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Method for preparing citric acid

A technology of citric acid and high temperature-resistant amylase, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low citric acid fermentation efficiency, achieve inhibition of microbial growth, high starch utilization rate, The effect of improving fermentation efficiency

Active Publication Date: 2013-06-12
COFCO BIOTECHNOLOGY CO LTD
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  • Abstract
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Problems solved by technology

Thereby making the fermentation efficiency when subsequent fermentation makes citric acid is lower

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  • Method for preparing citric acid

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preparation example Construction

[0020] The method for preparing citric acid provided by the invention may further comprise the steps:

[0021] (1) Prepare an aqueous solution containing high-temperature-resistant amylase, mix the aqueous solution containing high-temperature-resistant amylase with starchy raw material powder to obtain a mixture, and the mixing conditions include a temperature of 65-90°C, preferably 70-85°C;

[0022] (2) Spray the mixture obtained in step (1) once to 90-100°C and keep it; then spray the product obtained once to 120-140°C and keep it;

[0023] (3) Under enzymatic hydrolysis conditions, mixing the product obtained in step (2) with amylase for enzymatic hydrolysis to obtain a liquefied liquid containing oligosaccharides;

[0024] (4) Using the liquefied liquid to prepare a fermentation medium, and inoculating Aspergillus niger into the fermentation medium under the condition that citric acid can be produced for fermentation.

[0025] In step (1), as long as the mixing conditions...

Embodiment 1

[0059] (1) Dissolve the high-temperature-resistant amylase with an amount of 35 enzyme activity units / g corn flour in water, and mix the obtained high-temperature-resistant amylase aqueous solution with 100 parts by weight of corn flour (with an average particle size of 400 μm) at 70°C ) are mixed to obtain a mixture;

[0060] (2) Inject the mixture and saturated steam at 150°C once in the Megalight injector (the weight ratio of steam to mixture is 0.05:1), and the time of one injection is 5s, so that the temperature of the product after contacting with steam is 90°C, and the laminar flow was maintained for 120 minutes; the product obtained from the first injection and the supersaturated steam at 220°C were injected twice in the Zhaoguang injector (the weight ratio of steam to the product obtained from the first injection was 0.05:1), The time of the secondary injection is 5s, so that the temperature of the product after contacting with steam is 120°C, and it is kept at this t...

Embodiment 2

[0064] (1) Dissolve the high-temperature-resistant amylase with an amount of 25 enzyme activity units / g corn flour in water, and mix the obtained high-temperature-resistant amylase aqueous solution with 100 parts by weight of corn flour (with an average particle size of 400 μm) at 85°C ) are mixed to obtain a mixture;

[0065] (2) The mixture and saturated steam at 160°C are sprayed once in the Megalight injector (the weight ratio of steam to mixture is 0.05:1), and the time of one injection is 5s, so that the temperature of the product after contacting with steam is 100°C, and maintain the laminar flow for 60 minutes; then inject the product obtained from the first injection and the supersaturated steam at 220°C in the Megalight injector for a second injection (the weight ratio of steam to the product obtained from the first injection is 0.06:1), The time of the secondary injection is 5s, so that the temperature of the product after contacting with steam is 140°C, and keep at...

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Abstract

The invention discloses a method for preparing citric acid, which comprises the following steps of: (1) preparing aqueous solution containing high-temperature resistant amylase, and mixing the aqueous solution containing the high-temperature resistant amylase with starchy material powder to obtain a mixture, wherein mixing is carried out at 65-90 DEG C, preferably 70-85 DEG C; (2) carrying out one-time injection of the mixture obtained in the step (1) to 90-100 DEG C and keeping, and carrying out two-time injection of the product obtained through one-time injection to 120-140 DEG C and keeping; (3) mixing the product obtained in the step (2) with amylase under the condition of enzymatic hydrolysis, and carrying out enzymatic hydrolysis to obtain liquefied solution containing oligosaccharide; and (4) preparing a fermentation culture medium by utilizing the liquefied solution, and inoculating aspergillus niger into the fermentation culture medium to ferment under the condition of generating citric acid. The method disclosed by the invention is capable of effectively inhibiting growth of micro-organisms in the size mixing process and effectively increasing the fermentation efficiency of citric acid; and the liquefaction effect is steady and thorough.

Description

technical field [0001] The invention relates to a method for preparing citric acid, in particular to a method for fermenting citric acid using the liquefied liquid prepared in the process of the invention. Background technique [0002] At present, the method for producing citric acid by fermentation in domestic enterprises is generally as follows: prepare liquefied supernatant, then use the obtained liquefied supernatant to prepare fermentation medium, inoculate Aspergillus niger into the fermentation medium for fermentation, and obtain citric acid fermentation broth. The method for preparing the liquefied serum includes adding corn flour to water at a temperature of 50-60° C. to prepare a slurry to obtain a slurry, then adding calcium hydroxide to adjust the pH of the slurry to 6.0-6.5, and adding amylase, spraying once to 80-95°C, press filter after maintaining the iodine test, and the obtained filtrate is liquefied clear liquid. [0003] The above-mentioned method for pr...

Claims

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Application Information

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IPC IPC(8): C12P7/48C12P19/14C12R1/685
Inventor 李北罗虎熊结青朱继成章辉平
Owner COFCO BIOTECHNOLOGY CO LTD
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