilation device. The invention also discloses a process for fermenting and producing rhamnose gum using the above-mentioned column-type immobilized reactor. The process of the invention has the advantages of high rhamnose gum production intensity and high product concentration, is conducive to industrialization, and is an efficient production method.

Description

technical field [0001] The invention belongs to the technical fields of fermentation engineering and process engineering, and relates to a column-type immobilized reactor for fermenting and producing rhamnose gum and a process thereof. Background technique [0002] Rhamnan Gum (Rhamsan Gum) is an exopolysaccharide produced by Sphingomonas, a kind of gellan gum polysaccharide, also known as S-194. Gellan gum polysaccharides have the same main chain structure of four sugar repeating units: D-glucose, D-glucuronic acid, D-glucose and L-rhamnose, but there are some slight differences in side chains or other structures. The structure of rhamnose gum is shown in (1). With its unique physical and rheological properties and safety in use, rhamnose gum can be widely used as suspending agent, stabilizer, thickener, emulsifier, film-forming agent, etc. in food, petroleum, cement, coatings, etc. industry. [0003] [0004] At present, the research on rhamnose gum in the world is s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/36C12M1/34C12M1/04C12M1/02
Inventor 徐虹徐晓滢朱萍李莎陈晓晔
Owner NANJING TECH UNIV