Microporous grid plate separation type multi-tissue culture dish

Abstract

The invention relates to a microporous grid plate separation type multi-tissue culture dish. The microporous grid plate separation type multi-tissue culture dish comprises a tissue culture dish, a microporous screen embedding-type lobulated grid plates and a dish cover and is prepared from high-purity protogenic polystyrene accordant with USP (United States Pharmacopoeia) class VI standards in a once forming manner. According to the microporous grid plate separation type multi-tissue culture dish, different tissue cells can be dispersed and inoculated in different partitioned cells; and by adopting microporous grid plates, the leakage and the mixing of the cells in the different partitioned cells are avoided, the cross contamination is further avoided, and meanwhile, the free osmosis and convective diffusion of fluid nutrient media are permitted, so that metabolic products of the cells in the different partitioned cells in a same culture system can be interchanged to finish a synergistic activation effect, the efficient and independent multiplication, recovery and separation of target amplification cells are facilitated, the pollution is reduced, and the efficiency is improved.

Description

technical field [0001] The invention relates to a microporous grid-isolated multi-tissue culture dish, in particular to a micro-porous grid-isolated multi-tissue culture dish used for the isolation and co-cultivation of various tissue cells. Background technique [0002] Tissue cell culture on tissue culture dishes has been widely used in fields such as virology, drug filtration and tissue engineering. Existing professional suppliers have maximized their design and production to ensure the convenience, safety, efficiency and uniformity of the application, but their application is mainly for the mixed culture of a single tissue or multiple tissues in the same space, for such as monoclonal Complex scientific research or clinical applications such as filtration of antibody (McAb) hybridoma cells, clonal proliferation, or in vitro activation of dendritic cells (DC) and cytokine-induced killer cells (CIK) for tumor combination therapy are still insufficient. The new type of tiss...

AI Technical Summary

Problems solved by technology

Existing professional suppliers have maximized their design and production to ensure the convenience, safety, efficiency and uniformity of the application, but their application is mainly for the mixed culture of a single tissue or multiple tissues in the same space, for such as monoclonal Complex scientific research or clinical application such as filtration of McAb hybridoma cells, clonal proliferation, or in vitro activation of dendritic cells (DC) and cytokine-induced killer cells (CIK) for tumor combination therapy are still insufficient

The new type of tissue culture not only requires the co-cultivation of multiple tissue cells in the early stage to achieve mutual nutritional synergies, but also needs to separate and purify (enrich) the target expanded cells in the later stage. Separation and purification operations are carried out for different characteristics in the cycle. The existing culture method adds a lot of digestion or bottle transfer operations, which is time-consuming and laborious. It prolongs the in vitro time of the activated effector cells, affects their vitality, and also increases the chance of contamination.

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