Microporous grid plate separation type multi-tissue culture dish

A technology of tissue culture and petri dish, applied in the direction of tissue cell/virus culture device, enzymology/microbiology device, bioreactor/fermenter combination, etc., can solve the problem of affecting vitality, increasing the chance of contamination, scientific research or clinical Insufficient application and other problems to achieve the effect of improving efficiency and reducing pollution

Inactive Publication Date: 2013-06-12
TAISHAN MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Existing professional suppliers have maximized their design and production to ensure the convenience, safety, efficiency and uniformity of the application, but their application is mainly for the mixed culture of a single tissue or multiple tissues in the same space, for such as monoclonal Complex scientific research or clinical application such as filtration of McAb hybridoma cells, clonal proliferation, or in vitro activation of dendritic cells (DC) and cytokine-induced killer cells (CIK) for tumor combination therapy are still insufficient
The new type of tissue cul

Method used

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  • Microporous grid plate separation type multi-tissue culture dish
  • Microporous grid plate separation type multi-tissue culture dish

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Embodiment

[0013] Such as figure 1 As shown: there is a raised ridge line with a width and a height of 1mm at the bottom edge of the petri dish at a distance of 1mm from the edge, which is convenient to bite and fix with the groove on the upper surface of the dish cover, and is convenient for stacking and discharging of multiple closed petri dishes; the outer side of the petri dish is 2 The / 3 base is 1mm wider to add a fit for the lid of the dish.

[0014] The petri dish is cylindrical, and the inner bottom of the petri dish is gently connected with the inner wall in a 45° arc to reduce the edge effect when the cells are subpackaged and cultured.

[0015] The center of the petri dish is vertically bonded and fixed with an evenly divided page-type microporous grid plate with a height of 80% and a hole depth of 80%. The joints between the pages, the joints between the grid plate and the bottom surface of the petri dish and the side wall are also treated in a 45° arc type to reduce the ed...

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Abstract

The invention relates to a microporous grid plate separation type multi-tissue culture dish. The microporous grid plate separation type multi-tissue culture dish comprises a tissue culture dish, a microporous screen embedding-type lobulated grid plates and a dish cover and is prepared from high-purity protogenic polystyrene accordant with USP (United States Pharmacopoeia) class VI standards in a once forming manner. According to the microporous grid plate separation type multi-tissue culture dish, different tissue cells can be dispersed and inoculated in different partitioned cells; and by adopting microporous grid plates, the leakage and the mixing of the cells in the different partitioned cells are avoided, the cross contamination is further avoided, and meanwhile, the free osmosis and convective diffusion of fluid nutrient media are permitted, so that metabolic products of the cells in the different partitioned cells in a same culture system can be interchanged to finish a synergistic activation effect, the efficient and independent multiplication, recovery and separation of target amplification cells are facilitated, the pollution is reduced, and the efficiency is improved.

Description

technical field [0001] The invention relates to a microporous grid-isolated multi-tissue culture dish, in particular to a micro-porous grid-isolated multi-tissue culture dish used for the isolation and co-cultivation of various tissue cells. Background technique [0002] Tissue cell culture on tissue culture dishes has been widely used in fields such as virology, drug filtration and tissue engineering. Existing professional suppliers have maximized their design and production to ensure the convenience, safety, efficiency and uniformity of the application, but their application is mainly for the mixed culture of a single tissue or multiple tissues in the same space, for such as monoclonal Complex scientific research or clinical applications such as filtration of antibody (McAb) hybridoma cells, clonal proliferation, or in vitro activation of dendritic cells (DC) and cytokine-induced killer cells (CIK) for tumor combination therapy are still insufficient. The new type of tiss...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12M1/22
Inventor 王玉姜世金鹿秀霞于爱莲施鲁笛
Owner TAISHAN MEDICAL UNIV
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